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1.14.13.39: nitric-oxide synthase (NADPH)

This is an abbreviated version!
For detailed information about nitric-oxide synthase (NADPH), go to the full flat file.

Word Map on EC 1.14.13.39

Reaction

2 L-arginine + 2 NADPH + 2 H+ + 2 O2 = 2 Nomega-hydroxy-L-arginine + 2 NADP+ + 2 H2O

Synonyms

bacterial nitric oxide synthase, bacterial nitric-oxide synthase, bNOS, bsNOS, cb-NOS, cytokine inducible NOS, DNOS, e-NOS, EC-NOS, endothelial nitric oxide synthase, endothelial nitric-oxide synthase, endothelial NO synthase, endothelial NOS, endothelium-derived relaxation factor-forming enzyme, endothelium-derived relaxing factor synthase, eNOS, i-NOS, inducible nitric oxide synthase, inducible nitric-oxide synthase, inducible NO synthase, inducible NOS, iNOS, mitochondrial NO synthase, mitochondrial-specific nitric oxide synthase, mtNOS, n-NOS, NADPH diaphorase, NADPH-d, NADPH-diaphorase, NADPHd, neuronal nitric oxide synthase, neuronal nitric-oxide synthase, neuronal NO synthase, neuronal NOS, nicotinamide adenine dinucleotide phosphate-diaphorase, nitric oxid synthase, nitric oxide synthase, nitric oxide synthase-like protein, nitric oxide synthetase, nitric-oxide synthase, nNOS, nNOSalpha, NO synthase, NO synthase type I, NO synthase type II, NO synthase type III, NO-synthase, NOS, NOS I, NOS-2, NOS1, NOS2, NOS3, synthetase, nitric oxide

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.39 nitric-oxide synthase (NADPH)

Purification

Purification on EC 1.14.13.39 - nitric-oxide synthase (NADPH)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2',5'-ADP-affinity and anion exchange chromatography
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2',5'-ADP-agarose affinity chromatography
affinity and size exclusion chromatography
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affinity chromatography
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ammonium sulfate precipitation (30% saturation), 2',5'-ADP-affinity chromatography
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cerebellum wild-type and recombinant brain enzyme
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dimeric enzyme and subunits
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from interferon-gamma- and lipopolysaccharide-activated macrophage
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gel filtration chromatography
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heterodimer
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inducible form from macrophage
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native enzyme from axenic amastigotes by 2',5'-ADP affinity chromatography to homogeneity
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native enzyme from platelets activated by acetylsalicylic acid, by solubilization with Triton X-100, anion exchange chromatography, and gel filtration, to homogeneity
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native enzyme from tilapia, by 2',5'-ADP affinity chromatography, ion exchange chromatography, and gel filtration
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native rat neuronal NOS reductase domain (nNOSred) and the R1400E variant
Ni Sepharose column chromatography, 2',5'-ADP Sepharose column chromatography, and Superdex 200 gel filtration
Ni-NTA column chromatography, gel filtration
Ni-NTA Sepharose column chromatography and 2'-5' ADP Sepharose resin column chromatography
partial
partial, 2',5'-ADP-agarose affinity chromatography
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partially by mitochondria preparation
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recombinant bNOS by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
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recombinant endothelial isoform from human embryonic kidney cells
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recombinant endothelial isoform III from insect cells
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recombinant eNOS from Escherichia coli by 2',5'-ADP affinity chromatography
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recombinant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
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recombinant enzyme oxygenase domain from Escherichia coli in the absence of both Arg and tetrahydrobiopterin
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recombinant from Escherichia coli
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recombinant from Pichia pastoris
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recombinant His-tagged construct dNOSr from Escherichia coli strain BL21 by nickel affinity and calmodulin affinity chromatography
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recombinant His-tagged dNOS from Escherichia coli by nickel affinity and anion exchange chromatography in the presence of L-Arg and H4B
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recombinant His-tagged or GST-tagged truncated nNOS, comprising residues 1-198, from Escherichia coli by nickel affinity or glutathione affinity chromatography, respectively
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recombinant His-tagged wild-type enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant truncation mutant from Escherichia coli strain BL21(DE3) by sequential 2',5'-ADP and calmodulin affinity chromatography
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recombinant His6-tagged bNOS from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
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recombinant His6-tagged soluble enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
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recombinant neuronal enzyme from HEK-293 cells by 2',5'-ADP affinity chromatography to about 90% purity
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recombinant nNOS from Escherichia coli in absence of tetrahydrobiopterin
recombinant wild-type of inducible liver isoform from Escherichia coli with and without His-tag, requires inclusion of tetrahydrobiopterin in purification buffer
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sequential affinity chromatography on 2',5'-ADP-agarose and calmodulin Sepharose 4B
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sequential anion-exchange-, affinity chromatogaphy
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wild-type and mutants from insect cells
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wild-type and natural mutant, recombinant from Escherichia coli and insect cells
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wild-type recombinant from insect cells
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