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1.17.1.4: xanthine dehydrogenase

This is an abbreviated version!
For detailed information about xanthine dehydrogenase, go to the full flat file.

Word Map on EC 1.17.1.4

Reaction

xanthine
+
NAD+
 +
H2O
=
Urate
 +
NADH
 +
H+

Synonyms

AtXDH1, EC 1.1.1.204, EC 1.2.1.37, IAO1, More, NAD-xanthine dehydrogenase, PaoABC, Retinol dehydrogenase, Rosy locus protein, VvXDH, xanthine dehydrogenase, xanthine dehydrogenase-1, xanthine dehydrogenase-2, xanthine dehydrogenase/oxidase, xanthine oxidoreductase, xanthine-NAD oxidoreductase, xanthine/NAD+ oxidoreductase, xanthine:NAD+ oxidoreductase, XDH, XDH/XO, XDH1, XDH2, XdhC, XOR, YagR, YagS, YagT

ECTree

     1 Oxidoreductases
         1.17 Acting on CH or CH2 groups
             1.17.1 With NAD+ or NADP+ as acceptor
                1.17.1.4 xanthine dehydrogenase

Specific Activity

Specific Activity on EC 1.17.1.4 - xanthine dehydrogenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0011
-
substrate pterin, 22°C, pH 8.3
0.013
-
substrate xanthine, 22°C, pH 8.3
0.044
purified recombinant enzyme, substrate xanthine, pH 8.0
0.06
-
substrate xanthine, pH 7.2, 25°C
0.16
-
xanthine oxidase activity
0.21
-
substrate NAD+, pH 7.2, 25°C
0.23
-
xanthine-NAD+ assay
0.25
-
substrate NAD+, pH 7.2, 25°C
0.29
-
substrate NAD+, pH 7.2, 25°C
0.65
purified recombinant enzyme, pH 8.0, 25°C
0.69
-
substrate xanthine, pH 7.2, 25°C
0.702
purified recombinant enzyme, substrate NADH, pH 6.6
0.9
-
crude extract
1.17
-
pH 8.5
1.56
-
xanthine dehydrogenase activity
1.712
purified recombinant enzyme, in presence of sulfide/dithionite
1.83
-
substrate xanthine, pH 7.2, 25°C
10
-
purified native enzyme, pH and temperature not specified in the publication
163
-
ferricyanide-linked activity
164
-
hypoxanthine-NADP+-assay
17.8
-
pancreas enzyme
2.47
-
-
2.6
-
xanthine-2,6-dichlorophenolindophenol assay
2.8
-
highest activity if growth medium contains urate and is supplemented with 0.0001 mM selenite and 0.0001 mM tungstate
25.2
-
intestine enzyme with allopurinol at 50 mg/kg body weight
26.6
-
intestine enzyme
26.7
-
hypoxanthine-NAD+ assay
29.1
-
purified recombinant enzyme, pH and temperature not specified in the publication
29.5
-
kidney enzyme with allopurinol at 50 mg/kg body weight
3.7
-
highest activity if growth medium contains urate and is supplemented with 0.0001 mM selenite and 0.0001 mM molybdate
35.2
-
after 39.1fold purification
49.9
-
kidney enzyme
6.58
-
pH 7.8, 25°C, expression of xdhAB genes
7
purified recombinant enzyme, pH and temperature not specified in the publication
7.5
-
purified native enzyme, pH and temperature not specified in the publication
72.56
-
xanthine-ferricyanide assay
80
-
pH 7.8, 25°C, expression of xdhABC genes
additional information