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1.8.4.12: peptide-methionine (R)-S-oxide reductase

This is an abbreviated version!
For detailed information about peptide-methionine (R)-S-oxide reductase, go to the full flat file.

Word Map on EC 1.8.4.12

Reaction

L-methionine (R)-sulfoxide
+
thioredoxin
=
L-methionine
 +
thioredoxin disulfide
 +
H2O

Synonyms

1-Cys methionine sulfoxide reductase B, 1-Cys MSRB, CBS-1, CBS1, cysteine-containing methionine-R-sulfoxide reductase, LOC100305558, LOC100798757, methionine sulfoxide reductase, methionine sulfoxide reductase B, methionine sulfoxide reductase B1, methionine sulfoxide reductase B2, methionine sulfoxide reductase B3, methionine sulfoxide reductase B8, methionine sulfoxide reductase MsrB3, methionine sulfoxide reductases B, methionine sulfoxide reductases B2, methionine sulphoxide reductase, methionine-R-sulfoxide reductase, methionine-R-sulfoxide reductase B, methionine-R-sulfoxide reductase B2, MetO reductase, More, MSR, MsrA, MsrA/B, MsrA/MsrB, msrAB, MsrABTk, MsrB, MSRB1, MsrB2, MsrB3, MsrB3A, MSRB4, MsrB5, MsrB7, MsrB8, MsrBA, Mxr2, NtMsrB2, OsMSRB5, PaMsrB1, peptide methionine sulfoxide reductase, peptide methionine sulfoxide reductase type B, PilB, PilB protein, PMSR, Sel-X, selenocysteine-containing methionine-R-sulfoxide reductase, selenoprotein R, SelR, sulindac reductase, TbmsrB, TCDM_06423, YeaA

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.4 With a disulfide as acceptor
                1.8.4.12 peptide-methionine (R)-S-oxide reductase

