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145300
wild-type, hexamer, sedimentation velocity at pH 9.0
146400
mutant D159N, hexamer, sedimentation velocity at pH 9.0
147400
mutant D159N, hexamer, sedimentation velocity at pH 7.4
156000
sedimentation equilibrium
157000
wild-type, hexamer, sedimentation velocity at pH 6.0
173200
sedimentation equilibrium ultracentrifugation
189000
major form, representing two-thirds of the protein population, gel filtration
25000
6 * 25000, SDS-PAGE
26000
x * 26000, SDS-PAGE
26300
mass spectroscopy, N-terminal sequencing
27500
x * 27500, His6-tagged enzyme, SDS-PAGE
28120
His-tagged protein, monomer, mass spectroscopy
28250
His-tagged protein, monomer, calculated from amino acid sequence
297400
wild-type, oligomer, sedimentation velocity at pH 9.0
32100
mutant D159N, monomer, sedimentation velocity at pH 9.0
32800
wild-type, monomer, sedimentation velocity at pH 9.0
35300
mutant D159N, monomer, sedimentation velocity at pH 7.4
482900
wild-type, oligomer, sedimentation velocity at pH 6.0
59400
wild-type, dimer, sedimentation velocity at pH 6.0
87500
minor form, gel filtration
140000
gel filtration
150000
gel filtration
25800
monomer, SDS-PAGE
25800
monomer, calculated from amino acid sequence
26080
monomer, calculated from amino acid sequence
26080
monomer, mass spectroscopy