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2.7.7.48: RNA-directed RNA polymerase

This is an abbreviated version!
For detailed information about RNA-directed RNA polymerase, go to the full flat file.

Word Map on EC 2.7.7.48

Reaction

nucleoside triphosphate
+
RNAn
=
diphosphate
 +
RNAn+1

Synonyms

111 kDa protein, 180 kDa protein, 182 kDa protein, 183 kDa protein, 186 kDa protein, 216.5 kDa protein, 2A protein, 3CD protein, 3D pol, 3D polymerase, 3Dpol, 3Dpol-like protein, 3P complex, 69.6 kDa protein, core protein, core protein VP1, CVB3 RdRp, D-elp1, DENV 3 polymerase, DENV 3 RdRp, DENV RdRp, ego-1, ego-11, FluPol, FMDV 3D, GhRdRP, HC-J4 NS5BDELTA21, HCN NS5B protein, HCV NS5B, HCV NS5B polymerase, HCV RdRp, hepatitis C virus polymerase, HRV16 3Dpol, influenza polymerase PA, inner layer protein VP1, JEV NS5, JEV NS5 protein, JEV RdRp, jRdRp, L protein, L/P RdRP, large structural protein, M1 phosphoprotein, MOP1, More, NgRDR1, NIB, nonstructural phosphoprotein, nonstructural protein, nonstructural protein 12, nonstructural protein 5B, NS5, NS5 polymerase, NS5 protein, NS5 RdRp, NS5B, NS5B enzyme, NS5B polymerase, NS5B protein, NS5B RdRp, NS5B RNA-dependent RNA polymerase, NS5b-directed RNA polymerase, NS7, nsp12, nsp4, nucleocapsid phosphoprotein, nucleotidyltransferase, ribonucleate, RNA-dependent, ORF1, ORF1A, ORF1B, OsRDR6, P protein, P180, P3D, P66, P70, P88 protein, p92, PB1, PB1 proteins, PB2, PB2 proteins, Phage f2 replicase, phi6, phi6 polymerase, picornaviral 3D polymerase, plant-encoded RNA-dependent RNA polymerase 1, Pol, Pol IV, polymerase 3Dpol, polymerase acidic protein, polymerase basic 1 protein, polymerase L, protein 3Dpol, proteins PB1, proteins, PB 2, proteins, specific or class, lambda3, of reovirus, proteins, specific or class, PB 1, proteins, specific or class, PB 2, Q-beta replicase, Qbeta replicase, Qbeta-replicase, RDR, RDR1, RDR1a, RDR1b, RDR1c, RDR1c2, RDR2, RDR6, RDRP, replicase, phage f2, replicase, Qbeta, ribonucleic acid replicase, ribonucleic acid-dependent ribonucleate nucleotidyltransferase, ribonucleic acid-dependent ribonucleic acid polymerase, ribonucleic replicase, ribonucleic synthetase, RNA dependent RNA polymerase, RNA nucleotidyltransferase (RNA-directed), RNA polymerase, RNA polymerase IV, RNA replicase, RNA synthetase, RNA transcriptase, RNA-binding protein, RNA-dependent ribonucleate nucleotidyltransferase, RNA-dependent RNA polymerase, RNA-dependent RNA polymerase 1, RNA-Dependent RNA Polymerase 2, RNA-dependent RNA polymerase 6, RNA-dependent RNA polymerase NIb, RNA-dependent RNA polymerase NS5B, RNA-dependent RNA polymerases, RNA-dependent RNA replicase, RNA-dependent RNA-polymerase, RNA-directed RNA polymerase, RNA-directed RNA polymerase L, RNAdependent RNA polymerase, rrf-1, RRF-3, RrpC, RSV RdRp, SARS-CoV-2 RdRp, SARS-CoV-2 RNA polymerase, SARSCoV-2 polymerase, SARSCoV-2-core polymerase complex, self-priming RdRp, sigma NS protein, transcriptase, VP1, VP1 protein, WNV NS5