Cloned

Cloned on EC 1.8.4.12 - peptide-methionine (R)-S-oxide reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
3 genes msrA and 1 gene msrB form an operon, one of the 3 msrA genes is fused to the msrB gene, genetic organization and regulation, overview
-
CBS-1, overexpression of the His-tagged enzyme in Escherichia coli
chromosome 1 contains 2 genes msrB, chromosome 2 contains 1 gene msrB
-
co-expressed with transient receptor potential melastatin type 6 in HEK-293 cells
-
DNA and amino acid sequence determination and analysis of plastidic isozymes MsrB1 and MsrB2, functional expression in Escherichia coli
-
DNA and amino acid sequence determination and analysis, expression in Escherichia coli
-
endoplasmic reticulum isozyme MsrB3, DNA and amino acid sequence determination and analysis, expression of different constructs of GFP-tagged MsrB3 in monkey kidney CV-1 cells or in mouse fibroblast NIH 3T3 cells
expresion in Escherichia coli BL-21 Star (DE3)
expressed in Drosophila melanogaster
-
expressed in Drosophila melanogaster, in A-549 cells and human dermal fibroblasts
-
expressed in Escherichia coli BL21 (DE3)cells and in Saccharomyces cerevisiae strain BY4741
-
expressed in Escherichia coli BL21(DE3) and in HEK-293 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells and in yeast cytosol
-
expressed in Escherichia coli ER2566 cells
expressed in Escherichia coli M15rep4 cells
-
expression analysis in subcellular fractions of melanocytes, overview
-
expression His-tagged MsrB in Escherichia coli
-
expression in Escherichia coli
expression in Escherichia coli as a fusion protein with GST which is subsequently cleaved after purification
expression in Escherichia coli BL21
expression in Escherichia coli BL21 (DE3)
-
expression in Escherichia coli BL21 (DE3) fused to an C-terminal 6Histag
expression in in Escherichia coli as a fusion with maltose binding protein
expression inm Escherichia coli, recombinant proteins corresponding to MsrABTk and the individual domains (MsrATk and MsrBTk) are produced
-
expression of MsrB in Escherichia coli strain BL21(DE3)
-
expression of the plastidic isozyme pPMSR in Escherichia coli strain BL21(DE3) without the chloroplast signal sequence
-
expression of the wild-type and mutant forms of MsrB in Escherichia coli
-
expression of wild-type and mutant enzymes in Escherichia coli
-
expression of wild-type and mutant isozyme MsrB3 in Escherichia coli strain BL21(DE3)
-
expression of wild-type and mutant isozymes MsrB1 and MsrB2 in Escherichia coli strain BL21(DE3) and in NIH-3T3 cells
-
gene msr or pilB, DNA sequence determination and analysis
-
gene msrB with the codon for selenomehinonine is exchanged for methionine, overexpression in Escherichia coli
-
gene msrB, located in the chromosome at 40.09 min, respectively, regulation mechanism of gene expression, overview
-
gene msrB, overexpression of wild-type enzyme and mutant enzymes in Escherichia coli strain DH5alpha
-
gene OsMSRB5, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, recombinant ectopic expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), recombinant expression of N- or C-terminally sGFP-tagged enzyme in onion epidermal cells
gene pilB, expression of N-terminally His-tagged full-length wild-type and mutant PilB and MsrB domain variants in Escherichia coli
-
gene pilB, transposon insertion, truncated PilB enzyme forms of the enzyme lacking the MsrA domain from strain MS11, variant VD300, overexpression of the His-tagged PilB forms in Escherichia coli strain Xl-1 blue
-
gene selR, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3)
genes msrA and msrB are translationally fused
-
genes msrA, EC 1.8.4.11, and msrB form an operon
-
genes msrB and msrA, EC 1.8.4.11, are translationally fused
-
human MOLT-4 cell line are stably transfected with the pLXSN retroviral expression vector based on the Moloney murine leukemia virus and Moloney murine sarcoma virus to generate a replication-deficient recombinant retrovirus containing the rat MsrA and the human MsrB2 cDNA. The oxidized protein repair enzymes MsrA and MsrB2, when overexpressed in the cells, are able to counteract the zinc-mediated damaging effects
mitochondrial and cytosolic isozymes are encoded on a single gene with 2 initiations sites, delivering an N-terminal signal peptide to the mitochondrial enzyme form
-
msr gene, DNA sequence determination and analysis, subcloning in Escherichia coli strain DH5-alpha, functional complementation of the enzyme-deficient mutant with the wild-type gene
-
MsrB2DELTAS containing one extra amino acid in N-terminal (Met) and a C-terminal His-tag is expressed in Escherichia coli
overexpression in Arabidopsis thaliana
overexpression of MsrB in a yeast strain, expression of MsrB as N-terminally 6His-tagged protein in Escherichia coli strain BL-21
-
overexpression of the full length tandem enzyme, the MsrA, and the MsrB domains, all His-tagged, in Escherichia coli
overexpression of the MsrB domain in Escherichia coli, strain B834(DE3) produces a selenomethionine-substituted MsrB
-
overexpression of truncated wild-type and mutant enzymes as His-tagged proteins in Escherichia coli, functional coexpression of CBS1 in oocytes with Drosophila melanogaster ShC/B potassium channel
-
overexpression of wild-type and mutant enzymes in Escherichia coli
-
overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
overexpression of wild-type and mutants in Escherichia coli, expression as N- or C-terminally 6His-tagged protein lowers the recombinant expression level to 3% of total enzyme expressed, labeling of expressed wild-type with 75SeMet
-
PaMsrB1 as a fusion protein containing an N-terminal maltose-binding protein (MBP) is expressed in Escherichia coli Rosetta (DE3) cells. Production of soluble fusion protein is greater when the cells are cultured at 16 °C than at 37°C
pilB is transcribed as polycistronic transcript, overexpression of PilB as His-tagged protein in Escherichia coliBL21(DE3)
-
subcloning in Escherichia coli
-
the chromosome contains 2 copies of gene msrB, a plasmid harbors 1 copy of gene msrB
-
transfection of MOLT-4 cell line by human MsrB2
yeaA, overexpression of the His-tagged enzyme in Escherichia coli