2.7.7.42: [glutamine synthetase] adenylyltransferase

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For detailed information about [glutamine synthetase] adenylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.42

2.7.7.42

pii

deadenylylation

nucleotidyltransferase

nmn

uridylyltransferase

spectinomycin

glnk

sulfurylase

2.7.7.1

aminoglycoside-modifying

pii-ump

Reaction

[glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine

Synonyms

adenosine triphosphate:glutamine synthetase adenylyltransferase, adenylyltransferase, adenylyltransferase, glutamine synthetase, ATASE, ATP:glutamine synthetase adenylyltransferase, ATP:[L-glutamate:ammonia ligase (ADP-forming)] adenylyl transferase, GlnE, glutamine synthetase adenylyl transferase, glutamine synthetase adenylyltransferase, glutamine synthetase ATase, glutamine-synthetase adenylyltransferase, GS ATase, [glutamate-ammonia-ligase] adenylyltransferase

ECTree

2 Transferases

2.7 Transferring phosphorus-containing groups

2.7.7 Nucleotidyltransferases

2.7.7.42 [glutamine synthetase] adenylyltransferase

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Results	in table
42	Activating Compound
16628	AA Sequence
1	CAS Registry Number
8	Cloned(Commentary)
17	Cofactor
4	Crystallization (Commentary)
2	Expression
7	General Information
5	General Stability
34	Inhibitors
10	Ki Value [mM]
18	KM Value [mM]
23	Metals/Ions
5	Molecular Weight [Da]
22	Natural Substrates/ Products (Substrates)
946	Organism
3	PDB ID
9	pH Optimum
1	pH Range
3	pH Stability
2	pI Value
26	Protein Variants
10	Purification (Commentary)
3	Reaction
6	Reaction Type
40	Reference
5	Specific Activity [micromol/min/mg]
3	Storage Stability
59	Substrates and Products (Substrate)
1	Subunits
46	Synonyms
1	Systematic Name
10	Temperature Optimum [°C]
1	Temperature Range [°C]

Purification

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Purification on EC 2.7.7.42 - [glutamine synthetase] adenylyltransferase

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2 entries

2200fold to homogeneity

Escherichia coli

643334

300fold to homogeneity

Escherichia coli

643329

ammonium sulfate fractionation, DE-52 column chromatography, Bio-Gel A0.5M gel filtration, and phenyl Sepharose column chromatography

Escherichia coli

672242

ATase is cloned into the pET101/D-TOPO plasmid, containing ATase fused to a C-terminal V5 epitope followed by six histidines, His-tagged proteins are applied to HisTrap HP 1ml column

Rhodospirillum rubrum

673590

partial

3 entries