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1.13.12.24: calcium-regulated photoprotein

This is an abbreviated version!
For detailed information about calcium-regulated photoprotein, go to the full flat file.

Word Map on EC 1.13.12.24

Reaction

[aequorin]
+ 3 Ca2+ =
[aequorin] 1,2-dioxetan-3-one

Synonyms

aequorin, alcium-activated photoprotein, berovin, BFP-aq, blue fluorescent protein, Ca2+-binding photoprotein, Ca2+-regulated photoprotein, clytin, halistaurin, mitrocomin, mnemiopsin, mnemiopsin 1, mnemiopsin 2, mnemiopsin1, obelin, phialidin

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
                1.13.12.24 calcium-regulated photoprotein

Activating Compound

Activating Compound on EC 1.13.12.24 - calcium-regulated photoprotein

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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
reuired. About 1% residual activity in absence of 2-mercaptoethanol
dithiothreitol
in the presence of DTT, continuous luminescence is observed over 1 h. In the absence of DTT, the luminescence activity slowly decreases with a half period of 8.3 min
imidazole
luminescence activity of BFP-aq 1s stimulated 1.5- to 2fold by imidazole at concentrations of 30-300 mM. Imidazole has no effect on the binding affinity of coelenterazine and may act as a catalytic base
additional information
1H-pyrazole, 2-methylimidazole, 4-methylimidazole, histamine, L-histidine, urocanic acid, and 5-amino-4-imidazole carboxamide do not act as stimulators at concentrations of 30 and 150 mM
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