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1.13.11.85: exo-cleaving rubber dioxygenase

This is an abbreviated version!
For detailed information about exo-cleaving rubber dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.85

Reaction

cis-1,4-polyisoprene
+ n O2 = n (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal

Synonyms

heme-dependent rubber oxygenase, LATA, poly(cis-1,4-isoprene)-cleavage enzyme, roxA, RoxB, RubA, rubber oxygenase, rubber oxygenase A

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.85 exo-cleaving rubber dioxygenase

Application

Application on EC 1.13.11.85 - exo-cleaving rubber dioxygenase

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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
development of an in vitro enzyme assay for oxidative rubber degradation based on high-performance liquid chromatography analysis and spectroscopic detection of product carbonyl functions after derivatization with dinitrophenylhydrazone
synthesis
optimization of a transformation system via electroporation, and a conjugation system for expression of RoxA in Xanthomonas sp. About 6 mg purified RoxA are obtained from 1 l of cell-free culture fluid