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2.4.1.1: glycogen phosphorylase

This is an abbreviated version!
For detailed information about glycogen phosphorylase, go to the full flat file.

Word Map on EC 2.4.1.1

Reaction

[(1->4)-alpha-D-glucosyl]n
+
phosphate
=
[(1->4)-alpha-D-glucosyl]n-1
 +
alpha-D-glucose 1-phosphate

Synonyms

1,4-alpha-glucan phosphorylase, alpha-1,4 glucan phosphorylase, alpha-1,4-glycan phosphorylase, alpha-glucan phosphorylase, alpha-glucan phosphorylase H, alpha-glucan/maltodextrin phosphorylase, alphaGP, amylopectin phosphorylase, amylophosphorylase, CcStP, cyosolic phosphorylase, GlgP, glucan phosphorylase, glucosan phosphorylase, glycogen phosphorylase, glycogen phosphorylase a, glycogen phosphorylase b, glycogen phosphorylase-a, GP, GP b, GPA, GPase, GPase a, GPb, GPBB, GPH, granulose phosphorylase, MalP, maltodextrin phosphorylase, More, muscle glycogen phosphorylase, muscle phosphorylase, muscle phosphorylase a and b, myophosphorylase, PF1535, Phb, PHO, Pho 2, Pho1, phosphorylase a, phosphorylase b, phosphorylase, alpha-glucan, plastidial phosphorylase, polyphosphorylase, potato phosphorylase, RMGPa, rmGPb, SP, starch phosphorylase, starch phosphorylase H, stGP, StP, tGPGG, type L alpha-glucan phosphorylase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.1 glycogen phosphorylase

Cloned

Cloned on EC 2.4.1.1 - glycogen phosphorylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination and analysis
expression in Escherichia coli
expression of His6-tagged Pho1 in Eschericia coli EA3457 cells
expression of Pho1 in Escherichia coli strain DF1020
-
expression of wild-type and mutant enzymes in Escherichia coli
-
expression of wild-type and N-terminal deletion mutant glycogen phosphorylase in Escherichia coli
-
gene glgP, DNA sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
GPase gene, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, expression in Escherichia coli strain DH5alpha
heterologous gene expression of enzyme harboring Strep-Tag II fused to the C-terminal Tyr-465 of the enzyme, in Thermococcus kodakaraensis
-
maltodextrin phosphorylase from Pyrococcus furiosus is fused with the cellulose-binding domain of Clostridium cellulovorans serving as an aggregation module. After molecular cloning of the corresponding gene fusion construct and controlled expression in Escherichia coli BL21, 83% of total maltodextrin phosphorylase activity is displayed in active inclusion bodies
-
ORF Cthe0357, DNA and amino acid sequence determination and analysis, expression of C-terminally His-tagged enzyme in Escherichia coli, expression of cellulose-binding module-intein-alphaGP fusion protein
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overexpression of the muscle isozyme in hepatocytes, causes dissociation of glycogen synthase from proteoglycogen leading to inhibition of initiation of glycogen synthesis
-