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2.4.1.207: xyloglucan:xyloglucosyl transferase

This is an abbreviated version!
For detailed information about xyloglucan:xyloglucosyl transferase, go to the full flat file.

Word Map on EC 2.4.1.207

Reaction

2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc =

Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc
+
Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc

Synonyms

Ac(F153)XTH1, Ac(MD2)XTH1, AtXTH12, AtXTH13, AtXTH17, AtXTH18, AtXTH19, AtXTH20, AtXTH21, AtXTH24, BR1, BXET, DcXET1, DcXET2, DcXTH1, DcXTH2, DcXTH3, DcXTH4, DKXTH1, DKXTH2, DkXTH6, DkXTH7, DkXTH8, DlXET1, DlXET2, DlXET3, endo-transglycosylase, endo-xyloglucan transferase, endo-xyloglucanase, endoxyloglucan transferase, EXGT, EXT, FcXTH2, FvXTH6, FvXTH9, GenBank L46792-derived protein GI 950299, GenBank X93173-derived protein GI 1890573, GenBank X93174-derived protein GI 1890575, Genbank X93175-derived protein GI 1890577, GenBank Z97335-derived protein GI, GhXTH1, GhXTH2, GhXTH3, group 3 XTH, GXET, HTG, HvXET3, HvXET4, HvXET6, LeXET2, LeXTH1, MdXTH1, MdXTH10, MdXTH11, MdXTH14, MdXTH3, MdXTH5, MdXTH6, MdXTH7, MdXTH8, MdXTH9, mixed-linkage beta-glucan:xyloglucan endotransglucosylase, MLG:xyloglucan endotransglucosylase, More, MXE, NXET, NXG1, PrXTH1, PtnXTH1, PtoXTH27, PtoXTH34, PtrXTH1, PttXET, PttXET16-34, PttXET16A, SIXTH, SIXTH12, SkXTH1, SlXET4, SlXTH1, SlXTH10, SlXTH11, SlXTH12, SlXTH2, SlXTH3, SlXTH4, SlXTH5, SlXTH6, SlXTH7, SlXTH8, SlXTH9, tXET-B1, tXET-B2, XEH, XET, XET(6.3), XET/H, XET/XTH, XET02, XET16-34, XET16A, XET3, XET4, XET5, XET6, XET6.3, XTH, XTH/XET, XTH1, XTH10, XTH11, XTH12, XTH13, XTH14, XTH15, XTH18, XTH19, XTH2, XTH20, XTH24, XTH26, XTH28, XTH3, XTH30, XTH31, XTH33, XTH4, XTH5, XTH6, XTH7, XTH8, XTH9, xyloglucan endo-transglucosylase, xyloglucan endo-transglycosylase, xyloglucan endo-transglycosylase/hydrolase, xyloglucan endo-transglycosylase/xyloglucan endo-transglycosylase/hydrolase, xyloglucan endo-transglycosylases/hydrolase, xyloglucan endohydrolase, xyloglucan endotransglucosylase, xyloglucan endotransglucosylase 16A, xyloglucan endotransglucosylase-hydrolase30, xyloglucan endotransglucosylase/endohydrolase, xyloglucan endotransglucosylase/hydrolase, xyloglucan endotransglucosylases/hydrolase, xyloglucan endotransglycosylase, xyloglucan endotransglycosylase (Actinia deliciosa strain Hayward pericarp clone AdXET-5 gene XET precursor), xyloglucan endotransglycosylase (Arabidopsis thaliana gene TCH4 precursor reduced), xyloglucan endotransglycosylase (barley clone PM5 gene HVPM5), xyloglucan endotransglycosylase (barley clone XEA gene HVXEA), xyloglucan endotransglycosylase (barley clone XEB gene HVXEB), xyloglucan endotransglycosylase 16A, xyloglucan endotransglycosylase-related protein XTR-7 (Arabidopsis thaliana), xyloglucan endotransglycosylase/hydrolase, xyloglucan endotransglycosylase/hydrolase1, xyloglucan endotransglycosylation activity, xyloglucan hydrolase, xyloglucan recombinase, xyloglucan X93174-derived protein GI 1890575, xyloglucan xyloglucosyl transferase, xyloglucan xyloglucosyl transferases, xyloglucan-specific endo-(1->4)-beta-D-glucanase, xyloglucan:xyloglucanotransferase, xyloglucanase 1, xyloglucanotransferase, xyloglucan (xyloglucan donor), xyloglucanotransferase, xyloglucan (xyloglucan donor) (Actinia chinensis clone AdXET-5 gene XET precursor), xyloglucanotransferase, xyloglucan (xyloglucan donor) (Arabidopsis thaliana gene TCH4 precursor reduced), xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone PM5 gene HVPM5), xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone XEA gene HVXEA), xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone XEB gene HVXEB), ZmXTH1

