3'–5'-exonuclease, ABO4/POL2a/TIL1, Afu polymerase, ASFV DNA polymerase, ASFV Pol X, B-family replicative DNA polymerase, beta type DNA polymerase, Bst DNA polymerase, CpDNApolI, DBH, Dbh DNA polymerase, Dbh polymerase, ddNTP-sensitive DNA polymerase, Deep Vent DNA polymerase, DeepVent DNA polymerase, deoxynucleate polymerase, deoxyribonucleate nucleotidyltransferase, deoxyribonucleic acid duplicase, deoxyribonucleic acid polymerase, deoxyribonucleic duplicase, deoxyribonucleic polymerase, deoxyribonucleic polymerase I, DinB DNA polymerase, DinB homologue, Dmpol zeta, DNA deoxynucleotidyltransferase, DNA duplicase, DNA nucleotidyltransferase, DNA nucleotidyltransferase (DNA-directed), DNA pol, DNA pol B1, DNA Pol eta, DNA Pol lambda, DNA pol NI, DNA pol Y1, DNA polmerase beta, DNA polymerase, DNA polymerase 1, DNA polymerase 2, DNA polymerase 4, DNA polymerase A, DNA polymerase alpha, DNA polymerase B, DNA polymerase B1, DNA polymerase B2, DNA polymerase B3, DNA polymerase beta, DNA polymerase D, DNA polymerase Dbh, DNA polymerase delta, DNA polymerase Dpo4, DNA polymerase epsilon, DNA polymerase eta, DNA polymerase gamma, DNA polymerase I, DNA polymerase II, DNA polymerase III, DNA polymerase III epsilon subunit, DNA polymerase iota, DNA polymerase IV, DNA polymerase kappa, DNA polymerase lambda, DNA polymerase mu, DNA polymerase ny, DNA polymerase pyrococcus kodakaraensis, DNA polymerase theta, DNA polymerase V, DNA polymerase X, DNA polymerase zeta, DNA polymerases B, DNA polymerases D, DNA polymmerase I, DNA primase-polymerase, DNA replicase, DNA replication polymerase, DNA-dependent DNA polymerase, DNAP, DP1Pho, DP2Pho, Dpo1, Dpo2, Dpo3, Dpo4, Dpo4 polymerase, Dpo4-like enzyme, duplicase, error-prone DNA polymerase, error-prone DNA polymerase X, family B-type DNA polymerase, hoPolD, HSV 1 POL, Igni_0062, K4 polymerase, K4pol, K4PolI, kDNA replication protein, KDO XL DNA polymerase, KF(exo-), KF-, Klenow fragment, Klenow-like DNA polymerase I, KOD DNA polymerases, lesion-bypass DNA polymerase, M1 DNA polymerase, M1pol, MacDinB-1, MA_4027, Miranda pol beta protein, mitochondrial DNA polymerase, Mka polB, More, MsDpo4, mtDNA polymerase NI, mtDNA replicase, Neq DNA polymerase, non-replicative DNA polymerase III, nucleotidyltransferase, deoxyribonucleate, OsPOLP1, PabPol D, PabpolB, PabpolD, Pfu, Pfu DNA polymerase, Pfu Pol, Pfu-POl, PH0121, PH0123, phi29 DNA polymerase, phi29 DNApol, PhoPolD, phPol D, Pol, pol alpha, Pol B, Pol B1, pol beta, Pol BI, pol delta, pol E, POl epsilon, Pol eta, Pol gamma, Pol I, Pol II, pol III, pol iota, Pol IV, pol kappa, pol kappaDELTAC, Pol lambda, Pol mu, pol NI, Pol ny, Pol theta, Pol V, pol Vent (exo-), Pol X, Pol zeta, Pol-beta, POL1, Pol2, POL2a, Pol3, Pol31, PolB, POlB1, polbeta, polD, POLD4, Poldelta, POLdelta1, PolDPho, Polepsilon, Poleta, POLG, PolH, polI, POLIB, POLIC, POLID, poliota, Polkappa, PolX, PolY, poly iota, polymerase alpha catalytic subunit A, polymerase III, pORF30, Pwo DNA polymerase, R2 polymerase, R2 reverse transcriptase, R2-RT, RAD30, RB69 DdDp, RB69 DNA Polymerase, RB69pol, Rec1, repair polymerase, replicative DNA polymerase, reverse transcriptase, RKOD DNA polymerase, Rv1537, Rv3056, Saci_0554, sequenase, Sso, Sso DNA pol B1, Sso DNA pol Y1, Sso DNA polymerase Y1, Sso DNApol, Sso pol B1, SSO0552, SSO2448, SsoDpo1, SsoPolB1, SsoPolY, Szi DNA polymerase, T4 DNA polymerase, T7 DNA polymerase, Taq DNA polymerase, Taq Pol I, Taq polymerase, Tba5 DNA polymerase, Tca DNA polymerase, Tga PolB, TGAM_RS07365, Tkod-Pol, translesion DNA polymerase, translesion DNA synthesis polymerase, translesion polymerase Dpo4, UL30/UL42, UmuD'2C, UmuD'2C-RecA-ATP, Vent polymerase, X family DANN polymerase, Y-family DNA polymerase eta
the enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq. Such properties make the Dpo4-like enzymes ideally suited for the polymerase chain reaction amplification of damaged DNA samples. By using a blend of Taq and Dpo4-like enzymes a polymerase chain amplicon is obtained from ultraviolet-irradiated DNA that is largely unamplifyable with Taq alone. The inclusion of thermostable Dpo4-like polymerases in polymerase chain reactions, augments the recovery and analysis of lesion-containing DNA samples, such as those commonly found in forensic or ancient DNA molecular applications
the enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq. Such properties make the Dpo4-like enzymes ideally suited for the polymerase chain reaction amplification of damaged DNA samples. By using a blend of Taq and Dpo4-like enzymes a polymerase chain amplicon is obtained from ultraviolet-irradiated DNA that is largely unamplifyable with Taq alone. The inclusion of thermostable Dpo4-like polymerases in polymerase chain reactions, augments the recovery and analysis of lesion-containing DNA samples, such as those commonly found in forensic or ancient DNA molecular applications
the enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq. Such properties make the Dpo4-like enzymes ideally suited for the polymerase chain reaction amplification of damaged DNA samples. By using a blend of Taq and Dpo4-like enzymes a polymerase chain amplicon is obtained from ultraviolet-irradiated DNA that is largely unamplifyable with Taq alone. The inclusion of thermostable Dpo4-like polymerases in polymerase chain reactions, augments the recovery and analysis of lesion-containing DNA samples, such as those commonly found in forensic or ancient DNA molecular applications
the enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq. Such properties make the Dpo4-like enzymes ideally suited for the polymerase chain reaction amplification of damaged DNA samples. By using a blend of Taq and Dpo4-like enzymes a polymerase chain amplicon is obtained from ultraviolet-irradiated DNA that is largely unamplifyable with Taq alone. The inclusion of thermostable Dpo4-like polymerases in polymerase chain reactions, augments the recovery and analysis of lesion-containing DNA samples, such as those commonly found in forensic or ancient DNA molecular applications
the enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq. Such properties make the Dpo4-like enzymes ideally suited for the polymerase chain reaction amplification of damaged DNA samples. By using a blend of Taq and Dpo4-like enzymes a polymerase chain amplicon is obtained from ultraviolet-irradiated DNA that is largely unamplifyable with Taq alone. The inclusion of thermostable Dpo4-like polymerases in polymerase chain reactions, augments the recovery and analysis of lesion-containing DNA samples, such as those commonly found in forensic or ancient DNA molecular applications
short-patch compartmentalized self-replication will be a powerful strategy for the generation of polymerases with altered substrate specificity for applications in nano- and biotechnology and in the enzymatic synthesis of antisense and RNAi probes
DNA polymerase plays prominent roles in numerous biotechnologies. The use of diphosphate substrates has the potential to make practical the incorporation of expensive analogs, such as isotopically labeled or chemically modified nucleotides, eliminating the need for challenging triphosphate syntheses. This feature of DNA polymerases may also provide a method for detecting nucleotides used in high-throughput DNA sequencing
DNA polymerase plays prominent roles in numerous biotechnologies. The use of diphosphate substrates has the potential to make practical the incorporation of expensive analogs, such as isotopically labeled or chemically modified nucleotides, eliminating the need for challenging triphosphate syntheses. This feature of DNA polymerases may also provide a method for detecting nucleotides used in high-throughput DNA sequencing
