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29 - 33.7
2-deoxy-D-glucose
0.00091 - 483.3
D-glucose
additional information
additional information
-
29
2-deoxy-D-glucose
-
wild-type enzyme
33.7
2-deoxy-D-glucose
wild-type, pH 9.0, 36°C
0.61
ATP
-
mutant L146R, 37°C, pH not specified in the puclication
0.7
ATP
-
wild-type, 37°C, pH not specified in the puclication. Kinetic constants at high and low concentrations of the fixed substrate: 0.5 mM D-glucose
2 - 3.7
ATP
-
37°C, pH 7.4, mutant enzyme A456V
4.92
ATP
-
mutant enzyme V62E
6.42
ATP
-
mutant enzyme V62K
9.5
ATP
pH 7.5, temperature not specified in the publication
11
ATP
-
37°C, pH 7.4, mutant enzyme K414E
14.9
ATP
-
mutant enzyme V62Q
25.7
ATP
-
mutant enzyme V62T
33.7
ATP
-
mutant enzyme V62F
43.1
ATP
-
mutant enzyme V62A
53.2
ATP
-
37°C, pH 7.4, wild-type enzyme
53.6
ATP
-
mutant enzyme V62M
54
ATP
-
wild type enzyme, at 25°C
59.9
ATP
-
mutant enzyme V62L
62.3
ATP
-
wild-type enzyme
68.4
ATP
-
wild-type, 37°C, pH not specified in the puclication
68.4
ATP
-
wild-type, 37°C, pH not specified in the puclication. Kinetic constants at high and low concentrations of the fixed substrate: 60 mM D-glucose
84.9
ATP
-
37°C, pH 7.4, mutant enzyme A456V
5.9
beta-D-glucose
-
co-substrate: ATP, mutant W99R/W167F in the presence of 20-30% glycerol and in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
6.1
beta-D-glucose
-
co-substrate: ATP, mutant W167F/W257F in the presence of 20-30% glycerol, pH and temperature not specified in the publication
6.37
beta-D-glucose
-
co-substrate: ATP, mutant W99R/W167F in the presence of 20-30% glycerol, pH and temperature not specified in the publication
8.47
beta-D-glucose
-
co-substrate: ATP, mutant W167F/W257F, pH and temperature not specified in the publication
9.43
beta-D-glucose
-
co-substrate: ATP, mutant W167F/W257F in the presence of 20-30% glycerol and in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
11.1
beta-D-glucose
-
co-substrate: ATP, mutant W99R/W167F in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
12.4
beta-D-glucose
-
co-substrate: ATP, mutant W99R/W167F, pH and temperature not specified in the publication
12.7
beta-D-glucose
-
co-substrate: ATP, mutant W167F/W257F in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
26.2
beta-D-glucose
-
co-substrate: ATP, mutant W99R/W257F in the presence of 20-30% glycerol, pH and temperature not specified in the publication
37.7
beta-D-glucose
-
co-substrate: ATP, wild-type in the presence of 20-30% glycerol, pH and temperature not specified in the publication
38.9
beta-D-glucose
-
co-substrate: ATP, mutant W99R/W257F in the presence of 20-30% glycerol and in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
60.5
beta-D-glucose
-
co-substrate: ATP, wild-type in the presence of 20-30% glycerol and in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
69.9
beta-D-glucose
-
co-substrate: ATP, mutant W99R/W257F, pH and temperature not specified in the publication
83.7
beta-D-glucose
-
co-substrate: ATP, wild-type, pH and temperature not specified in the publication
91.3
beta-D-glucose
-
co-substrate: ATP, mutant W99R/W257F in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
166
beta-D-glucose
-
co-substrate: ATP, wild-type in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
0.02
D-fructose
pH 7.5, temperature not specified in the publication
1.5
D-fructose
wild-type, pH 9.0, 36°C
3.69
D-fructose
30°C, pH 8.0
0.00091
D-glucose
-
mutant enzyme D205A, kcat less than 0.00091 s-1
0.007
D-glucose
-
mutant T228M, 37°C, pH not specified in the puclication
0.026
D-glucose
deletion mutant lacking residues 68-71, pH 7.6, 25 °C
0.037
D-glucose
deletion mutant lacking residues 66-71, pH 7.6, 25 °C
0.042
D-glucose
deletion mutant lacking residues 67-71, pH 7.6, 25 °C
0.18
D-glucose
-
truncated enzyme with removed helix alpha13
0.77
D-glucose
-
mutant L146R, 37°C, pH not specified in the puclication
1.06
D-glucose
-
pH 7.5, 22°C, soluble enzyme
1.67
D-glucose
-
pH 7.5, 22°C, immobilized enzyme
2 - 3.7
D-glucose
-
37°C, pH 7.4, mutant enzyme A456V
2.46
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant S336L
3.3
D-glucose
30°C, pH 8.0
3.47
D-glucose
at 37°C, pH 7
5.01
D-glucose
30°C, pH 8.0
10.8
D-glucose
mutant N216K, pH 9.0, 36°C
11
D-glucose
-
37°C, pH 7.4, mutant enzyme K414E
17.4
D-glucose
mutant enzyme W167F, in 25 mM HEPES (pH 7.4), at 37°C
17.7
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M197E
19.9
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant K414E
20.5
D-glucose
pH 7.5, temperature not specified in the publication
22.6
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant T65I
23.1
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant Y214A/V452A
24
D-glucose
mutant enzyme G72R, at 30°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol
29
D-glucose
-
wild type enzyme
29
D-glucose
deletion mutant lacking residues 70-71, pH 7.6, 25 °C
29.3
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant C252Y
29.8
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant G72R
33
D-glucose
deletion mutant lacking residue 71, pH 7.6, 25 °C
34
D-glucose
mutant enzyme G72R, at 37°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol
