2.3.3.13: 2-isopropylmalate synthase

This is an abbreviated version!
For detailed information about 2-isopropylmalate synthase, go to the full flat file.

Reaction

Synonyms

2-isopropylmalate synthase 1, chloroplastic, 2-isopropylmalate synthase 2, chloroplastic, 3-hydroxy-4-methyl-3-carboxy-pentanoate 2-oxo-3-methyl-butanoate lyase (CoA-acetylating), 3-hydroxy-4-methyl-3-carboxyvalerate 2-oxo-3-methyl-butyrate lyase (CoA-acetylating), alpha-IPM synthase, Alpha-IPM synthetase, alpha-IPMS, alpha-IPMS-14CR, alpha-IPMS-2CR, Alpha-isopropylmalate synthase, alpha-isopropylmalate synthase I, alpha-isopropylmalate synthase II, alpha-isopropylmalate synthetase, alpha-isopropylmalic synthetase, BatIMS, EC 4.1.3.12, IPM synthase, IPM-synthase, IPMS, IPMS1, IPMS2, IPMS3, isopropylmalate synthase, isopropylmalate synthase 1, isopropylmalate synthetase, LEU4, LEU9, leuA, Mj1195, MtalphaIPMS, MtIPMS, NmeIPMS, Os11g04670, Os12g04440, OsIPMS1, OsIPMS2, ROL17, synthase, 2-isopropylmalate, synthase, alpha-isopropylmalate, TT_C0849

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.3 Acyl groups converted into alkyl groups on transfer

                2.3.3.13 2-isopropylmalate synthase

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Results	in table
1	Activating Compound
38019	AA Sequence
11	Application
1	CAS Registry Number
24	Cloned(Commentary)
3	Cofactor
5	Crystallization (Commentary)
3	Expression
15	General Information
1	General Stability
1	IC50 Value
112	Inhibitors
15	kcat/KM [mM/s]
46	Ki Value [mM]
116	KM Value [mM]
12	Localization
46	Metals/Ions
31	Molecular Weight [Da]
76	Natural Substrates/ Products (Substrates)
589	Organism
8	Pathway
22	PDB ID
25	pH Optimum
3	pH Range
8	pH Stability
1	Posttranslational Modification
50	Protein Variants
27	Purification (Commentary)
2	Reaction
9	Reaction Type
116	Reference
1	Renatured (Commentary)
20	Source Tissue
15	Specific Activity [micromol/min/mg]
8	Storage Stability
224	Substrates and Products (Substrate)
23	Subunits
116	Synonyms
1	Systematic Name
14	Temperature Optimum [°C]
2	Temperature Range [°C]
11	Temperature Stability [°C]
75	Turnover Number [1/s]

Natural Substrates Products

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of the Enzyme Summary Page.

Natural Substrates Products on EC 2.3.3.13 - 2-isopropylmalate synthase

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

47 entries

acetyl-CoA + 3-methyl-2-oxobutanoate + H2O

(2S)-2-isopropylmalate + CoA

16 entries

acetyl-CoA + alpha-ketoisovalerate + H2O

alpha-isopropylmalate + CoA

2 entries

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Alcaligenes viscolactis

-

first enzyme in biosynthesis of L-Leu

636533

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Arabidopsis thaliana

Q9C550, Q9FG67, Q9FN52

first enzyme in biosynthesis of L-Leu

636536

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Arabidopsis thaliana IMS 1

Q9C550

first enzyme in biosynthesis of L-Leu

636536

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Arabidopsis thaliana IMS 2

Q9FN52

first enzyme in biosynthesis of L-Leu

636536

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Arabidopsis thaliana IMS 3

Q9FG67

first enzyme in biosynthesis of L-Leu

636536

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Bacillus subtilis

-

first enzyme in biosynthesis of L-Leu

636533

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Bacteroides fragilis

-

involved in Leu biosynthesis

636509, 636512

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Bradyrhizobium japonicum

-

first enzyme in biosynthesis of L-Leu

636533

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Candida maltosa

-

-

5664

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Cereibacter sphaeroides

-

first enzyme in biosynthesis of L-Leu

636533

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Chromatium sp.

-

first enzyme in biosynthesis of L-Leu

636533

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Chromatium sp. D

-

first enzyme in biosynthesis of L-Leu

636533

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Corynebacterium glutamicum

-

involved in Leu biosynthesis

636528

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Corynebacterium glutamicum

-

key enzyme in biosynthesis of Leu

636528

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Cupriavidus necator

-

first enzyme in biosynthesis of L-Leu

636516

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Cupriavidus necator

-

the enzyme is regulated by the level of Leu

636508

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Cupriavidus necator

-

involved in Leu biosynthesis

636508

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Cupriavidus necator H 16

-

the enzyme is regulated by the level of Leu

636508

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Cupriavidus necator H 16

-

involved in Leu biosynthesis

636508

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Cupriavidus necator H 16

-

first enzyme in biosynthesis of L-Leu

636516

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Escherichia coli

-

first enzyme in biosynthesis of L-Leu

636533

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Escherichia coli B / ATCC 11303

-

first enzyme in biosynthesis of L-Leu

636533

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Hydrogenomonas sp.

-

first enzyme in biosynthesis of L-Leu

636523, 636533

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Hydrogenomonas sp.

-

repression by Leu

636523

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Hydrogenomonas sp. H16

-

first enzyme in biosynthesis of L-Leu

636523

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Hydrogenomonas sp. H16

-

repression by Leu

636523

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Klebsiella aerogenes

-

first enzyme in biosynthesis of L-Leu

636533

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Methylorubrum extorquens

-

first enzyme in biosynthesis of L-Leu

636533

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Micrococcus luteus

-

first enzyme in biosynthesis of L-Leu

636533

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Micrococcus sp.