DNA polymerase plays prominent roles in numerous biotechnologies. The use of diphosphate substrates has the potential to make practical the incorporation of expensive analogs, such as isotopically labeled or chemically modified nucleotides, eliminating the need for challenging triphosphate syntheses. This feature of DNA polymerases may also provide a method for detecting nucleotides used in high-throughput DNA sequencing
DNA polymerase plays prominent roles in numerous biotechnologies. The use of diphosphate substrates has the potential to make practical the incorporation of expensive analogs, such as isotopically labeled or chemically modified nucleotides, eliminating the need for challenging triphosphate syntheses. This feature of DNA polymerases may also provide a method for detecting nucleotides used in high-throughput DNA sequencing
DNA polymerase plays prominent roles in numerous biotechnologies. The use of diphosphate substrates has the potential to make practical the incorporation of expensive analogs, such as isotopically labeled or chemically modified nucleotides, eliminating the need for challenging triphosphate syntheses. This feature of DNA polymerases may also provide a method for detecting nucleotides used in high-throughput DNA sequencing
DNA polymerase plays prominent roles in numerous biotechnologies. The use of diphosphate substrates has the potential to make practical the incorporation of expensive analogs, such as isotopically labeled or chemically modified nucleotides, eliminating the need for challenging triphosphate syntheses. This feature of DNA polymerases may also provide a method for detecting nucleotides used in high-throughput DNA sequencing
rational design approach to creating modulated proofreading DNA polymerases which can be utilized in a highly sensitive long RT/PCR amenable to the clinical diagnostic setting
rational design approach to creating modulated proofreading DNA polymerases which can be utilized in a highly sensitive long RT/PCR amenable to the clinical diagnostic setting
a mutated thermostable DNA polymerase, Taq M1, from Thermus aquaticus, that exhibits an increased reverse transcriptase activity, is therefore designated for one-step PCR pathogen detection using established real-time detection methods
rational design approach to creating modulated proofreading DNA polymerases which can be utilized in a highly sensitive long RT/PCR amenable to the clinical diagnostic setting
clinically, POLG mutations can present from early neonatal life to late middle age, with a spectrum of phenotypes that includes common neurological disorders such as migraine, epilepsy and Parkinsonism
mitochondrial toxicity is a limiting factor in the use of some nucleoside reverse transcriptase inhibitors of HIV. DNA polymerase gamma incorporates chain-terminating dioxolane guanosine and 2',3'-dideoxy-2',3'-didehydroguanosine more than 3000fold efficiently than the carboxylic analog carbovir triphosphate
mutation R964C is identified in a patient with lactic acidosis. Recombinant R964C Pol gamma shows only 14% activity of wild-type enzyme. The mutation could be associated with the severe lactic acidosis induced by long-term use of nucleoside reverse-transcriptase inhibitors
generation of a unique one enzyme system with high fidelity to allow highly accurate and efficient amplification of DNA targets using polymerase chain reaction by fusing Sso7d protein to Tpa DNA polymerase
the high fidelty of the enzyme is suitable fo polymerase chain reaction (PCR), which requires accurate DNA amplification for gene cloning and diagnostic assay
long and accurate PCR can be achieved with a mixture of wild type DNA polymerase from Thermococcus kodakaraensis and its exonuclease deficient mutant enzyme N210D is utilized (at the ratio of 1:40)
