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2.7.7.1: nicotinamide-nucleotide adenylyltransferase

This is an abbreviated version!
For detailed information about nicotinamide-nucleotide adenylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.1

Reaction

ATP
+
nicotinamide ribonucleotide
=
diphosphate
 +
NAD+

Synonyms

adenosine triphosphate-nicotinamide mononucleotide transadenylase, adenylyltransferase, nicotinamide mononucleotide, Arabidopsis thaliana nicotinate/nicotinamide mononucleotide adenyltransferase, AtNMNAT, ATP:NMN adenylyltransferase, diphosphopyridine nucleotide pyrophosphorylase, Drosophila NMNAT, GlNMNAT, hNMNAT, hNMNAT-1, hNMNAT-2, human nicotinamide mononucleotide adenylyl-transferase, m-nonN-Nmnat1, mononucleotide adenylyltransferase, More, NAD synthase, NAD+ pyrophosphorylase, nadD, NadM-Nudix, NadR, NaMNAT, nicotinamide 5'-mononucleotide adenylyltransferase, nicotinamide 5'-mononucleotide adenylyltransferase-2, nicotinamide 5-mononucleotide adenylyltransferase-2, nicotinamide adenine dinucleotide pyrophosphorylase, nicotinamide mononucleotide adenylyl-transferase, nicotinamide mononucleotide adenylyltransferase, nicotinamide mononucleotide adenylyltransferase 1, nicotinamide mononucleotide adenylyltransferase 2, nicotinamide mononucleotide adenylyltransferase type 1, nicotinamide mononucleotide adenylyltransferase1, nicotinamide mononucleotide adenylyltransferase2, nicotinamide/nicotinate mononucleotide adenylyltransferase, nicotinamide/nicotinic acid mononucleotide adenylyltransferase, nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1, nicotinate/nicotinamide mononucleotide adenyltransferase, NMN adenylyl transferase 1, NMN adenylyltransferase, NMN adenylyltransferase/ADP-ribose pyrophosphatase, NMN AT, NMN-adenylyltransferase, NMN/NaMN adenylyltransferase, NMN/NaMN adenylyltransferase 2, NMN/NaMN adenylyltransferase 3, NMNAT, NMNAT-2, NMNAT1, NMNAT2, NMNAT3, NMNATase, non-nuclear-localized-Nmnat1, PF3D7_1327600, PNAT, Pof1, pyridine nucleotide adenylyltransferase, Tc00.1047053507047.170, TK0067, TTHA1780, yNMNAT-1, yNMNAT-2

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.1 nicotinamide-nucleotide adenylyltransferase

Purification

Purification on EC 2.7.7.1 - nicotinamide-nucleotide adenylyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
co-purification of recombinant His6-tagged NMNAT1 and GST-tagged NML from Escherichia coli, His6-NMNAT1 is pulled down by beads loaded with GST-NML, but not by GST suggesting that the two proteins interact directly
erythrocytes, partial
-
FPLC reveals microheterogeneity, possibly through poly-ADP-ribosylation of the enzyme
-
Ni-NTA affinity chromatography
Ni-NTA agarose bead chromatography
partial
prepared from cerebrum for activity assays
recombinant enzyme
-
recombinant enzyme NMNAT-2
-
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography
recombinant His-tagged isozymes NMNAT1, NMNAT2, and NMNAT3 from Escherichia coli strain BL21(DE3) by nickel or TALON affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 Gold (DE3) by anion exchange and nickel affinity chromatography, tag cleavage by thrombin, dialysis, and ultrafiltration
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Talon affinity column chromatography