1.14.12.13: 2-halobenzoate 1,2-dioxygenase

This is an abbreviated version!
For detailed information about 2-halobenzoate 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.14.12.13

Reaction

a 2-halobenzoate +

NADH
+
H+
+
O2
=
catechol
+ a halide anion +
NAD+
+
CO2

Synonyms

(chloro)benzoate dioxygenase, 2-chlorobenzoate 1,2-dioxygenase, 2-halobenzoate 1,2-dioxygenase, 2-halobenzoate dioxygenase, 2-halobenzoate dioxygenase system, BenA, CbdAB, cbdABC

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
                1.14.12.13 2-halobenzoate 1,2-dioxygenase

Specific Activity

Specific Activity on EC 1.14.12.13 - 2-halobenzoate 1,2-dioxygenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0013
-
cells are grown on 3-chlorobenzoate, benzoate as substrate for assay
0.0024
-
recombinant cells are grown on LB/IPTG, benzoate as substrate for assay
0.0032
-
cells are grown on benzoate, benzoate as substrate for assay
0.006
-
with 4-chlorobenzoate as substrate
0.0085
-
crude extract, 3.4 mg protein per ml in the assay
0.018 - 0.023
-
cells grown on 2-clorobenzoate or 2,5-dichlorobenzoate, enzyme activity for the conversion of both substrates
0.032
-
with 2-iodobenzoate as substrate
0.043
-
after anion exchange: fraction A + B + NADH + Fe2+
0.073
-
with 3-chlorobenzoate as substrate
0.195
-
with benzoate as substrate
0.303
-
with 2-bromobenzoate as substrate
0.458
-
with 2-chlorobenzoate as substrate
0.53
-
activity of component A after gel filtration in presence of component B
1.57
-
30°C, pH 6.5
4
-
activity of component B in presence of component A
additional information
-
activity is similarly influenced by pH, temperature, concentration of oxygen, protein, Fe2+, FAD and NADH in assay medium independent if the cells are grown on 2-chlorobenzoate or 2,5-dichlorobenzoate