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2.4.2.1: purine-nucleoside phosphorylase

This is an abbreviated version!
For detailed information about purine-nucleoside phosphorylase, go to the full flat file.

Word Map on EC 2.4.2.1

Reaction

purine 2'-deoxyribonucleoside
+
phosphate
=
purine
 +
2-deoxy-alpha-D-ribose 1-phosphate

Synonyms

5'-deoxy-5'-methylthioadenosine phosphorylase II, 5'-methythioadenosine phosphorylase, adenine nucleoside phosphorylase, adenosine phosphorylase, AgPNP, AmPNP, ANP, ApMTAP, apPNP, bovPNP, bPNO, CKP1, cytokinin riboside phosphorylase, DeoD, deoD_1, DgPNP, EC592_06680, EcPNP, flB, GsPNPI, GsPNPII, GtPNP, hPNP, HpPNP, HsPNO, HsPNP, Ino-Guo phosphorylase, inosine phosphorylase, inosine-guanosine phosphorylase, KlacPNP, KLLA0_C16621g, MTAPII, MtbPNP, nucleotide phosphatase (2.4.2.1), PfPNP, phosphorylase, purine nucleoside, PiPNP, PNP, PNP 1, PNP 2, PNP I, PNP II, PNP-II, PNP-Zg, PNPase, pNPI, PNPII, Pu-NPase, punA, PUNP, PUNPI, PUNPII, purine deoxynucleoside phosphorylase, purine deoxyribonucleoside phosphorylase, purine nucleoside orthophosphate ribosyl transferase, purine nucleoside phosphorylase, purine nucleoside phosphorylase 1, purine nucleoside phosphorylase 2, purine nucleoside phosphorylase DeoD, purine nucleoside phosphorylase DeoD-type, purine nucleoside phosphorylase II, purine ribonucleoside phosphorylase, purine-nucleoside phosphorylases II, SalT, SSO2343, TgPNP, TTC0194, TTC1070, TtPNPI, TtPNPII, TvPNP, xanthosine phosphorylase, XAP, xapA, ylmD

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.1 purine-nucleoside phosphorylase

