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2.6.1.44: alanine-glyoxylate transaminase

This is an abbreviated version!
For detailed information about alanine-glyoxylate transaminase, go to the full flat file.

Word Map on EC 2.6.1.44

Reaction

L-alanine
+
glyoxylate
=
pyruvate
 +
glycine

Synonyms

3-hydroxykynurenine transaminase, 3-hydroxykynurenine transaminase/alanine glyoxylate transaminase, AGT, AGT-Ma, AGT-Mi, AGT1, AGT2, AGX, AGXT, AGXT2, alanine aminotransferase, alanine glyoxalate transaminase 2, alanine glyoxylate aminotransferase, alanine-glyoxalate aminotransferase, alanine-glyoxalate transaminase 1, alanine-glyoxylate aminotransferase, alanine-glyoxylate aminotransferase 2, alanine-glyoxylate aminotransferase isoenzyme 2, alanine-glyoxylate aminotransferase-2, alanine-glyoxylic aminotransferase, alanine: glyoxylate aminotransferase, alanine:2-oxoglutarate aminotransferase, alanine:glyoxylate aminotransferase, alanine:glyoxylate aminotransferase 1, alanine:glyoxylate aminotransferase 2, alanine:glyoxylate aminotransferase type 1, alanine:glyoxylate aminotransferase/beta-alanine:pyruvate aminotransferase, aminotransferase 3, AT3, At3g08860, cytosolic alanine aminotransferase, D-AIB AT, EC 2.6.1.51, GGT, glyoxylate aminotransferase AGT1, L-alanine-glycine transaminase, L-alanine-glyoxylate aminotransferase, L-alanine:glyoxylate aminotransferase, More, serine pyruvate aminotransferase, serine:pyruvate/alanine:glyoxylate aminotransferase, SPT/AGT

ECTree

     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.44 alanine-glyoxylate transaminase

Cloned

Cloned on EC 2.6.1.44 - alanine-glyoxylate transaminase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AGXT cDNA cloned, AGXT*LTM expressed as a GST-fusion protein in Escherichia coli BL21(RIL) and in Sf9 cells
-
cDNA for AGT1 expressed in Escherichia coli BL-21
cDNA isolation
-
cloned and expressed in Escherichia coli JM109
-
Escherichia coli DH1 transformed with pRspt10
-
expressed in Ashbya gossypii strain ATCC10895
-
expressed in COS-1a cells
-
expressed in COS-7 cells and human umbilical vein endothelial cells with a C-terminal FLAG epitope tag
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21-CodonPlus-RIPL mutants auxotrophic for the amino acids L-alanine and beta-alanine
expressed in Escherichia coli JM109 cells
expressed in Mus musculus embryonic stem cells
-
expressed in Sf9 cells
expressed in stably transformed CHO cells
-
expression in CHO cell
-
expression in Escherichia coli
expression in HeLa cell
-
expression of of untagged alanine-glyoxylate aminotransferase in Escherichia coli
-
expression vectors of the enhanced green fluorescent protein-tagged alanine:glyoxylate aminotransferase and deletion mutants are introduced into HeLa cells to identify the peroxisomal targeting signal of the alanine:glyoxylate aminotransferase
-
for sequence determination, GFP-fusion proteins used in localization experiments
gene AGT, recombinant expression of wild-type and mutant G41R enzymes in Escherichia coli strain BL21, recombinant expression of wild-type AGT and the G41R variant in CHO-GO cells, that overexpress glycolate oxidase
gene AGXT, quantitative real-time reverse transcription-PCR enzyme expression analysis
gene AGXT, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli
gene AGXT, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21
gene AGXT2, real-time RT-PCR enzyme expression analysis
His-tagged protein expressed in Escherichia coli JM109, expressed in CHO cells
-
human AGT can substitute for function of yeast Agx1 (yeast alanine:glyoxylate aminotransferase) and that mutations associated with disease in humans show reduced growth in yeast. The reduced growth of minor allele mutants reflects reduced protein levels, indicating that these proteins are less stable than wild-type AGT in yeast
-
human AGT expressed in Escherichia coli B834(DE3)
-
locus At3g08860, DNA and amino acid sequence determination, analysis, and comparisons, recombinant expression of the enzyme lacking the N-terminal signal sequence and functional complementation of two Escherichia coli mutants (panD and HYE032) auxotrophic for the amino acids, L-alanine (proteinogenic) and beta-alanine (non-proteinogenic). The enzyme is not able to complement the Escherichia coli mutant DL39, that harbors a mutation in the tyrosine aminotransferase (tyrB) gene which leads to auxotrophy for L-tyrosine and L-phenylalanine. Recombinant expression of His-tagged enzyme in Escherichia coli
mammalian expression vector pHYK, expressed in COS-1 cells
-
mitochondrial and peroxisomal isozymes generated from a single gene by alternative transcription initiation, cloned and expressed in Escherichia coli DH5 and ER1458
-
sequencing of the 5' region of the AGT gene
single AGT gene cloned
-
used in complementation experiments, AGT1-eGFP fusion protein used in localization experiments
-
used in knockout experiments
wild-type and G82E mutant His-tagged enzyme expressed in Escherichia coli