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1.5.1.3: dihydrofolate reductase

This is an abbreviated version!
For detailed information about dihydrofolate reductase, go to the full flat file.

Word Map on EC 1.5.1.3

Reaction

5,6,7,8-tetrahydrofolate
+
NADP+
=
7,8-dihydrofolate
 +
NADPH
 +
H+

Synonyms

5,6,7,8-tetrahydrofolate: NADP+ oxidoreductase, 7,8-dihydrofolate reductase, At2g16370, At4g34570, bifunctional dihydrofolate reductase-thymidylate synthase, bifunctional TS-DHFR, BmDHFR, dehydrogenase, tetrahydrofolate, DFR-TS, DFR1, DfrA, DfrB, DHFR, DHFR type IIIC, DHFR-TS, DHFR-TS1, DHFR-TS2, DHFR2, DHFRL1, DHFRLS, dihydrofolate reductase, dihydrofolate reductase-like, dihydrofolate reductase-thymidylate synthase, dihydrofolate reductase:thymidylate synthase, dihydrofolic acid reductase, dihydrofolic reductase, EC 1.5.1.4, ecDHFR, folA, folA3, folic acid reductase, folic reductase, FolM, hDHFR, hDHFR-1, hDHFR-2, HjDHFR, hvDHFR1, hvDHFR2, LAU_0427, LBRM_06_0830, mDHFR, mjDHFR, More, myDHFR, NADPH-dihydrofolate reductase, pcDHFR, PKNH_0509600, ppDHFR, pteridine reductase, pteridine reductase:dihydrofolate reductase, PTR2, R-plasmid-encoded dihydrofolate reductase, R67 DHFR, R67 dihydrofolate reductase, reductase, dihydrofolate, S3DHFR, Smdhfr, Smp 175230, spDHFR, svDHFR, tcptr1, tetrahydrofolate dehydrogenase, THY-1, THY-2, thymidylate synthase-dihydrofolate reductase, thymidylate synthetase-dihydrofolate reductase, Trimethoprim resistance protein, TS-DHFR, WUBG_00817

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.3 dihydrofolate reductase

Temperature Stability

Temperature Stability on EC 1.5.1.3 - dihydrofolate reductase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0
cold denaturation below
30 - 60
when temperature is higher than 30°C, the enzyme activity is decreased rapidly but the comparatively lower velocity is maintained till 60°C
37.5
-
the melting temperature of the apo-enzyme is 37.5°C and is not affected by pH over the range 5.0-9.0
4
-
above, for extended length of time, unstable
40 - 50
-
the enzyme is able to perform its full catalytic rate for at least 1 h at temperatures up to 40°C. At higher temperatures the steady-state rate decreases rapidly with a half-life of inactivation of around 13 min at 45°C and 7 min at 50°C
40.9
melting temperature, mutant linear ANLYF-G7
42
melting temperature, mutant M1A/M16N/M20L/M42Y/C85A/M92F/C152S
44.3
melting temperature, mutant linear ANLYF-G8
44.5
melting temperature, mutant linear ANLYF-G6
47
-
half-life 5.18 min, in presence of GroEL (minichaperone) half-life 12.35 min, in presence of GroEL-GroES half-life 21.32 min
49.3
melting temperature, mutant circular ANLYF-G8
49.5
melting temperature, mutant circular ANLYF-G6
50.2
melting temperature, wild-type
51.3
melting temperature, mutant circular ANLYF-G7
53
-
wild-type: decay rate constant 0.12 per min, mutant enzymes 0.06-0.08 per min
64
-
rapid loss of activity in absence of substrate above 64°C
67
-
the melting temperature is 67°C
68
AB986558
lowering the pH from 8 to 6 induces significant structural formation to the enzyme
80
-
half-life: about 60 min, half-life with NADPH: more than 360 min, half-life with 7,8-dihydrofolate: more than 240 min, half-life with 1M urea: 26-28 min
83
-
midpoint of the thermal unfolding
additional information