KI Value

KI Value on EC 2.4.2.1 - purine-nucleoside phosphorylase

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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000001
(+/-)-cis-1,1-difluoro-2-(tetrahydro-3-piranozyl)ethylphosphonic acid
-
approximate value, with (hypoxanthine-9-yl)methyl aglycone, i.e. Yokomatsu compound
0.0000072 - 0.0000112
(+/-)-cis-1,1-difluoro-2-(tetrahydro-3-piranyl)ethylphosphonic acid with (hypoxanthine-9-yl)methyl aglycone
0.00000182
(3R,4R)-1-((9-deazahypoxanthin-9-yl)methyl)-4-fluoro-4-hydroxymethyl pyrrolidin-3-ol
-
overall dissociation constant
0.01
(3S,4R)-4-(guanin-9-yl)-3-hydroxypyrrolidin-1-N-ylcarbonylphosphonic acid
-
recombinant enzyme, pH 7.5, temperature not specified in the publication
0.000000032
(3S,4S)-1-((9-deazahypoxanthin-9-yl)methyl)-4-fluoro-4-hydroxymethyl pyrrolidin-3-ol
-
overall dissociation constant
0.02
(S)-3-(guanin-9-yl)pyrrolidin-N-ylcarbonylphosphonic acid
-
recombinant enzyme, pH 7.5, temperature not specified in the publication
0.05 - 0.6
1',9-methyl-immucillin-H
0.025
1,6-Dihydropurine riboside
-
-
0.027
1-((2-pyrrolidine-1-yl)ethyl)uracil
pH 7.5, 25°C
0.0021 - 0.0062
1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamidine
0.02
1-methylguanine
-
-
0.0000019 - 0.000019
2'-deoxy-immucillin-H
0.000306
2-amino-1,5-dihydro-7-[[(2S)-2-(aminomethyl)-1-pyrrolidinyl]-methyl]-4H-pyrrolo[3,2-d]pyrimidin-4-one
37°C, pH 7.0
0.000002
2-amino-1,5-dihydro-7-[[(2S)-2-(hydroxymethyl)-1-pyrrolidinyl]methyl]-4H-pyrrolo[3,2-d]pyrimidin-4-one
37°C, pH 7.0, acetic acid salt
0.0000102
2-amino-1,5-dihydro-7-[[[2-(hydroxy)ethyl]amino]methyl]-4H-pyrrolo[3,2-d]pyrimidin-4-one
37°C, pH 7.0
0.0024
2-amino-6-chloro-7-deazapurine 2'-deoxyriboside
-
0.038
2-amino-6-methyl-5,6,7,8-tetrahydropteridin-4(1H)-one
-
-
0.3
2-Amino-6-methylthiopurine
-
-
0.0000000034
2-amino-7-([[(2R,3S)-1,3,4-trihydroxybutan-2-yl]amino]methyl)-3,5-dihydro-4Hpyrrolo[3,2-d]pyrimidin-4-one
-
-
0.0000000021
2-amino-7-[[(1,3-dihydroxypropan-2-yl)amino]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
-
0.0000306
2-amino-7-[[(2-hydroxyethyl)(methyl)amino]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
0.0003163
2-amino-7-[[(2R)-2-(hydroxymethyl)pyrrolidin-1-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
0.0003061
2-amino-7-[[(2S)-2-(methoxymethyl)pyrrolidin-1-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
0.000001
2-amino-7-[[(2S,4R)-4-hydroxy-2-(hydroxymethyl)pyrrolidin-1-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
0.0000018
2-amino-7-[[3-(hydroxymethyl)piperidin-1-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
0.0000025
2-amino-7-[[bis(2-hydroxyethyl)amino]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
0.0001122
2-amino-7-[[ethyl(2-hydroxyethyl)amino]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
0.0014
2-chloro-6-(3-phenyl-1-propoxy)purine
-
0.006
3-((2-pyrrolidine-1-yl)ethyl)uracil
pH 7.5, 25°C
0.0000009 - 0.0015
4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-G
0.0000005 - 0.0036
4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-H
0.0000015
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A
0.000018
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-G
-
-
0.0000000107 - 0.000018
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H
0.00022 - 0.0089
5'-amido-immucillin-H
0.000012 - 0.000017
5'-carboxy-immucillin-H
0.0004 - 0.012
5'-chloro-5'-deoxy-8-aminoguanosine
0.00018
5'-deoxy-5'-iodo-9-deazainosine
-
0.018
5'-deoxy-5'-iodoinosine
-
0.0000000077 - 0.0000016
5'-deoxy-immucillin-H
