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1.14.16.4: tryptophan 5-monooxygenase

This is an abbreviated version!
For detailed information about tryptophan 5-monooxygenase, go to the full flat file.

Word Map on EC 1.14.16.4

Reaction

L-tryptophan
+
a 5,6,7,8-tetrahydropteridine
 +
O2
=
5-hydroxy-L-tryptophan
 +
a 4a-hydroxy-5,6,7,8-tetrahydropteridine

Synonyms

hTPH2, indoleacetic acid-5-hydroxylase, L-tryptophan 5-hydroxylase, L-tryptophan hydroxylase, oxygenase, tryptophan 5-mono-, peripheral tryptophan hydroxylase, PTMO, putative tryptophan monooxygenase, TH2, TPH, TPH-1, TPH-2, TPH1, TPH2, TRpOH, tryptophan 5-hydroxylase, tryptophan 5-monooxygenase, tryptophan hydroxylase, tryptophan hydroxylase 1, tryptophan hydroxylase 2, tryptophan hydroxylase I, tryptophan hydroxylase isoform 1, tryptophan hydroxylase isoform 2, tryptophan hydroxylase isoform2, tryptophan hydroxylase type I, tryptophan hydroxylase-1, tryptophan hydroxylase-2, tryptophan-5-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.16 With reduced pteridine as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.16.4 tryptophan 5-monooxygenase

General Stability

General Stability on EC 1.14.16.4 - tryptophan 5-monooxygenase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
3-amino-2-benzyl-7-nitro-4-(2-quinolyl)-1,2-dihydroisoquinolin-1-one strongly stabilizes the protein during denaturation by heat. Half-denaturation temperature increases from 55.5°C to 64.4°C in the presence of 0.1 mM 3-amino-2-benzyl-7-nitro-4-(2-quinolyl)-1,2-dihydroisoquinolin-1-one.
-
catalase at a concentration of more than 1 mg/ml is required for stability during reaction at aerobic conditiones
-
catalase is necessary to protect the enzyme during purification
-
EDTA stabilizes
EDTA: 0.05 mM stabilizes
ethylene glycol, 50%, stabilizes
-
Fe2+ protects against inactivation by H2O2 in absence of catalase
-
glycerol stabilizes
-
glycerol, 10% stabilizes
-
high-affinity binding of purified PKA-phosphorylated TPH2 bound to the 14-3-3 proteins gamma , epsilon and BMH1 causes increased enzyme stability
in the presence of L-Phe, the enzyme dimer displays significantly increased half-life
-
L-tryptophan stabilizes
-
NaCl: 1 M, in addition to other stabilizing agents brings a great improvement in stabilization
-
The half-life of the protein is 30 min at 37°C, while the N-terminal and the N- and C-terminal truncated mutants exhibit half-lives of 203 min and 147 min, respectively
-
tryptophan and glycerol stabilize
-
Tween 20 stabilizes
Tween 20: 0.06% stabilizes