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2.4.2.4: thymidine phosphorylase

This is an abbreviated version!
For detailed information about thymidine phosphorylase, go to the full flat file.

Word Map on EC 2.4.2.4

Reaction

thymidine
+
phosphate
=
thymine
 +
2-deoxy-alpha-D-ribose 1-phosphate

Synonyms

angiogenic factor platelet-derived endothelial cell growth factor/thymidine phosphorylase, animal growth regulators, blood platelet-derived endothelial cell growth factors, blood platelet-derived endothelial cell growth factor, deoA, deoxythymidine phosphorylase, dthdpase, EC 2.4.2.23, gliostatin, gliostatins, GLS, HTP, More, PD-ECGF, PD-ECGF/TP, PD-ECGF/TP platelet-derived endothelial cell growth factor, PDECGF, phosphorylase, thymidine, platelet derived endothelial cell growth factor, platelet derived endothelial cell grwoth factor, platelet derived-endothelial cell growth factor, platelet-derived endothelial cell growth factor, platelet-derived endothelial cell growth factor/thymidine phosphorylase, platelet-derived endotherial cell growth factor, ppnP, pynpase, pyrimidine deoxynucleoside phosphorylase, pyrimidine nucleoside phosphorylase, pyrimidine phosphorylase, pyrimidine/purine nucleoside phosphorylase, TDRPASE, ThdPase, thymidine phosphorylase, thymidine Pi deoxyribosyltransferase, thymidine-orthophosphate deoxyribosyltransferase, thymidine:orthophosphate deoxy-D-ribosyltransferase, Tpase, TYMP

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.4 thymidine phosphorylase

Crystallization

Crystallization on EC 2.4.2.4 - thymidine phosphorylase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
analysis of crystal structure of recombinant Escherichia coli TP in complex with 2'-fluoro-3'-azido-2',3'-dideoxyuridine (N3FddU) and 3'-azidothymidine (AZT) at 1.50 A and 1.52 A resolutions, respectively. Analysis of the free enzyme crystal structure at 2.8 A resolution
hanging drop vapor diffusion method
-
analysis of crystal structure of recombinant human TP in complex with 5-iodouracil (5IUR) at 3.0 A and 2.5 A resolutions, respectively
enzyme complexed with TPI
-
purified recombinant thymidine phosphorylase, free and in complex with 5-iodouracil, X-ray diffraction structure determination and analysis at 3.0 A and 2.5 A resolutions, respectively
structure of enzyme from crystals grown in the presence of thymidine
structure of HTP bound to the small molecule inhibitor 5-chloro-6-[1-(2-iminopyrrolidinyl)methyl]uracil hydrochloride, glutathione S-transferase fused to thymidine phosphorylase residues 12–482 (based on Swissprot accession number P19971) via a thrombin-cleavable linker, crystallizationd at 15°C using the hanging-drop vapor method
X-ray diffraction structure determination and analysis of the free enzyme at 3.5 A resolution, and of the in complex with the small and potent inhibitor 5-chloro-6-[1-(2-iminopyrrolidinyl)methyl] uracil at 2.1 A resolution
-
sitting drop vapor diffusion method, using 0.2 M potassium thiocyanate, 0.1 M bis-Tris propane, 20% (w/v) PEG 3350 pH 7.5 (unliganded enzyme), or 0.1 M Bicine, 1.6 M (NH4)2SO4 pH 9.0 (in complex with thymidine), or 0.1 M citric acid, 10% (w/v) PEG 6000 pH 5.0 (in complex with uridine)