overexpression of Daucus carota enzyme in Arabidopsis thaliana plant mitochondria improves the ability of the plants to grow in phosphorus limited soil due to enhanced citrate excretion from the roots
overexpression of Daucus carota enzyme in Arabidopsis thaliana plant mitochondria improves the ability of the plants to grow in phosphorus limited soil due to enhanced citrate excretion from the roots
citrate synthase deletion mutant is highly attenuated in virulence. The mutant has lost all its enzymic activity and shows a 10fold reduction in vir gene expression. The mutation results in only a minor reduction in growth rate in a glucose-salts medium, and the reduced growth rate is not responsible for the attenuated virulence
construction of mutants with reduced enzymic activity by localized random PCR mutagenesis. In symbiosis with alfalfa, alfalfa plants form fully effective nodules when infected with Sinorhizobium meliloti mutants having as low as 7% of citrate synthase activity. Mutants with about 3% of wild-type citrate synthase activity form nodules with lower nitrogenase activity and a mutant with 0.5% of wild-type activity forms Fix-nodules
overexpression of Daucus carota enzyme in Arabidopsis thaliana plant mitochondria improves the ability of the plants to grow in phosphorus limited soil due to enhanced citrate excretion from the roots
citrate synthase deletion mutant is highly attenuated in virulence. The mutant has lost all its enzymic activity and shows a 10fold reduction in vir gene expression. The mutation results in only a minor reduction in growth rate in a glucose-salts medium, and the reduced growth rate is not responsible for the attenuated virulence
over-expression of citrate syntase gltA or phosphoenolpyruvate carboxylase ppc increases the maximum cell dry weight by 23% and 91% resp. No acetate excretion is detected at these increased cell densities