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.207 xyloglucan:xyloglucosyl transferase

Purification

Purification on EC 2.4.1.207 - xyloglucan:xyloglucosyl transferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
3000fold, AdXET6
-
87fold, from apoplastic space of epicotyls
-
ammonium sulfate precipitation, Hi-Trap SP Sepharose column chromatography, Superdex 75 gel filtration
-
ammonium sulfate precipitation, Sepharose Q column chromatography, phenyl Sepharose column chromatography and Bio-Gel P60 gel filtration
ammonium sulfate precipitation, Sepharose Q column chromatography, phenyl Sepharose column chromatography, chromatofocusing on PBE-94, and Bio-Gel P-60 gel filtration
-
direct elution from the acrylamide gel
-
general, mechanism-based method for purification, about 200fold, step-wise addition of (NH4)2SO4 reveals distinct isoforms
His-tagged recombinant tXET-B2, expressed in Escherichia coli
-
His-Trap chelating column chromatography
immobilized metal affinity chromatography
isozymes sXET and eXET from germinated seeds, i.e. cotyledons, and from epicotyls, respectively, to near homogeneity by ammonium sulfate fractionation, anion exchange chromatography, adsorption and desorption of the enzyme-substrate complex on cellulose, and hydrophobic-interaction chromatography
-
native enzyme by ultrafiltration, dialysis, and gel filtration, recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and dialysis
native enzyme from florets to homogeneity by adsorption chromatography, 55-140fold purification dependent on different assay methods for quantification
Ni-substituted chelating Sepharose FF column chromatography
-
partial, recombinant TCH4, Meri-5, EXGT and XTR9, expressed in Sf9 insect cells
PD-10 column chromatography and UNO Q column chromatography
-
polyanion SI column chromatography, Spheron phosphate column chromatography, and Superdex 75 gel filtration
-
protein extracts from bean cells are prepared
-
proteins are extracted from tomato plants
recombinant AtXTH12 partially from Pichia pastoris by ammonium sulfate fractionation
recombinant AtXTH13 partially from Pichia pastoris by ammonium sulfate fractionation, AtXTH13 is purified to apparent homogeneity by xylogluco-oligosaccharide affinity chromatography
recombinant AtXTH17 partially from Pichia pastoris by ammonium sulfate fractionation
recombinant AtXTH18 partially from Pichia pastoris by ammonium sulfate fractionation
recombinant AtXTH19 partially from Pichia pastoris by ammonium sulfate fractionation
recombinant enzyme from Pichia pastoris by strong cation exchange chromatography and gel filtration in a sequential combination
recombinant enzyme XTH2 12.43fold from Pichia pastoris by anion exchange chromatography, cation exchange chromatography, desalting gel filtration, and affinity chromatography
recombinant extracellular His-/FLAG-tagged wild-type and mutant enzymes expressed from Pichia pastoris strain GS115 by nickel affinity chromatography and desalting gel filtration
recombinant His-tagged enzyme from Saccharomyces cerevisiae INVSc1 cells by nickel affinity chromatography
-
recombinant His-tagged isozyme DkXTH3 from Escherichia coli strain BL21 by affinity chromatography, urea denaturation, refolding dialysis and ultrafiltration
recombinant His-tagged isozyme DkXTH4 from Escherichia coli strain BL21 by affinity chromatography, urea denaturation, refolding dialysis and ultrafiltration
recombinant His-tagged isozyme DkXTH5 from Escherichia coli strain BL21 by affinity chromatography, urea denaturation, refolding dialysis and ultrafiltration
recombinant His-tagged isozyme without the N-terminal leader sequence from Escherichia coli strain BL21 by affinity chromatography, urea denaturation, refolding dialysis and ultrafiltration
recombinant His-tagged mature coding region of isozyme DkXTH1 from Escherichia coli strain BL21 by affinity chromatography, urea denaturation, refolding dialysis and ultrafiltration
recombinant His-tagged XTH1 from Pichia pastoris by anion exchange chromatography and nickel affinity chromatography
recombinant His6-tagged PttXET16A, expressed in Escherichia coli BL21(DE3)
recombinant I, expressed in Pichia pastoris
-
recombinant NtXET-1, expressed in Escherichia coli
recombinant TCH4 protein expressed in Escherichia coli
-
recombinant wild-type and mutant enzymes from Pichia pastoris by ultrafiltration, one or two steps of cation exchange chromatography, dialysis, and adsorption chromatography
Sepharose Q column chromatography, Phenyl-Sepharose chromatography, PBE-94 column chromatography and Bio-Gel P-60 gel filtration
-
SP-Sepharose fast flow column chromatography and S-source 15S gel filtration
-
Supelclean ENVI-8 SPE column gel filtration
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TCH4 and EXGT
-