Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.7.2.1: acetate kinase

This is an abbreviated version!
For detailed information about acetate kinase, go to the full flat file.

Word Map on EC 2.7.2.1

Reaction

ATP
+
acetate
=
ADP
 +
acetyl phosphate

Synonyms

acetate kinase (phosphorylating), acetic kinase, acetokinase, ACK, ackA, AckA1, AckA2, ACKase, AK, ATP-ecoAK, ATP-specific AK, EAK, EutP, EutQ, MM_0495, Sak, short chain fatty acid kinase, StAckA, urkinase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.2 Phosphotransferases with a carboxy group as acceptor
                2.7.2.1 acetate kinase

Inhibitors

Inhibitors on EC 2.7.2.1 - acetate kinase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,4,6-Trinitrobenzene sulfonic acid
-
-
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
6-oxopiperidine-3-carboxylic acid
-
acetate
inhibition by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate. When MgCl2, ADP, and acetate are omitted from the preincubation mixture, there is no detectable loss of activity; inhibition of acetate kinase by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate (all of the components are necessary for maximum inhibition)
acetyl phosphate
adenosine 5'-(gamma-thio)triphosphate
-
AlCl3
inhibition by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate. When MgCl2, ADP, and acetate are omitted from the preincubation mixture, there is no detectable loss of activity; inhibition of acetate kinase by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate (all of the components are necessary for maximum inhibition). The transition state analog, MgADP-aluminum fluoride-acetate, forms an abortive complex in the active site. Protection from inhibition by a non-hydrolyzable ATP analog or acetylphosphate. Preincubation of acetate kinase with MgCl2, AlCl3, NaF, acetate, and either IDP, UDP, or CDP in place of ADP results in almost complete inhibition of activity
ATP-gamma-S
non-hydrolyzable inhibitor
Bromoacetate
-
-
Ca2+
less than 1% residual activity at 1.3 mM
CDP
preincubation of acetate kinase with MgCl2, AlCl3, NaF, acetate, and either IDP, UDP, or CDP in place of ADP results in almost complete inhibition of activity
chlorogenic acid
D-fructose-1,6-bisphosphate
inhibits the activities of isozymes AckA1 and AckA2; inhibits the activities of isozymes AckA1 and AckA2
diethyldicarbonate
diphosphate
about 70% inhibition in the acetate-forming direction and about 90% inhibition in the acetyl phosphate-forming direction
EDTA
complete inhibition at 1.3 mM
eugenyl acetate
66.52% inhibition at 70 mM
glyceraldehyde-3-phosphate
inhibits the activities of isozyme AckA1, but very poorly of isozyme AckA2; inhibits the activities of isozyme AckA1, but very poorly of isozyme AckA2
HgCl2
hydroxylamine
inhibits acetate kinase reaction in a nonlinear and noncompetitive fashion, substantial inhibition at concentrations of 704 mM and minimal inhibition at concentrations of 250 microM hydroxylamine
IDP
preincubation of acetate kinase with MgCl2, AlCl3, NaF, acetate, and either IDP, UDP, or CDP in place of ADP results in almost complete inhibition of activity
iodoacetamide
-
-
iodoacetate
-
-
Iodosobenzoate
-
-
KCl
activity linearly decreases from 100% (at 0 mM added KCl) to 71% at 500 mM added KCl
MgCl2
inhibition by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate. When MgCl2, ADP, and acetate are omitted from the preincubation mixture, there is no detectable loss of activity; inhibition of acetate kinase by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate (all of the components are necessary for maximum inhibition). The transition state analog, MgADP-aluminum fluoride-acetate, forms an abortive complex in the active site. Preincubation of acetate kinase with MgCl2, AlCl3, NaF, acetate, and either IDP, UDP, or CDP in place of ADP results in almost complete inhibition of activity
N-ethylmaleimide
NaF
inhibition by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate. When MgCl2, ADP, and acetate are omitted from the preincubation mixture, there is no detectable loss of activity; inhibition of acetate kinase by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate (all of the components are necessary for maximum inhibition). The transition state analog, MgADP-aluminum fluoride-acetate, forms an abortive complex in the active site. Preincubation of acetate kinase with MgCl2, AlCl3, NaF, acetate, and either IDP, UDP, or CDP in place of ADP results in almost complete inhibition of activity
p-chloromercuribenzoate
p-hydroxymercuribenzoate
-
-
p-mercuribenzoate
phosphate
-
-
phospho-enol-pyruvate
PEP, a downstream intermediate of glycolysis, completely inhibits the activity of both enzymes at concentrations above 30 mM; PEP, a downstream intermediate of glycolysis, completely inhibits the activity of both enzymes at concentrations above 30 mM
pinoresinol
82.6% inhibition at 70 mM
potassium hydroxylamine
-
-
propionate
preincubation with MgCl2, ADP, AlCl3, NaF, and propionate results in almost complete inhibition of activity
propionic acid
sodium hydroxylamine
-
-
trifluoroethanol
leads to reduced growth and acetate content, binding mode by molecular docking
trifluoroethyl butyrate
leads to reduced growth and acetate content, binding mode by molecular docking
UDP
preincubation of acetate kinase with MgCl2, AlCl3, NaF, acetate, and either IDP, UDP, or CDP in place of ADP results in almost complete inhibition of activity
Zn2+
less than 5% residual activity at 1.3 mM
additional information
-