1.5.1.40: 8-hydroxy-5-deazaflavin:NADPH oxidoreductase
This is an abbreviated version!
For detailed information about 8-hydroxy-5-deazaflavin:NADPH oxidoreductase, go to the full flat file.
Word Map on EC 1.5.1.40
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1.5.1.40
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methanogen
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archaea
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hydride
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8-hydroxy-5-deazaflavins
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methanococcus
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hydrogenase
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vannielii
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thermoautotrophicum
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methanobacterium
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methanobrevibacter
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griseus
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methanogenesis
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stereochemical
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smithii
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si-face
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methane
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fulgidus
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ch4
- 1.5.1.40
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methanogen
- archaea
-
hydride
- 8-hydroxy-5-deazaflavins
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methanococcus
- hydrogenase
- vannielii
- thermoautotrophicum
-
methanobacterium
-
methanobrevibacter
- griseus
-
methanogenesis
-
stereochemical
- smithii
-
si-face
- methane
- fulgidus
- ch4
Reaction
Synonyms
5-deazaflavin-NADP+ reductase, 8-hydroxy-5-deazaflavin-dependent NADP+ reductase, 8-OH-5-deazaflavin:NADPH oxidoreductase, 8-OH-5dFl:NADPH oxidoreductase, AF0892, F420-dependent NADP oxidoreductase, F420-dependent NADP reductase, F420-dependent NADP+ oxidoreductase, F420:NADPH oxidoreductase, F420H2:NADP oxidoreductase, F420H2:NADP+ oxidoreductase, Fno, Msm_0049, NADP+:F420 oxidoreductase, Tfu-FNO, Tfu_0970
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Substrates Products
Substrates Products on EC 1.5.1.40 - 8-hydroxy-5-deazaflavin:NADPH oxidoreductase
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REACTION DIAGRAM
5'-O-methyl-7,8-didemethyl-8-hydroxyflavin + NADPH + H+
8-hydroxypyrimido[4,5-b]-2,4-(3H,10H)-dione + NADP+
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the enzyme has an absolute requirement for both the 5-deazaflavin structure and the presence of an 8-hydroxy group in the substrate
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r
5-deaza-8-hydroxyisoalloxazine + NADPH + H+
8-hydroxypyrimido[4,5-b]-2,4-(3H,10H)-dione + NADP+
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the enzyme has an absolute requirement for both the 5-deazaflavin structure and the presence of an 8-hydroxy group in the substrate
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-
?
7,8-didemethyl-8-hydroxy-5-deazariboflavin 5'-phosphate + NADPH + H+
1-deoxy-1-(8-hydroxy-2,4-dioxo-1,3,4,5-tetrahydropyrimido[4,5-b]quinolin-10(2H)-yl)-5-O-phospho-D-ribitol + NADP+
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the enzyme has an absolute requirement for both the 5-deazaflavin structure and the presence of an 8-hydroxy group in the substrate
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?
reduced coenzyme F420 + 1-aminoethylnicotinamide
oxidized coenzyme F420 + ?
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-
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?
reduced coenzyme F420 + 1-benzyl-3-acetylpyridine
oxidized coenzyme F420 + ?
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-
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?
reduced coenzyme F420 + 1-benzylnicotinamide
oxidized coenzyme F420 + ?
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?
reduced coenzyme F420 + 1-ethylnicotinamide
oxidized coenzyme F420 + ?
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?
reduced coenzyme F420 + 1-hydroxyethylnicotinamide
oxidized coenzyme F420 + ?
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-
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?
reduced coenzyme F420 + 1-hydroxypropylnicotinamide
oxidized coenzyme F420 + ?
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-
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?
reduced coenzyme F420 + 1-propylnicotinamide
oxidized coenzyme F420 + ?
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?
8-hydroxy-5-deazaflavin + NADPH + H+
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the kcat value of the forward reaction is 24 times greater than that of the reverse reaction, thus the production of NADPH at pH 7.0 is more favorable than its consumption
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r
1,5-dihydro-8-hydroxy-5-deazaflavin + NADP+
8-hydroxy-5-deazaflavin + NADPH + H+
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the kcat value of the forward reaction is 24 times greater than that of the reverse reaction, thus the production of NADPH at pH 7.0 is more favorable than its consumption
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r
? + NADP+
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-
-
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?
5-deaza-8-hydroxy-10-methylisoalloxazine + NADPH + H+
? + NADP+
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-
-
-
?
reduced coenzyme F0 + NADP+
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i.e. 7,8-didemethyl-8-hydroxy-5-deazariboflavin. The enzyme has an absolute requirement for both the 5-deazaflavin structure and the presence of an 8-hydroxy group in the substrate
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-
?
coenzyme F0 + NADPH + H+
reduced coenzyme F0 + NADP+
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i.e. 7,8-didemethyl-8-hydroxy-5-deazariboflavin. The enzyme has an absolute requirement for both the 5-deazaflavin structure and the presence of an 8-hydroxy group in the substrate
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-
?
coenzyme F0 + NADPH + H+
reduced coenzyme F0 + NADP+
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i.e. 7,8-didemethyl-8-hydroxy-5-deazariboflavin. The enzyme has an absolute requirement for both the 5-deazaflavin structure and the presence of an 8-hydroxy group in the substrate
-
-
?
coenzyme F0 + NADPH + H+
reduced coenzyme F0 + NADP+
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i.e. 7,8-didemethyl-8-hydroxy-5-deazariboflavin. The enzyme has an absolute requirement for both the 5-deazaflavin structure and the presence of an 8-hydroxy group in the substrate
-
-
?
