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2.5.1.29: geranylgeranyl diphosphate synthase

This is an abbreviated version!
For detailed information about geranylgeranyl diphosphate synthase, go to the full flat file.

Word Map on EC 2.5.1.29

Reaction

(2E,6E)-farnesyl diphosphate
+
isopentenyl diphosphate
=
diphosphate
 +
geranylgeranyl diphosphate

Synonyms

At4g36810, bifunctional farnesyl/geranylgeranyl diphosphate synthase, BTS1, CcGGDPS1, CcGGDPS2, CotB1, CrtE, DR1395, EgcrtE, EuFPS1, EuFPS2, farnesyl diphosphate/geranylgeranyl diphosphate synthase, farnesyl-diphosphate/geranylgeranyldiphosphate, farnesyltransferase, FPPS/GGPPS, geranylgeranyl diphosphate synthase, geranylgeranyl diphosphate synthase 1, geranylgeranyl diphosphate synthase 11, geranylgeranyl pyrophosphate synthase, geranylgeranyl pyrophosphate synthase GACE1337, geranylgeranyl pyrophosphate synthase GGPPS1-1, geranylgeranyl pyrophosphate synthase GGPPS1-2, geranylgeranyl pyrophosphate synthetase, geranylgeranyl-PP synthetase, geranylgeranyl-pyrophosphate synthase, GGDP synthase, GGDPS, GGPP synthase, GGPP-S, GGPPase, GGPPS, GGPPS 1, GGPPS 191, GGPPS 2, GGPPS 3, GGPPS 595, GGPPS 727, GGPPS1, GGPPS11, GGPS, Ggps1, GGs1, GgsA, GgsB, GGSP1, gps, HpGGPPS, HsGGPPS, IbGGPS, IdsA, isoprenyl diphosphate synthase, leaf-specific geranylgeranyl pyrophosphate synthase, More, PaxG, PfFPPS, PfGGPPS, Saci_0092, short-chain type-III GGPP, synthetase, geranylgeranyl pyrophosphate, TgFPPS, Tk-IdsA, trans-prenyl diphosphate synthase, type II geranylgeranyl diphosphate synthase, type-III geranylgeranyl pyrophosphate synthase, type-III GGPPS

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.29 geranylgeranyl diphosphate synthase

Purification

Purification on EC 2.5.1.29 - geranylgeranyl diphosphate synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, DEAE Sephadex a-50, hydroxylapatite
-
ammonium sulfate, DEAE-Sephadex, hydroxylapatite, Sephadex G-100, enzyme fraction may be a mixture of EC 2.5.1.1 and EC 2.5.1.29 or a single enzyme with two independent catalytic sites, see EC 2.5.1.1
-
ammonium sulfate, farnesylmethyl affinity gel
-
ammonium sulfate, phenyl Sepharose 6, Mono Q, Tosohaas-G3000, Mono P
-
anion exchange chromatography, aminophenylethyl diphosphate affinity column
-
DEAE-Sephadex
-
Mono Q, isoelectric focusing, Superose 12, partial purification
-
partial
poly(ethylene glycol) 6000, DEAE-Sephacel, aminophenethyl diphosphate affinity column
-
polyethylene glycol, DE-52 cellulose, DEAE Trisacryl-M, Reactive blue 4-agarose, Reactive red 120-agarose
-
purified by Co2+ affinity chromatography
recombinant enzyme
recombinant enzyme, urea, DEAE-52
-
recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3+) pLys RIL by glutathione affinity chromatography to homogeneity
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by metal affinity chromatography
recombinant his-tagged enzyme, Ni2+-column
recombinant His-tagged wild-type and mutant enzymes by nickel affinity chromatography from Escherichia coli strain JM109, removal of the tag by cleavage with Factor Xa
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration to homogeneity
-
recombinant N-terminally His-tagged enzyme from Escherichia coli, the His-tag is cleaved of by TEV
-
the histidine-tagged deletion mutants (from the carboxyl terminus) (DELTA-4, DELTA-8, DELTA-12 and DELTa-16) are overexpressed in Escherichia coli BL21 (DE3) and purified in a stable form by nickel affinity chromatography except for one mutant DELTA-16
-