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2.4.1.115: anthocyanidin 3-O-glucosyltransferase

This is an abbreviated version!
For detailed information about anthocyanidin 3-O-glucosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.115

Reaction

UDP-D-glucose
+
an anthocyanidin
=
UDP
+
an anthocyanidin-3-O-beta-D-glucoside

Synonyms

3-GT, 3GT, 3GT1, anthocyanidin 3-O-glucosyltransferase, anthocyanidin 3GT, anthocyanidin-3-O-glucosyltransferase, Ct3GT-A, EC 2.4.1.233, FaGT1, glucosyltransferase, uridine diphosphoglucose-anthocyanidin 3-O-, Ih3GT, L3GT, MrUFGT, pMdUFGT, UDP glucose-anthocyanidin 3-O-glucosyltransferase, UDP-glucose: anthocyanidin 3-O-glucosyltransferase, UDP-glucose: flavonoid 3-O-glucosyltransferase, UDP-glucose:anthocyanidin 3-O-beta-D-glucosyltransferase, UDP-glucose:anthocyanidin 3-O-glucosyltransferase, UDP-glucose:anthocyanidin/flavonol 3-O-glucosyltransferase, UDP-glucose:anthocyanin 3-O-glucosyltransferase, UDP-glucose:cyanidin 3-O-glucosyltransferase, UDP-glucose:cyanidin-3-O-glucosyltransferase, UDP-glucose:flavonoid 3-O-glucosyltransferase, UF3GT, UFGT, UGT78K1, UGT78K6

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.115 anthocyanidin 3-O-glucosyltransferase

Purification

Purification on EC 2.4.1.115 - anthocyanidin 3-O-glucosyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
glutathione Sepharose column chromatography
Ni Sepharose column chromatography
partial
-
recombinant
-
recombinant His-tagged enzyme from Escherichia coli strain XL1 Blue by nickel affinity chromatography, removal of the N-terminal His-tag by digestion using recombinant enterokinase, followed by cation exchange chromatography, to homogeneity
Talon metal affinity column chromatography, gel filtration