1.1.99.18: cellobiose dehydrogenase (acceptor)

This is an abbreviated version!
For detailed information about cellobiose dehydrogenase (acceptor), go to the full flat file.

Word Map on EC 1.1.99.18

Reaction

cellobiose
+
acceptor
=
cellobiono-1,5-lactone
+
reduced acceptor

Synonyms

cbdA, CBO, CBOR, Cdh, CDH IIA, CDH IIB, cdh-1, CDH1, Cdh2, cellobiose (acceptor) 1-oxidoreductase, cellobiose dehydrogenase, cellobiose oxidase, cellobiose oxidoreductase, cellobiose [acceptor] 1-oxidoreductase, Cellobiose-quinone oxidoreductase, cellobiose:(acceptor) 1-oxidoreductase, cellobiose:quinone oxidoreductase, cellobiose:[acceptor] 1-oxidoreductase, DCHsr, dehydrogenase, cellobiose, MtCDH, oxidase, cellobiose, Thite_59724, TpCDH, TvCDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.99 With unknown physiological acceptors
                1.1.99.18 cellobiose dehydrogenase (acceptor)

pH Range

pH Range on EC 1.1.99.18 - cellobiose dehydrogenase (acceptor)

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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.5 - 4.5
lactose as substrate, cytochrome c as electron acceptor
2.5 - 6.5
3 - 5.5
-
highest activity at pH 3, decreases to 10-20% of the activity when the pH increases to pH 5.5, cellobiose + cytochrome c recombinant wild-type enzyme, mutant enzymes N732H, N732Q and N732A
3 - 7
-
highest activity at pH 3, decreases when the pH increases to pH 7.0, cellobiose + 2,6-dichlorophenol-indophenol, to about 10% with recombinant wild-type enzyme, to about 25% with mutant enzymes N732Q, to about 40% with mutant enzyme N732A and to about 50% with mutant enzyme N732H
3 - 5
-
pH 3.0: about 70% of maximal activity, pH 5.0: about 40% of maximal activity, lactose + cytochrome c, in acetate buffer
3 - 6
more than 50% activity between pH 3.0 and 6.0
3 - 5
-
pH 3.0: about 60% of maximal activity, pH 5.0: about 70% of maximal activity, lactose + cytochrome c, in acetate buffer
3.2 - 5.3
pH 3.2: about 50% of maximal activity, pH 5.3: about 45% of maximal activity, reaction with cellobiose and cytochrome c
3.5 - 6
3.5 - 5.5
3.7 - 8
-
more than 50% relative activity from pH 3.8 to 6.5 using 1,4-benzoquinone as electron acceptor
3.8 - 6.5
-
more than 50% relative activity from pH 3.8 to 6.5 using 2,6-dichlorophenolindol as electron acceptor
4 - 7
-
lactose as electron donor, 2,6-diclorindophenol as electron acceptor
4 - 6
-
pH 5.0: about 35% of maximal activity, pH 7.0: about 20% of maximal activity, cellobiose + cytochrome c, mutant enzyme N732E
4 - 6.5
-
the relative activity of purified enzyme is decreased when pH is over 4.5 and it is below 80% at 6.5
4 - 6
-
below pH 4.0, and particularly above pH 6.0, the activity is observed to drop (65% activity at pH 3.0, 45% activity at pH 7.0 and no activity at pH 9.0)
4 - 7
-
at 40°C
4 - 6
4.1 - 5.7
-
pH 4.1: less than 50% of maximum activity, pH 5.7: less than 50% of maximum activity
4.5 - 8
-
pH 4.5: about 60% of maximal activity, pH 8.0: about 40% of maximal activity, reaction with 2,6-dichlorophenol indophenol
5 - 7.5
-
about 50% activity between pH 5.0 and 7.5
5 - 7
-
pH 5.0: about 40% of maximal activity, pH 7.0: about 20% of maximal activity, cellobiose + cytochrome c, mutant enzyme N732D
5.5 - 6
-
the mediated electron transfer currents increase with pH and are 2.3 times lower at pH 5.5 and 6.0 for CDH adsorbed on electrosynthesised polyaniline modified electrode compared to CDH on plain screen printed electrodes
5.5 - 7.5
-
pH 5.0: about 45% of maximal activity, pH 7.0: about 65% of maximal activity, cellobiose + 2,6-dichlorophenol-indophenol, mutant enzyme N732E
5.5 - 8
-
pH 5.5: about 50% of maximal activity, pH 8.0: about 60% of maximal activity, cellobiose + 2,6-dichlorophenol-indophenol, mutant enzyme N732D