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2.5.1.34: 4-dimethylallyltryptophan synthase

This is an abbreviated version!
For detailed information about 4-dimethylallyltryptophan synthase, go to the full flat file.

Word Map on EC 2.5.1.34

Reaction

dimethylallyl diphosphate
+
L-tryptophan
=
diphosphate
 +
4-(3-methylbut-2-enyl)-L-tryptophan

Synonyms

4-(gamma,gamma-dimethylallyl)tryptophan synthase, 4-dimethylallyltryptophan synthase, 4-DMATS, 7-dimethylallyltryptophan synthase, 7-DMATS, C4-prenyltransferase, dimethylallyl-tryptophan synthase, dimethylallylpyrophosphate:L-tryptophan dimethylallyltransferase, dimethylallylpyrophosphate:tryptophan dimethylallyl transferase, dimethylallylpyrophosphate:tryptophan dimethylallyltransferase, dimethylallyltransferase, tryptophan, dimethylallyltryptophan synthase, dimethylallyltryptophan synthetase, DMAT synthase, DMAT synthetase, DMATS, DmaW, DmaW2, FgaPT2, IfgA, MaPT, Proq05g069320, tryptophan C4-prenyltransferase, tryptophan C4-prenyltransferase FgaPT2, tryptophan C4-PT

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.34 4-dimethylallyltryptophan synthase

Cloned

Cloned on EC 2.5.1.34 - 4-dimethylallyltryptophan synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as a His-tagged fusion protein
-
expressed as a recombinant protein
-
expressed in Escherichia coli BL21(DE3) pLysS cells
-
expression in Escherichia coli
expression in Saccharomyces cerevisiae
-
expression of His-tagged 4-DMATS in Escherichia coli strain BL21 Star (DE3)
expression of His-tagged 4-DMATS in Escherichia coli strains XL1 Blue or M15 or in Saccharomyces cerevisiae strain INVSc1
gene 4-DMATS, sequence comparison
gene 4-DMTAS, recombinant overexpression in Escherichia coli strain BL21 (DE3)pLysS
gene fgaPT2, DNA and amino acid sequence determination and analysis, overexpression of His6-tagged enzyme in Saccharomyces cerevisiae INVSc1
into pGEM-T and subsequently into pQE60 for expression in Escherichia coli XL1 Blue MRF cells
overexpressed in Escherichia coli as a C-terminal His-tagged fusion protein
-
recombinant expression
-
recombinant expression of the soluble enzyme in Escherichia coli strain BL21 Star™ (DE3)