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2.3.3.13: 2-isopropylmalate synthase

This is an abbreviated version!
For detailed information about 2-isopropylmalate synthase, go to the full flat file.

Word Map on EC 2.3.3.13

Reaction

acetyl-CoA
+
3-methyl-2-oxobutanoate
 +
H2O
=
(2S)-2-isopropylmalate
 +
CoA

Synonyms

2-isopropylmalate synthase 1, chloroplastic, 2-isopropylmalate synthase 2, chloroplastic, 3-hydroxy-4-methyl-3-carboxy-pentanoate 2-oxo-3-methyl-butanoate lyase (CoA-acetylating), 3-hydroxy-4-methyl-3-carboxyvalerate 2-oxo-3-methyl-butyrate lyase (CoA-acetylating), alpha-IPM synthase, Alpha-IPM synthetase, alpha-IPMS, alpha-IPMS-14CR, alpha-IPMS-2CR, Alpha-isopropylmalate synthase, alpha-isopropylmalate synthase I, alpha-isopropylmalate synthase II, alpha-isopropylmalate synthetase, alpha-isopropylmalic synthetase, BatIMS, EC 4.1.3.12, IPM synthase, IPM-synthase, IPMS, IPMS1, IPMS2, IPMS3, isopropylmalate synthase, isopropylmalate synthase 1, isopropylmalate synthetase, LEU4, LEU9, leuA, Mj1195, MtalphaIPMS, MtIPMS, NmeIPMS, Os11g04670, Os12g04440, OsIPMS1, OsIPMS2, ROL17, synthase, 2-isopropylmalate, synthase, alpha-isopropylmalate, TT_C0849

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.3 Acyl groups converted into alkyl groups on transfer
                2.3.3.13 2-isopropylmalate synthase

Cloned

Cloned on EC 2.3.3.13 - 2-isopropylmalate synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
construction of a Leu4'-'lacZ fusion. The Leu4 gene encodes 2 forms: a short cytoplasmic form and a long form that is targeted to the mitochondria
-
construction of three knock-out mutants, two carrying a deletion in YOR108w or in Leu4 ORF, and one carrying both the deletions
-
expressed as His-tag fusion protein in Escherichia coli BL21(DE3)
-
expressed in a Schizosaccharomyces pombe leu3DELTA deletion strain
-
expressed in Escherichia coli BL21 Rosetta cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) Star cells
expressed in Escherichia coli BL21(DE3)pLysS cells
expression in Escherichia coli
expression in Escherichia coli and Bacillus subtilis
-
H37Rv leuA gene with and without the tandem repeats is cloned by PCR and expressed in an Escherichia coli host. The 57-bp tandem repeats located in the leuA gene code for the alpha-isopropylmalate synthase. Deletion of the repeats has no detectable effect on leuA expression level or the general properties of the enzyme product
-
into the vector pET-151 for expression in Escherichia coli BL21DE3 Star cells
into the vector pET28a for expression in Escherichia coli BL21DE3 pLysS cells
the coding sequences of alpha-IPMS-2CR and alpha-IPMS-14CR are cloned into the vector pET15b for expression in Escherichia coli BL21DE3 cells
truncated protein E365Term is expressed in Escherichia coli BL21(DE3) Star cells and in Escherichia coli strain BW25113 lacking the alpha-isopropylmalate synthase gene
truncated protein LeuA425 is expressed in Escherichia coli Rosetta 2 cells and in Escherichia coli strain BW25113 lacking the alpha-isopropylmalate synthase gene