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2.4.2.59: sulfide-dependent adenosine diphosphate thiazole synthase

This is an abbreviated version!
For detailed information about sulfide-dependent adenosine diphosphate thiazole synthase, go to the full flat file.

Reaction

NAD+
+
glycine
+
Sulfide
=
nicotinamide
+
ADP-5-ethyl-4-methylthiazole-2-carboxylate
+ 3 H2O

Synonyms

MJ0601, Thi4, thiamine thiazole synthase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.59 sulfide-dependent adenosine diphosphate thiazole synthase

Crystallization

Crystallization on EC 2.4.2.59 - sulfide-dependent adenosine diphosphate thiazole synthase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme complexed with ADP-ribulose, hanging drop vapor diffusion method, using 1.05 M potassium/sodium tartrate, 0.1 M CHES/sodium hydroxide, pH 9.5 and 0.2 M lithium sulfate
enzyme complexes with glycine imine intermediate and iron, hanging drop vapor diffusion method, using 1.0 M sodium citrate, 0.1 M CHES/sodium hydroxide, pH 9.5