-

first enzyme in biosynthesis of L-Leu

636533

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Moorella thermoacetica

-

repression by Leu

636512

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Moorella thermoacetica

-

involved in Leu biosynthesis

636512

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Mycobacterium tuberculosis

-

first enzyme in biosynthesis of L-Leu

636535

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Neurospora crassa

-

first enzyme in biosynthesis of L-Leu

636525

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Paenibacillus polymyxa

-

first enzyme in biosynthesis of L-Leu

636533

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Prevotella ruminicola

-

involved in Leu biosynthesis

636509

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Prevotella ruminicola 23

-

involved in Leu biosynthesis

636509

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Proteus vulgaris

-

first enzyme in biosynthesis of L-Leu

636533

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Pseudomonas aeruginosa

-

first enzyme in biosynthesis of L-Leu

636533

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Pseudomonas fluorescens

-

first enzyme in biosynthesis of L-Leu

636533

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Rhodococcus opacus

-

first enzyme in biosynthesis of L-Leu

636533

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Saccharomyces cerevisiae

-

first enzyme in biosynthesis of L-Leu

636513, 636520, 636532, 636534

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Saccharomyces cerevisiae

-

the cytoplasmic form is involved in Leu biosynthesis

636507

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Saccharomyces cerevisiae 60615

-

first enzyme in biosynthesis of L-Leu

636520

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Saccharomyces cerevisiae SK101

-

first enzyme in biosynthesis of L-Leu

636513

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Salmonella enterica subsp. enterica serovar Typhimurium

-

first enzyme in biosynthesis of L-Leu

636524, 636533

-

-

?

2-oxo-3-methylbutanoate + acetyl-CoA + H2O

?

Salmonella enterica subsp. enterica serovar Typhimurium CV-19

-

first enzyme in biosynthesis of L-Leu

636524

-

-

?

acetyl-CoA + 3-methyl-2-oxobutanoate + H2O

(2S)-2-isopropylmalate + CoA

Leptospira biflexa

B0SN40

-

736457

-

-

?

acetyl-CoA + 3-methyl-2-oxobutanoate + H2O

(2S)-2-isopropylmalate + CoA

Methanocaldococcus jannaschii

-

-

735677

-

-

?

acetyl-CoA + 3-methyl-2-oxobutanoate + H2O

(2S)-2-isopropylmalate + CoA

Methanocaldococcus jannaschii

Q58595

the hydrolytic step is rate-determining

727047

-

-

?

acetyl-CoA + 3-methyl-2-oxobutanoate + H2O

(2S)-2-isopropylmalate + CoA

Methanocaldococcus jannaschii DSM 2661

Q58595

the hydrolytic step is rate-determining

727047

-

-

?

acetyl-CoA + 3-methyl-2-oxobutanoate + H2O

(2S)-2-isopropylmalate + CoA

Mycobacterium tuberculosis

-

-

702335, 735673, 735710

-

-

?

acetyl-CoA + 3-methyl-2-oxobutanoate + H2O

(2S)-2-isopropylmalate + CoA

Mycobacterium tuberculosis

P9WQB3

-

702277, 702904

-

-

?

acetyl-CoA + 3-methyl-2-oxobutanoate + H2O

(2S)-2-isopropylmalate + CoA

Mycobacterium tuberculosis

-

-

702904

-

-

?

acetyl-CoA + 3-methyl-2-oxobutanoate + H2O

(2S)-2-isopropylmalate + CoA

Mycobacterium tuberculosis

P9WQB3

-

735592, 736106

-

-

?

acetyl-CoA + 3-methyl-2-oxobutanoate + H2O

(2S)-2-isopropylmalate + CoA

Mycobacterium tuberculosis H37Rv

P9WQB3

-

702277, 702904

-

-

?

acetyl-CoA + 3-methyl-2-oxobutanoate + H2O

(2S)-2-isopropylmalate + CoA

Neisseria meningitidis

Q9JZG1

-

702097

-

-

?

acetyl-CoA + 3-methyl-2-oxobutanoate + H2O

(2S)-2-isopropylmalate + CoA

Oryza sativa Japonica Group

Q2QXY9, Q2RAP8

the enzyme catalyses the committed step of leucine biosynthesis

757963

-

-

?

acetyl-CoA + 3-methyl-2-oxobutanoate + H2O

(2S)-2-isopropylmalate + CoA

Saccharomyces cerevisiae

-

-

736032

-

-

?

acetyl-CoA + 3-methyl-2-oxobutanoate + H2O

(2S)-2-isopropylmalate + CoA

Saccharomyces cerevisiae BY4741

-

-

736032

-

-

?

acetyl-CoA + 3-methyl-2-oxobutanoate + H2O

(2S)-2-isopropylmalate + CoA

Solanum lycopersicum

K4C627, K4CJ56

-

737033

-

-

?

acetyl-CoA + 3-methyl-2-oxobutanoate + H2O

(2S)-2-isopropylmalate + CoA

Solanum pennellii

A0A0G2T6D7

-

737033

-

-

?

acetyl-CoA + 3-methyl-2-oxobutanoate + H2O

(2S)-2-isopropylmalate + CoA

Thermus thermophilus

Q72JC9

enzyme of the branched-chain amino acid biosynthetic gene cluster. The enzyme is involved in biosynthesis of leucine and isoleucine

755968

-

-

?

acetyl-CoA + alpha-ketoisovalerate + H2O

alpha-isopropylmalate + CoA

Mycobacterium tuberculosis

-

first step of leucine biosynthesis

657457, 658985

-

-

?

acetyl-CoA + alpha-ketoisovalerate + H2O

alpha-isopropylmalate + CoA

Mycobacterium tuberculosis H37Rv

-

first step of leucine biosynthesis

657457

-

-

?