1.14.12.25: p-cumate 2,3-dioxygenase

This is an abbreviated version!
For detailed information about p-cumate 2,3-dioxygenase, go to the full flat file.

Reaction

p-cumate
+
NADH
+
H+
+
O2
=
(2R,3S)-2,3-dihydroxy-2,3-dihydro-p-cumate
+
NAD+

Synonyms

CmtAa, CmtAb, CmtAc, CmtAd, p-cumate dioxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
                1.14.12.25 p-cumate 2,3-dioxygenase

Engineering

Engineering on EC 1.14.12.25 - p-cumate 2,3-dioxygenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
enrichment of mutants defective in the utilization of p-cymene as sole carbon source by incubation of cells in minimal growth media containing p-cymene, p-chlorotoluene, p-bromotoluene, alpha-chloro-p-xylene, or p-iodobenzoate. Several classes of mutants can be isolated, p-cymene hydroxylase and p-cumate 2,3-dioxygenase are likely to be multicomponent enzymes