1.14.13.2: 4-hydroxybenzoate 3-monooxygenase

This is an abbreviated version!
For detailed information about 4-hydroxybenzoate 3-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.2

Reaction

4-hydroxybenzoate
+
NADPH
+
H+
+
O2
=
3,4-dihydroxybenzoate
+
NADP+
+
H2O

Synonyms

4-HBA 3-hydroxylase, 4-HBA 3-monooxygenase, 4-HBA-3-hydroxylase, 4-HBMO, 4-hydroxybenzoate 3-hydroxylase, 4-hydroxybenzoate 3-monooxygenase, 4-hydroxybenzoate hydroxylase, 4-hydroxybenzoate monooxygenase, 4-hydroxybenzoic hydroxylase, 4HBA 3-hydroxylase, BxeA2040, HBH, m-hydroxybenzoate hydroxylase, MobA, ncgl1032, oxygenase, 4-hydroxybenzoate 3-mono-, p-hydroxybenzoate hydroxylase, p-hydroxybenzoate-3-hydroxylase, p-hydroxybenzoic acid hydrolase, p-hydroxybenzoic acid hydroxylase, p-hydroxybenzoic hydroxylase, para-hydroxybenzoate hydroxylase, PHBAD, PHBH, PHBHase, PobA, pobACg, POHBase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.2 4-hydroxybenzoate 3-monooxygenase

Storage Stability

Storage Stability on EC 1.14.13.2 - 4-hydroxybenzoate 3-monooxygenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, 5 mM potassium phosphate, pH 7.5, 5% glycerol, stable for several months
-
0°C-4°C, as a precipitate under a solution of 50 mM potassium phosphate and 0.5 mM EDTA, pH 6.5-7.0, with 70% saturated ammonium sulfate, idefinitely stable
-
4°C, 24 h, 40% loss of activity without stabilizer, 10% loss of activity in presence of 4-hydroxybenzoate, FAD and EDTA
-
4°C, 50 mM phosphate buffer, 50 mM Tris-HCl, pH 8.0, 10% glycerol, 10 mM EDTA, loss of activity after 72 h
-
4°C, as ammonium sulfate paste, indefinitely stable
-