1.1.2.3: L-lactate dehydrogenase (cytochrome)

This is an abbreviated version!
For detailed information about L-lactate dehydrogenase (cytochrome), go to the full flat file.

Word Map on EC 1.1.2.3

Reaction

(S)-lactate
+ 2 ferricytochrome c =
pyruvate
+ 2 ferrocytochrome c + 2 H+

Synonyms

Cyb2, cytochrome b2, cytochrome c oxido reductase, FC b2, Fcb2, flavocytochrome b, flavocytochrome b2, iLDH, L(+)-lactate:cytochrome c oxidoreductase, L-(+)-lactate ferricytochrome c oxidoreductase, L-lactate cytochrome c oxidoreductase, L-lactate cytochrome c reductase, L-lactate dehydrogenase [Cytochrome], L-lactate ferricytochrome C oxidoreductase, L-lactate-cytochrome c oxidoreductase, L-lactate-cytochrome c-oxidoreductase, L-lactate: cytochrome c oxidoreductase, L-lactate:cytochrome c oxidoreductase, L-lactate:cytochrome c-oxidoreductase, L-LCR, L-LDH (FMN-dependent), lactate dehydrogenase (cytochrome), lactic acid dehydrogenase, lactic cytochrome c reductase, NAD+ - independent LDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.2 With a cytochrome as acceptor
                1.1.2.3 L-lactate dehydrogenase (cytochrome)

Crystallization

Crystallization on EC 1.1.2.3 - L-lactate dehydrogenase (cytochrome)

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CRYSTALLIZATION/commentary
ORGANISM
UNIPROT
LITERATURE
crystal structure of flavocytochrome b2 solved at 3.0 A resolution by the method of multiple isomorphous replacement with anomalous scattering
-
crystal structure of mutant H723Q bound with pyruvate is determined at 2.8 A
-
crystallization of flavin binding domain and intact enzyme, hanging drop vapor diffusion method
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crystallization of Y143F mutant, vapor diffusion technique in presence of PEG 4000
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Fcb2 free and in complex with sulfite, X-ray diffraction structure analysis at 2.3-2.6 A resolution
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hanging drop vapor diffusion method, using 20% polyethylene glycol 4000, 0.1 M MES, pH 6.5, and 0.2 M MgCl2, at 18°C
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mutant L230A in complex with phenylglyoxalate, mutant A198G/L230A in complex with sulfite, mutant A198G/L230A in complex with pyruvate
-
used as essential purification step, crystallized as DNA complex
-