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2.5.1.56: N-acetylneuraminate synthase

This is an abbreviated version!
For detailed information about N-acetylneuraminate synthase, go to the full flat file.

Word Map on EC 2.5.1.56

Reaction

phosphoenolpyruvate
+
N-acetyl-D-mannosamine
+
H2O
=
phosphate
+
N-acetylneuraminate

Synonyms

(NANA) condensing enzyme, EC 4.1.3.19, N-acetylneuraminate lyase synthase, N-acetylneuraminate pyruvate-lyase (pyruvate-phosphorylating), N-acetylneuraminic acid condensing enzyme, N-acetylneuraminic acid synthase, NANA condensing enzyme, NANA synthase, NANAS, NANS, NeuAc synthase, NeuB, NeuB1, NeuNAc synthase, NmeNANAS, sialic acid synthase, synthase, N-acetylneuraminate

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.56 N-acetylneuraminate synthase

Engineering

Engineering on EC 2.5.1.56 - N-acetylneuraminate synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A506G/G420A
-
increase in specific activity
C914T
-
decrease in specific activity
C914T/G1018A
-
no significant change in specific activity
G1018A
-
no significant change in specific activity
G283A
-
no significant change in specific activity
T74C
-
increase in specific activity
T74C/A506G
-
increase in specific activity
A506G/G420A
-
increase in specific activity
-
C914T
-
decrease in specific activity
-
C914T/G1018A
-
no significant change in specific activity
-
G283A
-
no significant change in specific activity
-
T74C
-
increase in specific activity
-
G133V
naturally occuring mutation of the enzyme causing impaired enzyme activity and the enzyme-deficiency phenotype
K131Q
naturally occuring mutation of the enzyme causing impaired enzyme activity and the enzyme-deficiency phenotype
P189L
naturally occuring mutation of the enzyme causing impaired enzyme activity and the enzyme-deficiency phenotype
Y188H
naturally occuring mutation of the enzyme causing impaired enzyme activity and the enzyme-deficiency phenotype
C182A
-
no activity
C182S
-
no activity
E134A
site-directed mutagenesis
E282A
site-directed mutagenesis
E282A/T285A
site-directed mutagenesis
T285A
site-directed mutagenesis
T285F
site-directed mutagenesis
E134A
-
site-directed mutagenesis
-
E282A
-
site-directed mutagenesis
-
E282A/T285A
-
site-directed mutagenesis
-
T285A
-
site-directed mutagenesis
-
additional information