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2.4.1.19: cyclomaltodextrin glucanotransferase

This is an abbreviated version!
For detailed information about cyclomaltodextrin glucanotransferase, go to the full flat file.

Word Map on EC 2.4.1.19

Reaction

alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranose
=
alpha-cyclodextrin
 +
alpha-D-glucose

Synonyms

1,4-alpha-D-glucopyranosyl transferase, Akrilex C cyclodextrin glycosyltransferase, alpha-1,4-glucan 4-glycosyltransferase, cyclizing, alpha-cgt, alpha-CGTase, alpha-cyclodextrin glucanotransferase, alpha-cyclodextrin glucosyltransferase, alpha-cyclodextrin glycosyltransferase, Bacillus macerans amylase, beta-CGTase, beta-cyclodextrin glucanotransferase, beta-cyclodextrin glucosyltransferase, beta-cyclodextrin glycosyltransferase, beta-cyclodextrinase, BMA, C-CGTase, CD glucanotransferase, CGT, CGT13, CGTase, cgtS, CGTse ET1, CGT_TK, cyclodextrin beta-glucanotransferase, cyclodextrin glucanotransferase, cyclodextrin glucosyltransferase, cyclodextrin glycosyl transferase, cyclodextrin glycosyltransferase, cyclodextrin-glycosyltransferase, cyclodextrinase, cyclomaltodextrin glucanyltransferase, cyclomaltodextrin glucotransferase, cyclomaltodextrin glycanotransferase, cyclomaltodextrin glycosyltransferase, gamma-CGTase, gamma-cyclodextrin glycosyltransferase, konchizaimu, M-CGTase, More, neutral-cyclodextrin glycosyltransferase, PFCGT, Toruzyme

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.19 cyclomaltodextrin glucanotransferase

Activating Compound

Activating Compound on EC 2.4.1.19 - cyclomaltodextrin glucanotransferase

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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(CH3COO)2Pb
Bacillus autolyticus
-
relative activity 103%
2-mercaptoethanol
Bacillus autolyticus
-
relative activity 101%
Benzene
-
relative activity 106%
bromobenzene
Ca2+
-
4 mM, 22% activation
chitosan
-
the beta-CGTase and gamma-CGTase activities increase as the chitosan concentrations increases to 2% (w/v)
cyclohexadec-8-ene-1-one
Cyclohexane
cyclotridecanone
decane
-
increases the yield of cyclodextrin
Decanol
Dextrin
-
favores CGTase synthesis
dithiothreitol
Bacillus autolyticus
-
relative activity 101%
DTT
-
slight activation
ethanol
ethylene glycol
-
with increasing concentrations of ethylene glycol (up to 20% v/v), the beta-CGTase activity increases by 9.8%, 65.4%, and 35.1%, respectively, and the gamma-CGTase activity increases by 7.8%, 66.7%, and 39.0%, respectively, compared with that of control
fructose
-
favores CGTase synthesis
galactose
-
favores CGTase synthesis
gelatin
-
with increasing gelatin concentrations, both the beta-CGTase and gamma-CGTase activities show bell-shaped curves, with the highest increases of activities of 48.9% and 38.6%, respectively, at 1% (w/v) gelatin
-
glucose
-
favores CGTase synthesis
glycerol
-
with increasing concentrations of glycerol (up to 20% v/v), the beta-CGTase activity increases by 9.8%, 65.4%, and 35.1%, respectively, and the gamma-CGTase activity increases by 7.8%, 66.7%, and 39.0%, respectively, compared with that of control
Isopropanol
lactose
-
favores CGTase synthesis
light
-
illumination of the enzyme with white, linearly polarized light and at constant 25°C increases the enzyme activity producing a mixture of alpha-, beta-, and gamma-cyclodextrins. At a high enzyme concentration of 0.64 U/cm3, regardless the illumination time, formation of beta-cyclodextrin predominates. The highest yield of beta-cyclodextrin is afforded after 1 h illumination, but 2 h illumination leads to a significant increase in the yield of gamma-cyclodextrin
-
maltodextrin
-
favores CGTase synthesis
maltose
-
favores CGTase synthesis
mannitol
-
favores CGTase synthesis
mycose
-
the beta-CGTase and gamma-CGTase activities increase as the mycose concentrations increases to 2% (w/v)
n-butanol
NaCl
enzyme shows its maximum hydrolysis activity in a buffer with 1.5 M NaCl and retains up to 65% of its activity at 0.5 M NaCl
Octanol
p-chloromercuribenzoic acid
-
80 mM, relative activity 118%
PEG 3000
-
10% w/v, increases cyclodextrin production
PEG400
-
the beta-CGTase and gamma-CGTase activities increase by 50.4% and 55.3% compared with that of control at the condition of 10% (w/v) PEG400
pentadecane
phenylmethylsulfonyl fluoride
-
80 mM, relative activity 114%
Sodium acetate
-
the highest increase in beta-CGTase activity is 46.9% at 1 mM sodium acetate which is 5.8fold higher than that of the control. The highest increase in gamma-CGTase activity is 39.1% at 2 mM sodium acetate which is 5.0fold higher than that of the control
sorbitol
starch
-
favores CGTase synthesis
sucrose
-
favores CGTase synthesis
tert-butanol
-
strain 251, increases the yield of cyclodextrin
Toluene
Trichloroethylene
Triton X-100
Bacillus autolyticus
-
enhances specificity and yield of beta-cyclodextrin production
undecane
xylose
-
favores CGTase synthesis
additional information
-
synergistic promoting effects of glycine and Ca2+ on the extracellular secretion of the recombinant protein in Escherichia coli, optimal at 150 mM and 20 mM, respectively, overview
-