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0.098
(3R,3'R)-dimethylspermine
-
pH 7.4, 25°C
-
0.061
(3S,3'S)-dimethylspermine
-
pH 7.4, 25°C
-
1.6
benzylamidine
-
pH 8.5, 37°C
1.898
N,N'-bis(3-aminopropyl)ethylenediamine
-
pH 7.2, 37°C
0.027 - 0.37
N,N'-dibenzyl-1,4-diaminobutane
0.042 - 1
N1-acetylspermidine
0.0019 - 2.125
N1-acetylspermine
5
norspermidine
-
pH 8.5, 37°C
0.0037 - 0.05
thermospermine
2.5
tryptamine
-
pH 8.5, 37°C
additional information
additional information
-
0.027
N,N'-dibenzyl-1,4-diaminobutane
-
pH 8.6, 30°C, recombinant wild-type enzyme
0.13
N,N'-dibenzyl-1,4-diaminobutane
-
pH 8.6, 30°C, recombinant mutant H64Q
0.27
N,N'-dibenzyl-1,4-diaminobutane
-
pH 8.6, 30°C, recombinant mutant H64A
0.37
N,N'-dibenzyl-1,4-diaminobutane
-
pH 8.6, 30°C, recombinant mutant H64N
0.042
N1-acetylspermidine
pH 7.5, 37°C, recombinant AtPAO3
1
N1-acetylspermidine
37°C, pH 7.0
0.0019
N1-acetylspermine
37°C, pH 7.5
0.011
N1-acetylspermine
pH 9.0, 25°C, recombinant wild-type Fms1
0.02
N1-acetylspermine
pH 7.5, 37°C, recombinant AtPAO3
0.0218
N1-acetylspermine
37°C, pH 6.5
0.03
N1-acetylspermine
pH 9.0, 25°C, recombinant mutant D94N
0.0359
N1-acetylspermine
-
pH 8.5, 30°C
0.0369
N1-acetylspermine
37°C, pH 8.0, wild-type enzyme
0.043
N1-acetylspermine
37°C, pH 8.0, mutant enzyme K315M
0.046
N1-acetylspermine
37°C, pH 8.0, mutant enzyme T467Y
0.05
N1-acetylspermine
-
pH 10.0, 30°C, recombinant mutant H64A
0.053
N1-acetylspermine
-
pH 10.0, 30°C, recombinant mutant H64Q
0.058
N1-acetylspermine
37°C, pH 8.0, mutant enzyme T467S
0.062
N1-acetylspermine
37°C, pH 8.0, mutant enzyme H69E
0.071
N1-acetylspermine
37°C, pH 8.0, mutant enzyme H69Q
0.104
N1-acetylspermine
pH 9.0, 25°C, recombinant mutant N195A
0.21
N1-acetylspermine
-
pH 10.0, 30°C, recombinant wild-type enzyme
0.233
N1-acetylspermine
pH 7.5, temperature not specified in the publication
0.233
N1-acetylspermine
pH 7.5, 25°C, recombinant AtPAO2
0.24
N1-acetylspermine
-
pH 10.0, 30°C, recombinant mutant H64N
2
N1-acetylspermine
37°C, pH 7.0
2
N1-acetylspermine
pH 7.5, temperature not specified in the publication
2
N1-acetylspermine
pH 7.5, 25°C, recombinant AtPAO3
2.125
N1-acetylspermine
-
pH 7.2, 37°C
0.011
norspermine
-
pH 6.0, 30°C
0.0145
norspermine
-
pH 8.5, 30°C
0.0158
norspermine
37°C, pH 6.5
0.0255
norspermine
37°C, pH 7.5
0.045
norspermine
pH 7.5, temperature not specified in the publication
0.045
norspermine
pH 7.5, 25°C, recombinant AtPAO3
2
norspermine
-
pH 8.5, 37°C
0.0046
O2
pH 7.5, 25°C, cosubstrate: N1-acetylspermine
0.0051
O2
pH 7.5, 25°C, cosubstrate: thermospermine
0.015
O2
pH 9.0, 25°C, recombinant mutant N195A, with spermine
0.02
O2
pH 9.0, 25°C, recombinant mutant N195A, with N1-acetylspermine
0.0436
O2
pH 9.0, 25°C, recombinant wild-type Fms1, with N1-acetylspermine
0.07
O2
-
pH 10.0, 30°C, recombinant wild-type enzyme, with spermine
0.091
O2
pH 9.0, 25°C, recombinant wild-type Fms1, with spermine
0.097
O2
pH 9.0, 25°C, recombinant mutant D94N, with N1-acetylspermine
0.15
O2
-
pH 8.6, 30°C, recombinant mutants H64A and H64Q, with N,N'-dibenzyl-1,4-diaminobutane
0.16
O2
-
pH 8.6, 30°C, recombinant wild-type enzyme, with N,N'-dibenzyl-1,4-diaminobutane
0.192
O2
pH 9.0, 25°C, recombinant mutant D94N, with spermine
0.2
O2
-
pH 8.6, 30°C, recombinant mutant H64N, with N,N'-dibenzyl-1,4-diaminobutane