0.000002 - 0.0000022
5'-fluoro-immucillin-H
0.0000018
5'-methylthio-immucillin H
pH 7.4, temperature not specified in the publication
0.00000027 - 0.0056
5'-methylthio-immucillin-H
0.000000093 - 0.05
5'-thio-immucillin-H
0.008
6-amino-2-chloro-7-deazapurine 2'-deoxyriboside
-
0.005
6-amino-7-phenylethinyl-7-deazapurine 2'-deoxyriboside
-
0.0006
6-benzyloxy-2-chloropurine
-
0.0023
6-chloro-7-deaza-purine 2'-deoxyriboside
-
0.203
6-hydroxy-9-p-aminobenzylpurine
-
-
0.073
6-Mercaptopurine
-
-
0.0003 - 0.0252
6-methylformycin A
0.13
6-methylthiopurine
-
-
1.1
6-Thioxanthine
-
-
0.2
7-Deazaguanine
pH 7.6, 25°C
0.06 - 0.33
7-Deazainosine
0.002 - 0.093
7-ketopyrazolo[4,3-d]pyrimidine
0.082
7-methylguanine
25°C, pH 7.0, with 7-methylguanosine as varied substrate
0.000000016
7-[[(3R,4R)-4-hydroxy-3-(hydroxymethyl)pyrrolidin-2-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
-
0.0033
8-amino-5'-deoxy-5'-iodoguanosine
-
0.0002 - 0.008
8-Amino-9-benzylguanine
0.0002
8-aminoguanine
-
-
0.007 - 0.017
8-aminoguanosine
0.195
8-aza-2,6-diaminopurine
-
-
0.0041
8-bromo-N(9)-acycloguanosine
-
-
0.024 - 0.027
9-(1,3-dihydroxy-2-propoxymethyl)guanine
0.0000075 - 0.0000076
9-(2'-benzyl-5',5'-difluoro-5'-phosphonopentyl)guanine
0.058 - 0.068
9-(2-hydroxyethoxymethyl)guanine
0.0000135
9-(3-pyridylmethyl)-9-deaza-guanosine
pH and temperature not specified in the publication
0.0000044 - 0.0000081
9-(5',5'-difluoro-5'-phosphonobutyl)-9-deazaguanine
0.0000053 - 0.0000057
9-(5',5'-difluoro-5'-phosphonoheptyl)-9-deazaguanine
0.0000033 - 0.0069
9-(5',5'-difluoro-5'-phosphonopentyl)-9-deazaguanine
0.000002
9-(5,5-difluoro-5-phosphonopentyl)guanine
-
approximate value
0.0000056 - 0.0000061
9-deazaguanine
0.028 - 0.11
acyclovir
6.6
adenine
-
-
0.049 - 0.16
adenosine
0.97
allopurinol
-
-
0.0085 - 0.361
alpha-D-ribose 1-phosphate
0.15
aminopterin
-
-
0.063
cis-1,1-difluoro-2-(tetrahydro-3-piranozyl)ethylphosphonic acid
-
wild-type, pH not specified in the publication, temperature not specified in the publication
0.000000066
cis-1-((9-deazahypoxanthin-9-yl)methyl)-4-fluoro-4-hydroxymethylpyrrolidin-3-ol
-
overall dissociation constant
0.000000034 - 0.000000121
DADMe-immucillin-G
0.000000009 - 0.00000035
DADMe-immucillin-H
0.000000009
DATMe-immucillin-H
pH and temperature not specified in the publication
0.0000074 - 0.000682
deoxyimmucillin-H
1.5
deoxyinosine
-
-
0.0000044
DFPP-DG
pH and temperature not specified in the publication
0.33
diphosphate
-
-
0.19
erythro-9-(2-hydroxy-3-nonyl)adenine
-
-
0.0337
folic acid
-
-
0.005
formycin
-
0.0053 - 0.667
Formycin A
0.00039 - 0.705
formycin B
0.0025 - 0.065
guanine
0.01 - 0.8
guanosine
0.0016 - 0.05
hypoxanthine
0.000015
immucillin H
pH 7.4, temperature not specified in the publication
0.000001
immucillin-A
0.000000133 - 0.000346
immucillin-G
0.000000011 - 0.00045
immucillin-H
0.26
Inosine
-
-
0.00000038
L-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H
-
slow-onset binding constant
0.027
N(1)-Methylformycin A
-
pH 7
0.00027 - 0.0003
N(6)-methyl-formycin A
0.018
N(6)-methylformycin B
-
pH 7
0.005 - 0.097
N(7)-acycloguanosine
0.0022
N(7)-methylformycin A
pH 5.5
0.0034 - 0.014
N(9)-acycloguanosine
0.0075
N2,3-etheno-O6-methylguanine
pH 7.3, 25°C
0.038
N2,3-ethenoguanine
pH 7.3, 25°C
0.81
Oxoallopurinol
-
-
0.2
oxoformycin A
-
pH 7
0.18
oxoformycin B
-
pH 7
0.00568
PCMB
-
-
2.5 - 26
phosphate
0.49
purine riboside
-
-
0.323
quinazolinone
-
0.000000005
SerMe-immucillin-H
pH and temperature not specified in the publication
additional information
additional information
-