1.13.12.24: calcium-regulated photoprotein
This is an abbreviated version!
For detailed information about calcium-regulated photoprotein, go to the full flat file.
Word Map on EC 1.13.12.24
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1.13.12.24
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photoproteins
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bioluminescence
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aequorin
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luminescence
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obelia
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longissima
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coelenteramide
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ctenophore
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coelenterate
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jellyfish
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ef-hand
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2-hydroperoxycoelenterazine
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mnemiopsis
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hydroid
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clytin
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hydromedusan
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leidyi
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synthesis
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analysis
- 1.13.12.24
-
photoproteins
-
bioluminescence
- aequorin
-
luminescence
- obelia
- longissima
- coelenteramide
-
ctenophore
-
coelenterate
-
jellyfish
-
ef-hand
- 2-hydroperoxycoelenterazine
- mnemiopsis
- hydroid
- clytin
-
hydromedusan
- leidyi
- synthesis
- analysis
Reaction
Synonyms
aequorin, alcium-activated photoprotein, berovin, BFP-aq, blue fluorescent protein, Ca2+-binding photoprotein, Ca2+-regulated photoprotein, clytin, halistaurin, mitrocomin, mnemiopsin, mnemiopsin 1, mnemiopsin 2, mnemiopsin1, obelin, phialidin
ECTree
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Activating Compound
Activating Compound on EC 1.13.12.24 - calcium-regulated photoprotein
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2-mercaptoethanol
reuired. About 1% residual activity in absence of 2-mercaptoethanol
dithiothreitol
in the presence of DTT, continuous luminescence is observed over 1 h. In the absence of DTT, the luminescence activity slowly decreases with a half period of 8.3 min
imidazole
luminescence activity of BFP-aq 1s stimulated 1.5- to 2fold by imidazole at concentrations of 30-300 mM. Imidazole has no effect on the binding affinity of coelenterazine and may act as a catalytic base
additional information
1H-pyrazole, 2-methylimidazole, 4-methylimidazole, histamine, L-histidine, urocanic acid, and 5-amino-4-imidazole carboxamide do not act as stimulators at concentrations of 30 and 150 mM
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