EC Number   |
Protein Variants |
Reference |
|---|
   1.14.11.51 | C118A/C129A |
about 50% decrease in m6A demethylase activity, about 20% decrease in apurinic/apyrimidinic lyase activity |
764135 |
| 1.14.11.51 | K133A |
about 50% decrease in m6A demethylase activity, about 45% decrease in apurinic/apyrimidinic lyase activity |
764135 |
| 1.14.11.51 | C129A |
about 90% decrease in m6A demethylase activity, about 30% decrease in apurinic/apyrimidinic lyase activity |
764135 |
| 1.14.11.51 | D186A |
inactive |
742235 |
| 1.14.11.51 | C118A |
m6A demethylase activity similar to wild-type, about 25% decrease in apurinic/apyrimidinic lyase activity |
764135 |
| 1.14.11.51 | S235D |
mutation disrupts N6-mA demethylation activity |
764442 |
| 1.14.11.51 | S235E |
mutation disrupts N6-mA demethylation activity |
764442 |
| 1.14.11.51 | R344A |
mutation in highly conserved residue, strong decrease in demethylation ability |
764114 |
| 1.14.11.51 | Y184A |
mutation in highly conserved residue, strong decrease in demethylation ability |
764114 |
| 1.14.11.51 | K158A |
mutation of basic residue of Flip1 region, abolishes N6-mA demethylation activity of ALKBH1 |
764442 |