coenzyme F0 + NADPH + H+
reduced coenzyme F0 + NADP+
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i.e. 7,8-didemethyl-8-hydroxy-5-deazariboflavin. The enzyme has an absolute requirement for both the 5-deazaflavin structure and the presence of an 8-hydroxy group in the substrate
-
-
?
reduced coenzyme F420 + NADP+
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the enzyme has an absolute requirement for both the 5-deazaflavin structure and the presence of an 8-hydroxy group in the substrate. No activity with NAD+
-
-
?
coenzyme F420 + NADPH + H+
reduced coenzyme F420 + NADP+
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the enzyme has an absolute requirement for both the 5-deazaflavin structure and the presence of an 8-hydroxy group in the substrate. No activity with NAD+
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-
?
coenzyme F420 + NADPH + H+
reduced coenzyme F420 + NADP+
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the main function of this oxidoreductase is probably to provide cells with reduced 8-hydroxy-5-deazaflavin to be used in specific reduction reactions
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-
?
coenzyme F420 + NADPH + H+
reduced coenzyme F420 + NADP+
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the enzyme has an absolute requirement for both the 5-deazaflavin structure and the presence of an 8-hydroxy group in the substrate. No activity with NAD+
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-
r
coenzyme F420 + NADPH + H+
reduced coenzyme F420 + NADP+
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the enzyme has an absolute requirement for both the 5-deazaflavin structure and the presence of an 8-hydroxy group in the substrate. No activity with NAD+
-
-
?
coenzyme F420 + NADPH + H+
reduced coenzyme F420 + NADP+
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the enzyme has an absolute requirement for both the 5-deazaflavin structure and the presence of an 8-hydroxy group in the substrate. No activity with NAD+
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-
?
reduced coenzyme F420 + NADP+
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-
-
?
oxidized coenzyme F420 + NADPH + H+
reduced coenzyme F420 + NADP+
-
-
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?
reduced coenzyme F420 + NADP+
coenzyme F420 + NADPH + H+
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direct hydride transfer process, A side-specific, with respect to the prochiral center C5 of the dihydro-8-hydroxy-5-deazaflavin cofactor
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-
?
reduced coenzyme F420 + NADP+
coenzyme F420 + NADPH + H+
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-
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r
reduced coenzyme F420 + NADP+
oxidized coenzyme F420 + NADPH + H+
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-
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r
reduced coenzyme F420 + NADP+
oxidized coenzyme F420 + NADPH + H+
of the two substrates NADP+ has to bind first, the binding being associated with an induced fit. The stereochemical analysis of the hydrode transfer leads to the conclusion that the observed orientation of the Si-face of coenzyme F420 towards the Si-face of NADP+ allows only the transfer of the proS hydrogen at C5 to the proS position at C4 and vice versa
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-
?
reduced coenzyme F420 + NADP+
oxidized coenzyme F420 + NADPH + H+
the enzyme is Si face specific with respect to C5 of reduced coenzyme F420 and Si face specific with respect to C4 of NADP+. The enzyme is specific for both coenzyme F420 and NADP+/NADPH
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r
reduced coenzyme F420 + NADP+
oxidized coenzyme F420 + NADPH + H+
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r
reduced coenzyme F420 + NADP+
oxidized coenzyme F420 + NADPH + H+
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r
reduced coenzyme F420 + NADP+
oxidized coenzyme F420 + NADPH + H+
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-
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r
reduced coenzyme F420 + NADP+
oxidized coenzyme F420 + NADPH + H+
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-
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r
reduced coenzyme F420 + NADP+
oxidized coenzyme F420 + NADPH + H+
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-
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r
reduced coenzyme F420 + NADP+
oxidized coenzyme F420 + NADPH + H+
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-
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r
reduced coenzyme F420 + NADP+
oxidized coenzyme F420 + NADPH + H+
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-
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r
reduced coenzyme F420 + NADP+
oxidized coenzyme F420 + NADPH + H+
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-
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r
reduced coenzyme F420 + NADP+
oxidized coenzyme F420 + NADPH + H+
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-
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r
reduced coenzyme F420 + NADP+
oxidized coenzyme F420 + NADPH + H+
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-
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r
reduced coenzyme F420 + NADP+
oxidized coenzyme F420 + NADPH + H+
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-
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r
reduced coenzyme F420 + NADP+
oxidized coenzyme F420 + NADPH + H+
the enzyme exhibits a sequential kinetic mechanism
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r
reduced coenzyme F420 + NADP+
oxidized coenzyme F420 + NADPH + H+
the enzyme exhibits a sequential kinetic mechanism
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r
reduced coenzyme F420 + NADP+
oxidized coenzyme F420 + NADPH + H+
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r
reduced coenzyme F420 + NADP+
oxidized coenzyme F420 + NADPH + H+
FNO catalyzes the NADP+ reduction more efficiently compared to NADPH oxidation
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r
?
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F420 and the F420 redox moiety, FO, are phenolic 5-deazaflavin cofactors that complement nicotinamide and flavin redox coenzymes in biochemical oxidoreductases and photocatalytic systems. Specifically, these 5-deazaflavins lack the single electron reactivity with O2 of riboflavin-derived coenzymes (FMN and FAD), and, in general, have a more negative redox potential than NAD(P)+. A convenient synthesis of FO is achieved by improving the redox stability of synthetic intermediates containing a polar, electron-rich aminophenol fragment, Fno enzyme activity is restored with FO in the absence of F420, method optimization, overview
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?
additional information
?
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effects of side chain length of residue Il135 on the donor-acceptor distance between NADP+ and the F420 precursor, FO, overview
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?
additional information
?
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NADP+ binding site structure, overview. A F420-dependent enzyme
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?
additional information
?
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NADP+ binding site structure, overview. A F420-dependent enzyme
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?
additional information
?
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no activity of wild-type and mutants with 1-benzylnicotinic acid
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