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2.7.7.50: mRNA guanylyltransferase

This is an abbreviated version!
For detailed information about mRNA guanylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.50

Reaction

GTP
+
(5')ppPur-mRNA
=
diphosphate
 +
G(5')pppPur-mRNA

Synonyms

A103R, A103R protein, cap guanylyltransferase-methyltransferase, capping enzyme, capping enzyme guanylyltransferase, Ceg1, CET1, CmCeg1, D1 protein, GDP polyribonucleotidyltransferase, GlCeg1, GTase, GTP-RNA guanylyltransferase, GTP:RNA GTase, guanylyltransferase, guanylyltransferase mRNA capping, HCE, L protein, Mce1, messenger RNA guanylyltransferase, More, mRNA capping enzyme, mRNA guanylyl transferase, mRNA-cap, mRNA-capping enzyme, NAD-decapping enzyme, nsP1, NudC, PRNTase, protein lambda2, RNA capping enzyme, RNA guanylyltransferase, RNGTT, TbCgm1, TTM-type RTPase-GTase, VACWR106, VP3

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.50 mRNA guanylyltransferase

Purification

Purification on EC 2.7.7.50 - mRNA guanylyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
1000fold, recombinant from Escherichia coli
-
contains little or no RNA 5'-triphosphatase or methyltransferase activity
-
copurifies with S-adenosylmethionine mRNA (guanine-7)-methyltransferase
-
HisTrap column chromatography and Superdex G200 gel filtration
-
large scale, to homogeneity, enzyme has mRNA guanylyltransferase activity
-
mutant enzyme C-terminally truncated to residues 1-545
-
Ni-NTA agarose column chromatography
Lyssavirus rabies
-
Ni-NTA column chromatography and Mono S column chromatography
-
nickel-agarose chromatography
-
partial
physically associated with mRNA 5'-triphosphatase activity
-
recombinant from Spodoptera frugiperda cells after infection with baculovirus, to homogeneity
bluetongue virus serotype 10
-
recombinant from vaccinia virus grown in HeLa cells
-
recombinant His-tagged full length enzyme as well as the recombinant His-tagged N-terminal and C-terminal domains from Escherichia coli BL21(DE3)
recombinant His-tagged wild-type and mutant K177A protein from Bacillus megaterium
-
recombinant His6-tagged A103R protein from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant LEF-4 subunit from overexpressing Spodoptera frugiperda cells
-
recombinant protein
recombinant truncated mutant, residues 438-597, His-tagged
recombinant wild-type and mutant K226A from Trichoplusia ni or Spodoptera frugiperda SF21 cells via baculovirus infection
-
to homogeneity
-