1.4.1.13: glutamate synthase (NADPH)

This is an abbreviated version!
For detailed information about glutamate synthase (NADPH), go to the full flat file.

Word Map on EC 1.4.1.13

Reaction

2 L-glutamate +

NADP+
=
L-glutamine
+
2-oxoglutarate
+
NADPH
+
H+

Synonyms

EC 2.6.1.53, EhNO1, EhNO2, gltA, gltB, GltB1, GltB2, gltD, GltS, glutamate synthetase (NADP), glutamine amide-2-oxoglutarate aminotransferase (oxidoreductase, NADP), glutamine-ketoglutaric aminotransferase, GOGAT, L-glutamate synthase, L-glutamate synthetase, L-glutamine:2-oxoglutarate aminotransferase, NADPH oxidizing, NADPH-dependent glutamate synthase, NADPH-GltS, NADPH-glutamate synthase, NADPH-GOGAT, NADPH-linked glutamate synthase, pGLTY1, pGLTY2, pGLTZ, PH0876, PH1873, synthase, glutamate (reduced nicotinamide adenine dinucleotide phosphate)

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.13 glutamate synthase (NADPH)

Engineering

Engineering on EC 1.4.1.13 - glutamate synthase (NADPH)

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C47A
-
beta subunit, no significant effect on NADPH:iodonitrotetrazolium oxidoreductase activity of subunit, complete loss of activity of enzyme
C50A
-
beta subunit, no significant effect on NADPH:iodonitrotetrazolium oxidoreductase activity of subunit, complete loss of activity of enzyme
C55A
-
beta subunit, no significant effect on NADPH:iodonitrotetrazolium oxidoreductase activity of subunit, complete loss of activity of enzyme
C59A
-
beta subunit, no significant effect on NADPH:iodonitrotetrazolium oxidoreductase activity of subunit, complete loss of activity of enzyme
G298A
-
mutant with an approximately 10fold decrease of the affinity of the enzyme for pyridine nucleotides with little or no effect on the rate of the enzyme reduction by NADPH, maintains the ability to bind NADPH and FAD, is catalytically active in the NADPH:iodonitrotetrazolium oxidoreductase reaction and has a monomeric state, mutation leads to production of insoluble protein under conditions that yield large amounts of soluble wild-type enzyme and to production of smaller amounts of enzyme