optimal conditions for polymerase chain reaction are determined. Iho DNA polymerase possesses 3'->5' exonuclease activity, and the fidelity of the Iho DNA polymerase is similar to that of Pfu and Vent DNA polymerases. However, Iho DNA polymerase provides more enhanced efficiency of PCR amplification than Pfu and Vent DNA polymerases. Iho DNA polymerase can successfully amplify a 2-kb lambda DNA target with a 10/s extension time and could amplify a DNA fragment up to 8 kb lambda DNA
application for long and accurate PCR. The PCR error rate of the Tba5 DNA polymerase plus4 (Tba5 plus DNA polymerase mixtures are constituted with various amounts of Tba5 DNA polymerase mixed with Taq DNA polymerase) is much lower than that of the wild-type enzyme alone
the thermostable properties of the enzyme from Thermus aquaticus have contributed majorly to the specificity, automation, and efficacy of the polymerase chain reaction (PCR)
optimal conditions for polymerase chain reaction are determined. Iho DNA polymerase possesses 3'->5' exonuclease activity, and the fidelity of the Iho DNA polymerase is similar to that of Pfu and Vent DNA polymerases. However, Iho DNA polymerase provides more enhanced efficiency of PCR amplification than Pfu and Vent DNA polymerases. Iho DNA polymerase can successfully amplify a 2-kb lambda DNA target with a 10/s extension time and could amplify a DNA fragment up to 8 kb lambda DNA
generation of a unique one enzyme system with high fidelity to allow highly accurate and efficient amplification of DNA targets using polymerase chain reaction by fusing Sso7d protein to Tpa DNA polymerase
the high fidelty of the enzyme is suitable fo polymerase chain reaction (PCR), which requires accurate DNA amplification for gene cloning and diagnostic assay
application for long and accurate PCR. The PCR error rate of the Tba5 DNA polymerase plus4 (Tba5 plus DNA polymerase mixtures are constituted with various amounts of Tba5 DNA polymerase mixed with Taq DNA polymerase) is much lower than that of the wild-type enzyme alone
the enzyme is useful in DNA amplification and PCR-based applications, particularly in clinical diagnoses using uracil-DNA glycosylase. A mixture of Nanoarchaeum equitans DNA polymerase and Thermus aquaticus DNA polymerase improves the performance of Neq DNA polymerase for long and accurate PCR
the very good competition of 7-substituted 7-deazapurine dNTPs, and still reasonably good activity of 5-substituted pyrimidine dNTPs, in the presence of their natural counterparts is very encouraging for further development of methods of polymerase synthesis of modified DNA and for possible in cellulo and even in vivo applications if satisfactory delivery of modified dNTPs will be solved
the very good competition of 7-substituted 7-deazapurine dNTPs, and still reasonably good activity of 5-substituted pyrimidine dNTPs, in the presence of their natural counterparts is very encouraging for further development of methods of polymerase synthesis of modified DNA and for possible in cellulo and even in vivo applications if satisfactory delivery of modified dNTPs will be solved
the very good competition of 7-substituted 7-deazapurine dNTPs, and still reasonably good activity of 5-substituted pyrimidine dNTPs, in the presence of their natural counterparts is very encouraging for further development of methods of polymerase synthesis of modified DNA and for possible in cellulo and even in vivo applications if satisfactory delivery of modified dNTPs will be solved
the very good competition of 7-substituted 7-deazapurine dNTPs, and still reasonably good activity of 5-substituted pyrimidine dNTPs, in the presence of their natural counterparts is very encouraging for further development of methods of polymerase synthesis of modified DNA and for possible in cellulo and even in vivo applications if satisfactory delivery of modified dNTPs will be solved