2.4.2.3: uridine phosphorylase
This is an abbreviated version!
For detailed information about uridine phosphorylase, go to the full flat file.
Word Map on EC 2.4.2.3
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2.4.2.3
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pyrimidine
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nucleoside
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thymidine
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uracil
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5-fluorouracil
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phosphorolysis
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salvage
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orotate
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phosphoribosyltransferase
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phosphorylases
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thymidylate
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fluoropyrimidine
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udp
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5'-deoxy-5-fluorouridine
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5-fluoro-2'-deoxyuridine
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dihydropyrimidine
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acyclonucleoside
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5-fluorouridine
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ribose-1-phosphate
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capecitabine
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5-methyluridine
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dthdpase
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fdurd
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orotidine
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dihydrouracil
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uridine-cytidine
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mete
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diagnostics
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medicine
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synthesis
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drug development
- 2.4.2.3
- pyrimidine
- nucleoside
- thymidine
- uracil
- 5-fluorouracil
-
phosphorolysis
-
salvage
- orotate
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phosphoribosyltransferase
- phosphorylases
- thymidylate
-
fluoropyrimidine
- udp
- 5'-deoxy-5-fluorouridine
- 5-fluoro-2'-deoxyuridine
- dihydropyrimidine
- acyclonucleoside
- 5-fluorouridine
- ribose-1-phosphate
- capecitabine
- 5-methyluridine
- dthdpase
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fdurd
- orotidine
- dihydrouracil
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uridine-cytidine
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mete
- diagnostics
- medicine
- synthesis
- drug development
Reaction
Synonyms
apUP, EC 2.4.2.23, L-UrdPase, More, PcUP1, PcUP2, phosphorylase, uridine, pynpase, pyrimidine nucleoside phosphorylase, pyrimidine phosphorylase, pyrimidine/purine nucleoside phosphorylase, StUPh, udp, UDRPase
ECTree
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KM Value
KM Value on EC 2.4.2.3 - uridine phosphorylase
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0.121
in 50 mM potassium phosphate buffer, pH 7.5, 25°C
0.036
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cosubstrate: alpha-D-ribose-1-phosphate, enzyme from liver
0.046
5-fluorouracil
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cosubstrate: alpha-D-ribose-1-phosphate, enzyme from colon tumor
0.06
5-fluorouracil
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cosubstrate: alpha-D-ribose-1-phosphate, enzyme from colon tumor
0.065
in 50 mM potassium phosphate buffer, pH 7.5, 25°C
0.02
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cosubstrate: 5-fluorouracil, enzyme from liver
0.038
alpha-D-ribose-1-phosphate
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cosubstrate: 5-fluorouracil, enzyme from colon tumor
0.04
alpha-D-ribose-1-phosphate
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cosubstrate: 5-fluorouracil, enzyme from colon tumor
0.087
alpha-D-ribose-1-phosphate
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cosubstrate: uracil, enzyme from colon tumor
0.0489
pH and temperature not specified in the publication
0.215
thymidine
in 50 mM potassium phosphate buffer, pH 7.5, 25°C
0.367
Uracil
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cosubstrate: alpha-D-ribose-1-phosphate, enzyme from colon tumor
6.96
uridine
pH and temperature not specified in the publication
7.55
uridine
pH and temperature not specified in the publication
additional information
additional information
Michaelis-Menten kinetics and Lineweaver-Burk plots
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additional information
additional information
kinetic analysis of UP1 is not consistent with a strong effect of substrate cooperativity
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additional information
additional information
kinetic analysis of UP1 is not consistent with a strong effect of substrate cooperativity
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additional information
additional information
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kinetic analysis of UP1 is not consistent with a strong effect of substrate cooperativity
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additional information
additional information
kinetic analysis of UP2 is not consistent with a strong effect of substrate cooperativity
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additional information
additional information
kinetic analysis of UP2 is not consistent with a strong effect of substrate cooperativity
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additional information
additional information
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kinetic analysis of UP2 is not consistent with a strong effect of substrate cooperativity
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