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1.1.1.71: alcohol dehydrogenase [NAD(P)+]

This is an abbreviated version!
For detailed information about alcohol dehydrogenase [NAD(P)+], go to the full flat file.

Wordmap for 1.1.1.71

Word Map on EC 1.1.1.71 Wordmap for 1.1.1.71

Reaction

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a primary alcohol
+
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NAD(P)+
=
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an aldehyde
 +
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NAD(P)H
 +
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H+

Synonyms

ADH, ADH12, ADH2, Adh319, AdhA, AdhE, alcohol dehydrogenase, aldehyde reductase (NADPH/NADH), DHRS3, HvADH2, HVO_B0071, NAD(P)+-dependent alcohol dehydrogenase, NAD(P)H-dependent ADH, NAD(P)H-dependent aldehyde reductase, NADPH-dependent ADHA, NADPH-dependent alcohol dehydrogenase, PH0743, PhADH, RADH, retinal reductase, retinal short-chain dehydrogenase/reductase member 3, retinaldehyde reductase, Retinol dehydrogenase, retinol-active alcohol dehydrogenase, retSDR1, TeSADH, TsAdh319, Tsib_0319, VNG_2617G, YqhD

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                EC 1.1.1.711.1.1.71 alcohol dehydrogenase [NAD(P)+]

Purification

Purification on EC 1.1.1.71 - alcohol dehydrogenase [NAD(P)+]

for references in articles please use BRENDA:EC1.1.1.71

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, DEAE-cellulose, Sephadex G-100
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ammonium sulfate, DEAE-Sephadex A-50, hydroxyapatite, Sephadex G-200
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ammonium sulfate, DEAE-Sephadex, Sephadex G-200, 55°C for 6 min
-
chromatofocusing, reactive red
-
DEAE-Sephacel, ammonium sulfate, Sephacryl S300HR, Phenyl-Sepharose, Red A
-
DEAE-Sephacel, CM-cellulose, Sephacryl S300HR, Phenyl-Sepharose, Blue A/DEAE-Sephacel
-
HisTrap column chromatography, and Superdex 200 gel filtration
-
isolation of a gene, that is induced after culture filtrate treatment from Erwinia carotovora
Ni-NTA column chromatography and Superdex 200 pg gel filtration
purified in one step by immobilised Ni-affinity chromatography
purified in one step by immobilized metal-affinity chromatography
Q sepharose column chromatography
-
Q Sepharose column chromatography, and Superdex 200 gel filtration
-
recombinant StrepII-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography, ultrafiltration, and gel filtration
strain B592, DEAE-cellulose, hydroxyapatite, Sephacryl S-300, Matrex Gel Red A
-