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0.095
2,5-anhydro-D-mannitol 6-phosphate
-
pH 7.5, 30°C
0.025
3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one
inhibition of recombinant PFKFB3 protein, no inhibition of purified PFK-1 activity
8.5 - 13.4
beta-D-fructose 6-phosphate
0.25 - 1.9
dihydroxyacetone phosphate
0.016 - 0.45
glycerate 2-phosphate
0.084
Glycerate 3-phosphate
pH 7.8, 25°C
0.02 - 0.18
glycolate 2-phosphate
2 - 15
N-bromoacetylethanolamine
0.028
o-phthalaldehyde
-
pH 7.4, room temperature
0.014 - 2.3
phosphoenolpyruvate
0.018 - 8.07
sn-glycerol 3-phosphate
additional information
additional information
-
0.008
ATP
-
pH 8.2, 30°C, mutant strain DF905
0.01
ATP
-
pH 8.2, 30°C, mutant strain DF903
8.5
beta-D-fructose 6-phosphate
pH 7.5, 30°C, recombinant HBP1 mutant S460D/T470D
9.2
beta-D-fructose 6-phosphate
pH 7.5, 30°C, recombinant HBP1 mutant S460D
9.5
beta-D-fructose 6-phosphate
pH 7.5, 30°C, recombinant HBP1 mutant H253A/S460D
9.6
beta-D-fructose 6-phosphate
pH 7.5, 30°C, recombinant His-tagged HBP1 deletion mutant
9.7
beta-D-fructose 6-phosphate
pH 7.5, 30°C, recombinant HBP1 mutant S477D
9.9
beta-D-fructose 6-phosphate
pH 7.5, 30°C, recombinant wild-type HBP1
10.9
beta-D-fructose 6-phosphate
pH 7.5, 30°C, recombinant His-tagged HBP1 mutant S302R
11.1
beta-D-fructose 6-phosphate
pH 7.5, 30°C, recombinant HBP1 mutant H253A
11.8
beta-D-fructose 6-phosphate
pH 7.5, 30°C, recombinant His-tagged wild-type HBP2
12.2
beta-D-fructose 6-phosphate
pH 7.5, 30°C, recombinant His-tagged wild-type HBP1
12.7
beta-D-fructose 6-phosphate
pH 7.5, 30°C, recombinant non-phosphorylated, His-tagged chimeric mutant enzyme
13
beta-D-fructose 6-phosphate
pH 7.5, 30°C, recombinant HBP1 mutant T470
13.4
beta-D-fructose 6-phosphate
pH 7.5, 30°C, recombinant phosphorylated, His-tagged chimeric mutant enzyme
0.025
citrate
-
hepatoma cells
0.083
citrate
-
pH 7.1, 30°C, native enzyme
0.084
citrate
-
pH 7.1, 30°C, phosphorylated enzyme
0.12
citrate
-
pH 7.4, 30°C
0.13
citrate
-
pH 7.1, 30°C, phosphorylated enzyme
0.14
citrate
-
skeletal muscle
0.49
citrate
-
pH 7.1, 30°C, native enzyme
0.55
citrate
-
pH 7.5, 30°C
0.25
dihydroxyacetone phosphate
-
L form, pH 7.8, 30°C
0.737
dihydroxyacetone phosphate
pH 7.8, 25°C
1.35
dihydroxyacetone phosphate
pH 6.0, 25°C
1.9
dihydroxyacetone phosphate
-
H form, pH 7.8, 30°C
0.026
diphosphate
-
L form, pH 7.8, 30°C
0.106
diphosphate
pH 7.8, 25°C
0.16
diphosphate
-
H form, pH 7.8, 30°C
0.016
glycerate 2-phosphate
-
L form, pH 7.8, 30°C
0.029
glycerate 2-phosphate
pH 7.8, 25°C
0.14
glycerate 2-phosphate
-
H form, pH 7.8, 30°C
0.45
glycerate 2-phosphate
pH 6.0, 25°C
0.02
glycolate 2-phosphate
-
L form, pH 7.8, 30°C
0.18
glycolate 2-phosphate
-
H form, pH 7.8, 30°C
2
N-bromoacetylethanolamine
-
pH 7.5, 30°C, recombinant testis enzyme
15
N-bromoacetylethanolamine
-
pH 7.5, 30°C, crude liver extracts
0.014
phosphoenolpyruvate
-
pH 7.5, 30°C, testis
0.018
phosphoenolpyruvate
-
L form, pH 7.8, 30°C
0.045
phosphoenolpyruvate
pH 7.8, 25°C
0.08
phosphoenolpyruvate
-
pH 7.5, 25°C, islet
0.08
phosphoenolpyruvate
-
pH 7.5, 30°C, liver
0.1
phosphoenolpyruvate
-
pH 7.5, 30°C, heart
0.12
phosphoenolpyruvate
-
hepatoma cells
0.13
phosphoenolpyruvate
pH 6.0, 25°C
0.15
phosphoenolpyruvate
-
H form, pH 7.8, 30°C
0.17
phosphoenolpyruvate
-
noncompetitive to ATP, pH 7.5, 30°C
0.2
phosphoenolpyruvate
-
liver
0.23
phosphoenolpyruvate
-
pH 7.4, 30°C
0.53
phosphoenolpyruvate
-
noncompetitive to fructose 6-phosphate, pH 7.5, 30°C
0.7
phosphoenolpyruvate
30°C
2
phosphoenolpyruvate
-
30°C
2.3
phosphoenolpyruvate
-
pH 6.6, 25°C
0.018
sn-glycerol 3-phosphate
-
L form, pH 7.8, 30°C
0.15
sn-glycerol 3-phosphate
-
pH 7.1
0.16
sn-glycerol 3-phosphate
-
H form, pH 7.8, 30°C
0.35
sn-glycerol 3-phosphate
pH 6.0, 25°C
8.07
sn-glycerol 3-phosphate
pH 7.8, 25°C
additional information
additional information
-
-
-
additional information
additional information
-
guanidine-inhibition of wild-type and mutant enzymes
-
additional information
additional information
-
comparison of Ki for MgATP2- inhibition at different free Mg2+ concentrations
-
additional information
additional information
-
comparison of Ki of wild type and mutant enzymes
-
additional information
additional information
-
kinetic study
-
additional information
additional information
modeling of inhibition of HBP1 and HBP2 by phosphoenolpyruvate and beta-D-fructose 6-phosphate, Km for phosphorylated wild-type enzyme and mutants with beta-D-fructose 6-phosphate and beta-D-fructose 2,6-bisphosphate
-