18.104.22.168: 2,3-bis-O-geranylgeranyl-sn-glycero-phospholipid reductase
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For detailed information about 2,3-bis-O-geranylgeranyl-sn-glycero-phospholipid reductase, go to the full flat file.
+ 16 oxidized ferredoxin [iron-sulfur] cluster = + 16 reduced ferredoxin [iron-sulfur] cluster + 16 H+
AF0464, CrtI homologue, digeranylgeranylglycerophospholipid reductase, EC 22.214.171.124, geranylgeranyl reductase, GGR, MA1484, MA1492, MA_1484, MA_1492, phytoene dehydrogenase family protein, Sa-GGR, SaGGR, Ta0516
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Crystallization on EC 126.96.36.199 - 2,3-bis-O-geranylgeranyl-sn-glycero-phospholipid reductase
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crystals are obtained using 3.5-4.5 M sodium formate with 0.1 M sodium acetate buffer, pH 4.6, as the precipitant solution and 1.0 mM geranylgeranyl diphosphate is added for co-crystallization, 1.8 A resolution
wild-type and mutant enzymes, in apoform or complexed with geranygeranyl diphosphate, sitting drop vapor diffusion, mixing of 10 mg/ml protein in 25 mM HEPES, pH 7.4, with well solution containing 0.1M Tris, pH 7.5, 10% PEG 3350, and 0.2 M L-proline, in a 1:1 ratio, sparse matrix-screening crystallization method for method screening, addition of 5 mM GGPP for ligand-bound crystals, 2 days, X-ray diffractin structure determination and analysis, modeling
in complex with flavin adenine dinucleotide, to 1.6 A resolution. Substrate binding likely involves conformational changes, which are coupled to the two conformational states of the FAD cofactor