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2.7.3.2: creatine kinase

This is an abbreviated version!
For detailed information about creatine kinase, go to the full flat file.

Word Map on EC 2.7.3.2

Reaction

ATP
+
Creatine
=
ADP
 +
phosphocreatine

Synonyms

adenosine triphosphate-creatine transphosphorylase, adenosine-5'-triphosphate: creatine phosphotransferase, ATP-creatine transphosphorylase, ATP: creatine N-phosphotransferase, ATP:creatine phosphotransferase, B-type creatine kinase, BB-CK, BB-type creatine kinase, BCK, brain creatine kinase, brain type creatine kinase, brain-type CK, brain-type creatine kinase, CK, CK MM, CK-B, CK-BB, CK-MB, CK-MM, ckb, CKM, CKMB, CKMBI, CKMiMi, creatine kinase, creatine kinase B, creatine kinase M-type, creatine kinase MB, creatine kinase muscle type, creatine kinase-MB, creatine N-phosphotransferase, creatine phosphokinase, creatine phosphotransferase, creatinine kinase, creatinine kinase MB, hBBCK, hMMCK, kinase, creatine (phosphorylating), M-CK, M1-CK, MB-CK, MCK, Mi-CK, MiMi-CK, mit-CK, mitochondrial creatine kinase, MM-CK, MM-type creatine kinase, More, MtCK, muscle creatine kinase, muscle type creatine kinase, muscle-type creatine kinase, phosphocreatine kinase, plasma creatine kinase, PSCKM, recombinant human brain-type creatine kinase, rHBCK, RM-CK, s-type CK, sarcomeric CK, sMiCK, sMtCK, u-type CK, ubiquitous CK, ubiquitous MtCK, uMiCK, uMtCK, zMMCK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.3 Phosphotransferases with a nitrogenous group as acceptor
                2.7.3.2 creatine kinase

Purification

Purification on EC 2.7.3.2 - creatine kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 interconvertible forms of enzyme formed by reversible oxidation of sulfhydryl groups
-
5 varieties of isozyme MM-CK
-
affinity chromatography
cellulose DE-52 column chromatography and Sephacryl S-200 gel filtration
cytosolic and mitochondrial isozymes from skeletal muscle by affinity chromatography
-
DEAE Sepharose column chromatography and Sephadex G-200 gel filtration
-
isolation of the octameric enzyme form from eyes by gel filtration, and affinity and anion exchange chromatgraphy
isozyme CK-II, CK-III, CK-IV
-
isozyme Mia-CK
-
isozyme MM-CK
native enzyme from muscle by anion exchange chromatography and gel filtration
-
native isozyme BCK in synaptic vesicles and membranes of neuronal synaptosomes and mitochondria
Ni-affinity column chromatography
Ni-Hitrap column chromatography
Ni-NTA column chromatography
Ni-NTA column chromatography and His GraviTrap column chromatography
Ni-NTA column chromatography, and Superdex 200 gel filtration
Ni-NTA resin affinity column chromatography, His GraviTrap column chromatography, and streptavidin magnetic resin chromatography
overview
partially preparation of mitochondria
preparation of catalytically active hybrids of brain and muscle enzymes
recombinant enzyme, inclusion bodies
-
recombinant Fc-III-tagged enzyme from Escherichia coli strain BL21 (DE3)-plysS by IgG-Fc immunoaffinity chromatography, recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3)-plysS by nickel affinity chromatography
recombinant full length enzyme and mature enzyme from Escherichia colito near homogeneity by affinity and cation exchange chromatography
-
recombinant soluble monomeric or dimeric mutant enzymes by affinity chromatography, native enzyme from skeletal muscle, at high ionic strength, low temperature, and by fractionation with ethanol, or by affinity and ion exchange chromatography
-
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)
typical ubiquitous mitochondrial enzyme from heart muscle tissue, atypical ubiquitous mitochondrial from serum
-