2.7.1.78: polynucleotide 5'-hydroxyl-kinase

This is an abbreviated version!
For detailed information about polynucleotide 5'-hydroxyl-kinase, go to the full flat file.

Reaction

Synonyms

5' end-specific RNA/DNA kinase, 5' polynucleotide kinase, 5'-hydroxyl polynucleotide kinase, 5'-hydroxyl polyribonucleotide kinase, 5'-hydroxyl RNA kinase, 5'-OH polynucleotide kinase, ATP:5'-dephosphopolynucleotide 5'-phosphatase, cleavage and polyadenylation factor I subunit, CLP1, Clp1-like protein, DNA 5'-hydroxyl kinase, DNA kinase, Grc3, HD-Pnk, HEAB, hPNKP, kinase (phosphorylating), polynucleotide 5'-hydroxyl, More, mPNKP, Nol9, PALF, PhoClp1, PNK, Pnk1, PNKP, polynucleotide 5'-hydroxyl kinase (phosphorylating), polynucleotide 5'-hydroxyl-kinase, polynucleotide kinase, polynucleotide kinase and aprataxin-like forkhead-associated, polynucleotide kinase phosphatase, polynucleotide kinase-phosphatase, polynucleotide kinase/phosphatase, RNA-specific polynucleotide kinase, Rnl1, RPNK, T4 PNK, T4 polynucleotide kinase, T4 polynucleotide kinase/phosphatase, tRNA endonuclease complex, TSEN

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.1 Phosphotransferases with an alcohol group as acceptor

                2.7.1.78 polynucleotide 5'-hydroxyl-kinase

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Results	in table
32	Activating Compound
1445	AA Sequence
14	Application
1	CAS Registry Number
20	Cloned(Commentary)
104	Cofactor
8	Crystallization (Commentary)
162	Disease/ Diagnostics
30	General Information
10	General Stability
7	IC50 Value
148	Inhibitors
13	Ki Value [mM]
55	KM Value [mM]
9	Localization
114	Metals/Ions
29	Molecular Weight [Da]
84	Natural Substrates/ Products (Substrates)
103	Organism
51	PDB ID
44	pH Optimum
14	pH Range
1	pH Stability
3	pI Value
62	Protein Variants
37	Purification (Commentary)
9	Reaction
9	Reaction Type
99	Reference
39	Source Tissue
18	Specific Activity [micromol/min/mg]
8	Storage Stability
315	Substrates and Products (Substrate)
35	Subunits
130	Synonyms
1	Systematic Name
34	Temperature Optimum [°C]
2	Temperature Range [°C]
24	Temperature Stability [°C]
17	Turnover Number [1/s]

General Stability

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General Stability on EC 2.7.1.78 - polynucleotide 5'-hydroxyl-kinase

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GENERAL STABILITY

ORGANISM

UNIPROT

LITERATURE

ATP protects against heat denaturation and tryptic digestion

Rattus norvegicus

-

81073

bovine serum albumin and dithiothreitol stabilize the enzyme during activity assay

Homo sapiens

-

642021

freezing and thawing reduces activity after 1 cycle by 60%

2 entries

greatest protection from heat inactivation with substrates for the kinase, e.g. thymus DNA and ribosomal RNA, both micrococcal nuclease-treated and 3'-AMP

Escherichia phage T2

-

642006

high ionic strength, 0.1 M KCl, spermine, ATP and thymidine 3'-monophosphate stabilize the oligomeric structure

Tequatrovirus T4

-

642011

high ionic strength, e.g. 0.1 M KCl, spermine, ATP and thymidine 3'-monophosphate are essential to stabilize the enzyme in an oligomeric state

Tequatrovirus T4

-

642011

protection against heat inactivation by ATP or 5'-OH-DNA at low and moderately high NaCl concentrations

Rattus norvegicus

-

642007

freezing and thawing reduces activity after 1 cycle by 60%

Tequatrovirus T4

-

642004, 642005

freezing and thawing reduces activity after 1 cycle by 60%

Escherichia phage T2

-

642004, 642005