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1.14.14.83: geraniol 8-hydroxylase

This is an abbreviated version!
For detailed information about geraniol 8-hydroxylase, go to the full flat file.

Word Map on EC 1.14.14.83

Reaction

geraniol
+
[reduced NADPH-hemoprotein reductase]
 +
O2
=
(6E)-8-hydroxygeraniol
 +
[oxidized NADPH-hemoprotein reductase]
 +
H2O

Synonyms

10-hydroxygeraniol oxidoreductase, CPR I, Cr10HGO, CYP76B10, CYP76B6, CYP76B6 protein, CYP76F45, EC 1.14.13.152, G10H, G8H, geraniol 10-hydroxylase, geraniol-10-hydroxylase, monoterpene hydroxylase, PgCYP76C9, PgG10H

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.83 geraniol 8-hydroxylase

Purification

Purification on EC 1.14.14.83 - geraniol 8-hydroxylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native cholate-solubilized G10H about 8.3fold from suspension cell membranes by anion exchange and hydroxyapatite chromatography, and 2-aminooctyl agarose and a second step of hydrophobic interaction chromatography to homogeneity. Separation from the NADPH-cytochrome P-450 reductase, EC 1.6.2.4
-
native enzyme about 12fold from membranes by anion exchange and omega-amino octyl agarose chromatography, followed by hydophobic interaction chromatography to homogeneity
-
solubilization by treatment with sodium cholate, Renex-30, or Lubrol-WX
-