1.11.1.5: cytochrome-c peroxidase

This is an abbreviated version!
For detailed information about cytochrome-c peroxidase, go to the full flat file.

Word Map on EC 1.11.1.5

Reaction

2 ferrocytochrome c +

H2O2
=
2 ferricytochrome c
+ 2 H2O

Synonyms

apocytochrome c peroxidase, BCcP, CCP, CCP1, CcpA, Cjj0382, CytC, cytochrome c iso-1, cytochrome c peroxidase, cytochrome c-551 peroxidase, cytochrome c-H2O oxidoreductase, cytochrome peroxidase, di-heme cytochrome c peroxidase, diheme cytochrome c peroxidase, diheme cytochrome c5 peroxidase CcpA, DocA, LmP, MacA, mesocytochrome c peroxidase azide, mesocytochrome c peroxidase cyanate, mesocytochrome c peroxidase cyanide, peroxidase, cytochrome c, Psa CcP

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.5 cytochrome-c peroxidase

Purification

Purification on EC 1.11.1.5 - cytochrome-c peroxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
four-step purification protocol
-
gel filtration and ion exchange chromatography
-
high-purity V197C/C128A mutant is obtained after an anion-exchange chromatography and gel filtration
-
inner membrane proteins solubilized in sarkosyl solution and precipitated with ethanol are purified by affinity chromatography
-
native enzyme partially by preparation of mitochondria
-
partially purified
-
precipitation, DEAE-Sepharose followed by S-100 size exclusion chromatography
purified to homogeneity
-
purified to homogeneity in three steps
-
recombinant His-tagged CcpA from Escherichia coli strain AS457
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant N-terminally Strep-tagged protein from Escherichia coli strain BL21(DE3) by streptactin affinity chromatography and gel filtration
recombinant soluble His-tagged Ccp from Escherichia coli strain BL21(DE3) by immobilized metal affinity chromatography and gel filtration
-
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by anion exchange chromatography and dialysis
-
S-Sepharose column chromatography and Superdex-75 gel filtration