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1.3.8.12: (2S)-methylsuccinyl-CoA dehydrogenase

This is an abbreviated version!
For detailed information about (2S)-methylsuccinyl-CoA dehydrogenase, go to the full flat file.

Reaction

(2S)-methylsuccinyl-CoA
+
electron-transfer flavoprotein
=
2-methylfumaryl-CoA
+
reduced electron-transfer flavoprotein

Synonyms

MCD, methylsuccinyl-CoA dehydrogenase, PdMCD

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.8 With a flavin as acceptor
                1.3.8.12 (2S)-methylsuccinyl-CoA dehydrogenase

Crystallization

Crystallization on EC 1.3.8.12 - (2S)-methylsuccinyl-CoA dehydrogenase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant FAD-bound enzyme, sitting drop vapor diffusion method, mixing of 20 mg/ml protein in 20 mM Tris-HCl, pH 7.9, 200 mM NaCl, 1.5 mM FAD, and 3 mM mesaconyl-CoA in an 1:1 ratio with crystallization solution containing 30% PEG 5000 monomethyl ether mesylate, 100 mM Tris, pH 8.0, and 200 mM LiSO4, 16°C, X-ray diffraction structure determination and analysis at 1.37 A resolution, modeling
sitting drop vapor diffusion method, using 30% (w/v) PEG 5000 monomethyl ether mesylate, 100 mM Tris, pH 8.0, and 200 mM LiSO4