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2.7.7.49: RNA-directed DNA polymerase

This is an abbreviated version!
For detailed information about RNA-directed DNA polymerase, go to the full flat file.

Word Map on EC 2.7.7.49

Reaction

a 2'-deoxyribonucleoside 5'-triphosphate
+
DNAn
=
diphosphate
 +
DNAn+1

Synonyms

Bst DNA polymerase, CS5 pol, DNA nucleotidyltransferase (RNA-directed), DNA polymerase, DNA polymerase I, FeLV RT, FIV RT, FV Pol, FV reverse transcriptase, Gag-Pol, HBV-pol, HIV reverse transcriptase, HIV-1 M RT, HIV-1 O RT, HIV-1 R, HIV-1 reverse transcriptase, HIV-1 RT, HIV-reverse transcriptase, HIV-RT, human hepatitis B virus polymerase, human immunodeficiency virus type 1 reverse transcriptase, iScript enzyme, K4 polymerase, K4PolI, M-MuLV reverse transcriptase, MMLV RT, Moloney Murine leukemia virus reverse transcriptase, Moloney murine leukemia virus RT, MoMLV RT, More, MuLV RT, MX162-RT, MX65-RT, nucleoside reverse transcriptase, nucleotidyltransferase, deoxyribonucleate, RNA-dependent, p66 RT, P72, PFV RT, Pol, polymerase/reverse transcriptase, prototype foamy virus reverse transcriptase, R2-RT, reverse transcriptase, reverse transcriptase/RNA dependent DNA polymerase, reverse-transcriptase, revertase, RNA dependent DNA polymerase, RNA revertase, RNA-dependent DNA polymerase, RNA-instructed DNA polymerase, RT, SFV RT, simian foamy virus reverse transcriptase, SS RT, SuperScript I reverse transcriptase, Superscript II, SUPERSCRIPT II reverse transcriptase, T. Z05 pol, telomerase, telomerase catalytic subunit, telomerase reverse transcriptase, TERT, Tgo-Pol, xenotropic murine leukemia virus-related virus reverse transcriptase, XMRV RT, XNA reverse transcriptase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.49 RNA-directed DNA polymerase

Cloned

Cloned on EC 2.7.7.49 - RNA-directed DNA polymerase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
chimeric DNA polymerase, termed CS5 pol, constructed from T. Z05 pol and Tma pol and containing the 5'-3' nuclease domain from Thermus sp. Z05 DNA polymerase (residues 1-291) and the 3'-5' exonuclease and polymerase domains from Thermotoga maritima DNA polymerase (residues 292-893). This chimera retains thermostable DNA polymerase activity, as well as proofreading activity. Using the CS5 chimera, a series of mutant proteins is constructed in which the amino acid side chains are mutated to modulate the 3'-5' exonuclease activity. The chimeric DNA polymerases are overexpressed under the control of the lambda PL promoter are expressed in Escherichia coli
cloned from a new HIV-1 group O isolate from Spain and expressed in Escherichia coli
-
construction of a gene fusion expressing stable fusion protein, expression in Escherichia coli. The resulting gene fusion consists of an open reading frame encoding 698 amino acids. The first 18 amino acids at the N terminus are encoded by the trpE gene, followed by 7 amino acids which are encoded by the pol gene but are not part of the reverse transcriptase. The subsequent 664 amino acids are encoded by the pol gene and the terminal 9 amino acids by pBR322. Construction of deletions at the 3' terminus of the gene results in a 4fold increase in the level of the reverse transcriptase activity in the soluble fraction of crude lysates
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construction of a plasmid that induces in bacteria the synthesis of an enzymatically active reverse transcriptase, expression of a protein with a six-histidine tag in Escherichia coli
-
diverse parts of the sequence coding for reverse-transcriptase are subcloned and expressed in Escherichia coli
-
ectopic expression in the heterohybridoma cell line K6H6/B5
-
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli strain Bli5
-
expression in Escherichia coli
expression in Escherichia coli. The recombinant protein proves to be insoluble and is unable to be recovered from the insoluble fraction of lysates of Escherichia coli. The reverse transcriptase is successfully expressed in a baculovirus vector although yields remained low
expression of A608T/E520G/W827R, M747K/E742K, and M761T/D547G/I584V in Escherichia coli
-
expression of active polyhistidine-tagged reverse transcriptase domain, comprising residues 304-693, in an Escherichia coli/rabbit reticulocyte lysate coupled transcriptase-translation system
-
expression of His-tagged MMLV RT in Escherichia coli strain BL21(DE3)
-
expression of His-tagged protease domain in Escherichia coli strain BL21 (DE3) pREP4:GroESL
-
expression of mutant DNA polymerase I in Escherichia coli strain BL21 AI cytoplasm
-
expression of N-terminal His6-tagged p66/p66 homodimer HIV-1 RT in Escherichia coli strain BL21 (DE3)
-
expression of N-terminally His6-tagged HIV-1 group M and O RT subunits p51 and p66 in Escherichia coli strain BL21(DE3)
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expression of the C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
expression of untagged or C-terminally intein- and biotin-tagged enzyme in Escherichia coli strain BL21 (DE3)
-
expression of wild-type enzyme and mutant enzymes I132A, I132M, I135A, I135M, N136A, N137A, E138A, E138K, T139A, T139V or P140A in Escherichia coli
His-tagged enzyme expression in Escherichia coli strain BL21-pLysS
human LINE-1 ORF2, which encodes reverse transcriptase, is inserted into a baculovirus shuttle vector and expressed in SF21 cells
-
phylogenetic tree, expression of K4PolI in Escherichia coli
-
recombinant enzyme containing the mutations C280S and Q258C is expressed in Escherichia coli
recombinant expression of His-tagged subtype B HIV-1 reverse transcriptase in Escherichia coli strain M15
AF324493, Q8Q2U5, Q8Q2V9
recombinant expression of His-tagged subtype C HIV-1 reverse transcriptase in Escherichia coli strain M15
AF324493, Q8Q2U5, Q8Q2V9
recombinant expression of HIV-1 RTp66/p51 heterodimers, expression of RTp66 subunit in Escherichia coli strain M15 in the absence of HIV-1 protease. The lack of protease allows for the expression of RTp66 without a subsequent cleavage to generate RTp51
-
recombinantly expressed in Escherichia coli
reverse transcriptase variants are cloned from animals infected with SIVmac239 lacking viral protein X
-
the two subunits are cloned and functionally expressed in Escherichia coli. The recombinant proteins are enzymatically active as homodimers, p66 and p51, as well as a heterodimer p66/p51
-
the two subunits of reverse transcriptase are individually expressed in T7 Express Competent Escherichia coli
-
variants of p66 subunit of reverse transcriptase containing meta-Tyr, nor-Tyr, aminomethyl-Phe, and 1-naphthyl-Tyr and 2-naphthyl-Tyr are produced in an Escherichia coli coupled transcription/translation system. Mutant p66 subunits are reconstituted with wild-type p51 subunit of reverse transcriptase
-