0.3
O2
-
pH 10.0, 30°C, recombinant mutants H64A and H64N, with spermine
0.5
O2
-
pH 10.0, 30°C, recombinant mutant H64Q, with spermine
11
O2
pH 7.5, 25°C, cosubstrate: spermine
39
O2
-
pH 10.0, 30°C, recombinant wild-type enzyme and recombinant mutants H64A and H64N, with N1-acetylspermine
53
O2
-
pH 10.0, 30°C, recombinant mutant H64Q, with N1-acetylspermine
0.007
spermidine
37°C, pH 9.5, wild-type enzyme
0.0124
spermidine
37°C, pH 9.5, wild-type enzyme
0.016
spermidine
37°C, pH 9.5, mutant enzyme K301M
0.017
spermidine
37°C, pH 9.5, mutant enzyme T460Y
0.019
spermidine
37°C, pH 9.5, mutant enzyme H64E
0.019
spermidine
37°C, pH 9.5, mutant enzyme T460S
0.02
spermidine
37°C, pH 9.5, mutant enzyme T467Y
0.021
spermidine
37°C, pH 9.5, mutant enzyme H64Q
0.0229
spermidine
37°C, pH 9.5, mutant enzyme H69Q
0.025
spermidine
37°C, pH 9.5, mutant enzyme H69E
0.031
spermidine
37°C, pH 9.5, mutant enzyme K315M
0.0683
spermidine
37°C, pH 7.5
0.139
spermidine
pH 7.5, 25°C, recombinant AtPAO4
0.182
spermidine
37°C, pH 9.5, mutant enzyme T467S
0.204
spermidine
37°C, pH 7.0
0.204
spermidine
pH 7.5, 37°C, recombinant AtPAO3
0.274
spermidine
pH 7.5, temperature not specified in the publication
0.274
spermidine
pH 7.5, 25°C, recombinant AtPAO3
0.409
spermidine
pH 7.5, temperature not specified in the publication
0.409
spermidine
pH 7.5, 25°C, recombinant AtPAO2
0.456
spermidine
pH 8.5, 30°C
0.66
spermidine
-
pH 8.5, 37°C
2.34
spermidine
-
pH 7.2, 37°C
0.0049
spermine
-
pH 6.0, 30°C
0.0061
spermine
-
pH 8.5, 30°C
0.00836
spermine
37°C, pH 8.5, wild-type enzyme
0.012
spermine
37°C, pH 8.5, mutant enzyme T460S
0.013
spermine
37°C, pH 8.5, mutant enzyme T460Y
0.017
spermine
37°C, pH 8.5, mutant enzyme K301M
0.024
spermine
37°C, pH 8.5, mutant enzyme H64E
0.0256
spermine
37°C, pH 7.5
0.0269
spermine
37°C, pH 8.5, mutant enzyme H64Q
0.04
spermine
-
pH 10.0, 30°C, recombinant mutant H64N
0.047
spermine
pH 7.5, 25°C, recombinant AtPAO4
0.077
spermine
-
pH 7.4, 25°C
0.0787
spermine
37°C, pH 6.5
0.098
spermine
pH 8.5, 30°C
0.118
spermine
pH 9.35, 25°C, recombinant wild-type Fms1
0.127
spermine
pH 9.35, 25°C, recombinant mutant N195A
0.16
spermine
-
pH 10.0, 30°C, recombinant wild-type enzyme
0.21
spermine
-
pH 10.0, 30°C, recombinant mutants H64A and H64Q
0.211
spermine
-
pH 7.2, 37°C
0.27
spermine
pH 7.5, temperature not specified in the publication
0.27
spermine
pH 7.5, 25°C, recombinant AtPAO2
0.32
spermine
pH 9.35, 25°C, recombinant mutant D94N
0.35
spermine
-
pH 8.5, 37°C
0.58
spermine
pH 7.5, temperature not specified in the publication
0.58
spermine
pH 7.5, 25°C, recombinant AtPAO3
0.588
spermine
37°C, pH 7.0
0.588
spermine
pH 7.5, 37°C, recombinant AtPAO3
0.0037
thermospermine
-
pH 6.0, 30°C
0.0051
thermospermine
37°C, pH 7.5
0.0137
thermospermine
37°C, pH 6.5
0.05
thermospermine
pH 7.5, temperature not specified in the publication
0.05
thermospermine
pH 7.5, 25°C, recombinant AtPAO3
additional information
additional information
-
steady-state kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
seady-state kinetics and rapid-reaction kinetics of wild-type and mutant enzymes, overview
-
additional information
additional information
steady-state kinetics and rapid-reaction kinetics of wild-type and mutant enzymes, overview
-