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2.7.3.2: creatine kinase

This is an abbreviated version!
For detailed information about creatine kinase, go to the full flat file.

Word Map on EC 2.7.3.2

Reaction

ATP
+
Creatine
=
ADP
 +
phosphocreatine

Synonyms

adenosine triphosphate-creatine transphosphorylase, adenosine-5'-triphosphate: creatine phosphotransferase, ATP-creatine transphosphorylase, ATP: creatine N-phosphotransferase, ATP:creatine phosphotransferase, B-type creatine kinase, BB-CK, BB-type creatine kinase, BCK, brain creatine kinase, brain type creatine kinase, brain-type CK, brain-type creatine kinase, CK, CK MM, CK-B, CK-BB, CK-MB, CK-MM, ckb, CKM, CKMB, CKMBI, CKMiMi, creatine kinase, creatine kinase B, creatine kinase M-type, creatine kinase MB, creatine kinase muscle type, creatine kinase-MB, creatine N-phosphotransferase, creatine phosphokinase, creatine phosphotransferase, creatinine kinase, creatinine kinase MB, hBBCK, hMMCK, kinase, creatine (phosphorylating), M-CK, M1-CK, MB-CK, MCK, Mi-CK, MiMi-CK, mit-CK, mitochondrial creatine kinase, MM-CK, MM-type creatine kinase, More, MtCK, muscle creatine kinase, muscle type creatine kinase, muscle-type creatine kinase, phosphocreatine kinase, plasma creatine kinase, PSCKM, recombinant human brain-type creatine kinase, rHBCK, RM-CK, s-type CK, sarcomeric CK, sMiCK, sMtCK, u-type CK, ubiquitous CK, ubiquitous MtCK, uMiCK, uMtCK, zMMCK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.3 Phosphotransferases with a nitrogenous group as acceptor
                2.7.3.2 creatine kinase

Cloned

Cloned on EC 2.7.3.2 - creatine kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
both subunits are co-expressed in Escherichia coli BL21(DE3) cells
brain isoform
-
DNA and amino acid sequence determination and analysis, optimization of overexpression of full length enzyme and mature enzyme lacking the mitochondrial targeting sequence in Escherichia coli
-
expressed in Escherichia coli
expressed in Escherichia coli as a fusion protein with maltose-binding protein, mitochondrial isoform sMiCK
-
expressed in Escherichia coli as a fusion protein with maltose-binding protein, muscle-type isoform MCK
-
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Rosetta 2 (DE3) cells
expression in Escherichia coli, mostly in insoluble form, after optimization of the conditions the enzyme does not aggregate and shows multi-kinetic phases
-
expression of soluble monomeric or dimeric mutants
-
expression of soluble wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene Ckb, brain cDNA library screening for enzyme interaction partners
gene Ckb, real-time quantitative reverse-transcription enzyme expression analysis. Compared to Neuro-2a cells expressing a control construct, expression of mHTT (109Q) significantly inhibits CKB promoter activity
overexpressed as a soluble form in Escherichia coli
-
overexpression of soluble wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), triple mutant enzyme R147A/R151A/D209A is expressed as insoluble, aggregated protein
recombinant expression of Fc-III-tagged enzyme and of His-tagged enzyme in Escherichia coli strain BL21 (DE3)-plysS, respectively, the Fc-III-tagged GFP-fusion muscle creatine kinase is used as a model system to investigate effects of the Fc-III tag on activities and stabilities of recombinantly expressed multicysteine-containing proteins