Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.14.83: geraniol 8-hydroxylase

This is an abbreviated version!
For detailed information about geraniol 8-hydroxylase, go to the full flat file.

Word Map on EC 1.14.14.83

Reaction

geraniol
+
[reduced NADPH-hemoprotein reductase]
 +
O2
=
(6E)-8-hydroxygeraniol
 +
[oxidized NADPH-hemoprotein reductase]
 +
H2O

Synonyms

10-hydroxygeraniol oxidoreductase, CPR I, Cr10HGO, CYP76B10, CYP76B6, CYP76B6 protein, CYP76F45, EC 1.14.13.152, G10H, G8H, geraniol 10-hydroxylase, geraniol-10-hydroxylase, monoterpene hydroxylase, PgCYP76C9, PgG10H

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.83 geraniol 8-hydroxylase

Cloned

Cloned on EC 1.14.14.83 - geraniol 8-hydroxylase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning from a lambda-ZAPII library, recombinant expression in Saccharomyces cerevisiae strain YPH500 or in enzyme-deficient Catharathus roseus cell line MP183L, complementation
-
co-expression of G10H with the NADPH-cytochrome P450 reductase from Arabidopsis thaliana in Spodoptera frugiperda Sf9 cell microsomes using the baculovirus transfection method, quantitative expression analysis
co-overexpression of geraniol-10-hydroxylase and strictosidine synthase in Ophiorrhiza pumila
expression in Nothapodytes nimmoniana leaf
-
expression in Saccharomyces cerevisiae
expression in Spodoptera frugiperda SF9 cells, co-expression with NADPH:cytochrome P450 reductase NfCPR2 from Nothapodytes foetida and CPR2 from Arabidopsis thaliana
gene CrG10H, recombinant expression in transgenic Ophiorrhiza pumila hairy roots using Agrobacterium rhizogenes strain C58C1 for transfection
gene CsCPR I, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis and tree, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3). Only simultaneous incubation of the membrane-bound enzymes CsCYP76F45 and CsCPR I with the substrate geraniol and the coenzyme NADPH allows 8-hydroxygeraniol formation
gene CYP76B10, DNA and amino acid sequence determination and analysis, semi-quantitative RT-PCR expression analysis, sequence comparison and phylogenetic tree, expression in Pichia pastoris and in Escherichia coli
gene CYP76F45, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis and tree, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3). Only simultaneous incubation of the membrane-bound enzymes CsCYP76F45 and CsCPR I with the substrate geraniol and the coenzyme NADPH allows 8-hydroxygeraniol formation
gene G10H, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis. Recombinant overexpression of the ginseng geraniol 10-hydroxylase P450 gene in Arabidopsis thaliana ecotype CS60000, from vector pCAMBIA1390 under control of promoter CaMV 35S, causes terpenoid indole alkaloid dihydrositsirikine production and also confers enhanced resistance to Pseudomonas syringae pv. tomato DC3000
-
gene g10h, quantitative real-time PCR expression analysis
-
overexpression in Catharanthus roseus hairy roots via Agrobacterium rhizogenes 15834 transfection system, co-expression with the 1-deoxy-D-xylulose synthase, DXS, from Arabidopsis thaliana
-
SmG10H, DNA and amno acid sequence determination and analysis, sequence comparisons and phylogenetic tree, semiquantitative RT-PCR expression analysis, recombinant expression in Escherichia coli strain BL21 and in Pichia pastoris