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.48 RNA-directed RNA polymerase

Purification

Purification on EC 2.7.7.48 - RNA-directed RNA polymerase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
a hexahistidine affinity-tagged NS5B fusion protein expressed with recombinant baculoviruses in insect cells
-
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Superdex 200 gel filtration
-
C-terminally truncated enzyme form NS5B(DELTA21) expressed in Escherichia coli
-
chelating Sepharose flast flow resin chromatography and Superdex 200 gel filtration
-
enzymatically active full-length recombinant JEV NS5 protein with a hexahistidine tag at the N-terminus
-
enzyme expressed in Escherichia coli
-
full-length enzyme and C-terminal hydrophobic domain deletion mutant expressed in Escherichia coli or in Sf9 cells
-
full-length enzyme and truncated enzyme forms resulting from deletions of 24, 36, 65 or 82 amino acid residues at the C terminal, with C-terminal hexahistidine tag
-
HisTrap HP column chromatography, 15S column chromatography, ultrafiltration, and Superdex 200 gel filtration
Q6DLV0
HisTrap HP column chromatography, Source 15S column chromatography, and gel filtration
-
Ni2+-Sepharose column chromatography
NS5B-hexahistidine fusion protein expressed with recombinant baculoviruses in insect Sf9 cells
-
partial
purification is vitiated by its great instability
-
purified from Escherichia coli to near homogeneity. When the 21 amino acids from the C-terminus of the protein are deleted, an inclusion body is not formed and a better purification yield is achieved
-
putative RNA-dependent RNA polymerase (RdRp) domain cloned and expressed in Escherichia coli
-
recombinant C-terminally His-tagged RdRp as the C-terminal domain of nonstructural protein 9 from Escherichia coli strain BL21 (DE3) by cobalt affinity chromatography and gel filtration
-
recombinant C-terminally HIs6-tagged enzyme from Escherichia coli by nickel affinity chromatography
recombinant enzyme expressed in Escherichia coli
-
recombinant enzyme from Escherichia coli by affinity chromatography and gel filtration
recombinant enzyme P2 from Escherichia coli strain BL21 (DE3) by anion exchange chromatography, gel filtration, dialysis, and ultrafiltration
recombinant enzyme, wild-type and mutant enzymes, expressed in Escherichia coli
-
recombinant FLAG-tagged D-elp1 from Spodoptera frugiperda Sf9 cells by affinity chromatography. His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography
-
recombinant full-length enzyme expressed in baculovirus Sf9 insect cells
-
recombinant His-tagged enzyme variants from Escherichia coli by nickel affinity chromatography
recombinant His-tagged RdRp_2 from Escherichia coli strain M15 by nickel affiniity chromatography
-
recombinant His-tagged subunits PA and PB1 from Escherichia coli by nickel affinity chromatography
recombinant His6-tagged NS5B from Hep-G2 cells by nickel affinity chromatography
recombinant N-terminally GST-tagged enzyme from Escherichia coli strain C41(DE3) by glutathione affinity chromatography and gel filtration
recombinant viral His18-tagged enzyme components from Sf21AE and TN5 insect cells
-
recombinant wild-type and mutant His-tagged enzymes from Escherichia coli strain BL21(DE3) or recombinant soluble His6-tagged wild-type enzyme from High Five insect cells by nickel affinity chromatgraphy to homogeneity
-
Sepharose SPHP XK16/20 column chromatography, Q20 column chromatography, Q5 column chromatography, and UNO column chromatography
-
soluble Hepatitis C virus NS5B in the glutathione S-transferase-fused form NS5Bt, expressed in Escherichia coli
-
SP-Sepharose column chromatography
-
Talon IMAC resin column chromatography
-
three different recombinant forms of DENV-2 RdRp as either N- or C-terminally His-tagged fusions, or without tag from Escherichia coli strain s Rosetta (DE3) and BL21 (DE3) to homogeneity by nickel affinity and anion exchange chromatography, followed by gel filtration and ultracentrifugation