37
D-glucose
-
glucokinase with C-terminal 5 alanine addition
37.7
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M298K
38
D-glucose
-
wild-type enzyme
38
D-glucose
-
glucokinase with C-terminal 10 alanine addition
39
D-glucose
-
mutant enzyme Y214C
39.7
D-glucose
mutant D217H, pH 9.0, 36°C
40
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant K140E
40.1
D-glucose
wild-type, pH 7.6, 25 °C
42.1
D-glucose
mutant enzyme W257F, in 25 mM HEPES (pH 7.4), at 37°C
43.2
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant G68K
44.6
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant S263P
44.7
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant Y215A
46.8
D-glucose
mutant enzyme W99F, in 25 mM HEPES (pH 7.4), at 37°C
47.2
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V62M
48.3
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant P417R
50
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant C213R
50.2
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M197I/A379T
52.2
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant 454-Ala
52.6
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant E442K
53.2
D-glucose
-
37°C, pH 7.4, wild-type enzyme
54
D-glucose
mutant enzyme V62M, at 30°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol
56
D-glucose
mutant S64R/E67D/S69T, K0.5 value, Hill coefficient 1.7, pH 7.6, 25 °C
58.1
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M197I
60.4
D-glucose
wild type enzyme, in 25 mM HEPES (pH 7.4), at 37°C
60.6
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V91L
61
D-glucose
mutant enzyme V62M, at 37°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol
61.2
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant A379T
61.8
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V455M
62.6
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M197L
62.7
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant G68V
62.8
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, wild-type
64
D-glucose
-
wild type enzyme, at 25°C, in the presence of 0.02 mM activator N-[2-amino-4-fluoro-5-[(1-methyl-1H-imidazol-2-yl)sulfanyl]benzyl]-1,3-thiazol-2-amine
65.3
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant Y214C
65.6
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant A456V
67.1
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V389L
67.6
D-glucose
-
wild-type, 37°C, pH not specified in the puclication
74
D-glucose
wild type enzyme, at 30°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol
75
D-glucose
-
wild type enzyme, at 25°C, in the presence of 0.02 mM activator LY-2121260
83.3
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant S64P
84.9
D-glucose
-
37°C, pH 7.4, mutant enzyme A456V
93.39
D-glucose
pH and temperature not specified in the publication
101
D-glucose
wild type enzyme, at 37°C, in 100 mM Tris and 125 mM KCl, pH 7.4, in the presence of 14 mM beta-mercaptoethanol or 5 mM dithiothreitol
114
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant S64Y
117
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant Y214A
122
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V452L
195
D-glucose
-
recombinant HXK2, at 30°C, pH 8
219
D-glucose
wild-type, pH 9.0, 36°C
284
D-glucose
-
recombinant HXK1, at 30°C, pH 8
483.3
D-glucose
recombinant TbHK1
0.025
D-mannose
wild-type, pH 9.0, 36°C
3.78
D-mannose
30°C, pH 8.0
12.1
D-mannose
pH 7.5, temperature not specified in the publication
additional information
additional information
-
-
-
additional information
additional information
-
-
additional information
additional information
-
-
-
additional information
additional information
6000/min, wild-type and nonaggregating interface mutant hexokinase I
-
additional information
additional information
-
6000/min, wild-type and nonaggregating interface mutant hexokinase I
-
additional information
additional information
natural hexokinase: 68160/min, recombinant hexokinase expressed in Escherichia coli XL-1 Blue: 18913/min, at pH 7.5
-
additional information
additional information
-
natural hexokinase: 68160/min, recombinant hexokinase expressed in Escherichia coli XL-1 Blue: 18913/min, at pH 7.5
-
additional information
additional information
-
as the temperature is raised from 30°C to 47.5°C kcat decreases, falling to about 10 per sec (by 80%) at 50°C
-
additional information
additional information
-
osmolytes decrease kcat/KM in the order ofedecreasing efficiency NaCl, urea, trimethylamine N-oxide/glycerol, betains. For the organic osmolytes this order correlates with the degree of exclusion from protein/water interfaces
-
additional information
additional information
kinetic parameters of hexokinase activity in wine yeast strains
-
additional information
additional information
-
kinetic parameters of hexokinase activity in wine yeast strains
-
additional information
additional information
-
the osmolytes decreases kcat/KM in the order: NaCl/urea/trimethylamine-N-oxide dehydrate/glycerol/betaine. For the organic osmolytes this order correlates with the degree of exclusion from protein-water interfaces. Thus, the stronger the exclusion the weaker the perturbing effects on kcat/KM
-